Identification of a Key Residue for Oligomerisation and Pore-Formation of Clostridium perfringens NetB
Necrotic enteritis toxin B (NetB) is a β-pore-forming toxin produced by Clostridium perfringens and has been identified as a key virulence factor in the pathogenesis of avian necrotic enteritis, a disease causing significant economic damage to the poultry industry worldwide. In this study, site-dire...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2014-03-01
|
| Series: | Toxins |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-6651/6/3/1049 |
| id |
doaj-f70777b14f5141aa8f86f4369883e9a8 |
|---|---|
| record_format |
Article |
| spelling |
doaj-f70777b14f5141aa8f86f4369883e9a82020-11-24T22:38:53ZengMDPI AGToxins2072-66512014-03-01631049106110.3390/toxins6031049toxins6031049Identification of a Key Residue for Oligomerisation and Pore-Formation of Clostridium perfringens NetBSérgio P. Fernandes da Costa0Christos G. Savva1Monika Bokori-Brown2Claire E. Naylor3David S. Moss4Ajit K. Basak5Richard W. Titball6College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter EX4 4QD, UKDepartment of Biological Sciences, School of Crystallography, Institute of Structural and Molecular Biology, Birkbeck College, Malet Street, London WC1E 7HX, UKCollege of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter EX4 4QD, UKDepartment of Biological Sciences, School of Crystallography, Institute of Structural and Molecular Biology, Birkbeck College, Malet Street, London WC1E 7HX, UKDepartment of Biological Sciences, School of Crystallography, Institute of Structural and Molecular Biology, Birkbeck College, Malet Street, London WC1E 7HX, UKDepartment of Biological Sciences, School of Crystallography, Institute of Structural and Molecular Biology, Birkbeck College, Malet Street, London WC1E 7HX, UKCollege of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter EX4 4QD, UKNecrotic enteritis toxin B (NetB) is a β-pore-forming toxin produced by Clostridium perfringens and has been identified as a key virulence factor in the pathogenesis of avian necrotic enteritis, a disease causing significant economic damage to the poultry industry worldwide. In this study, site-directed mutagenesis was used to identify amino acids that play a role in NetB oligomerisation and pore-formation. NetB K41H showed significantly reduced toxicity towards LMH cells and human red blood cells relative to wild type toxin. NetB K41H was unable to oligomerise and form pores in liposomes. These findings suggest that NetB K41H could be developed as a genetic toxoid vaccine to protect against necrotic enteritis.http://www.mdpi.com/2072-6651/6/3/1049NetBpore-forming toxinClostridium perfringensnecrotic enteritis |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Sérgio P. Fernandes da Costa Christos G. Savva Monika Bokori-Brown Claire E. Naylor David S. Moss Ajit K. Basak Richard W. Titball |
| spellingShingle |
Sérgio P. Fernandes da Costa Christos G. Savva Monika Bokori-Brown Claire E. Naylor David S. Moss Ajit K. Basak Richard W. Titball Identification of a Key Residue for Oligomerisation and Pore-Formation of Clostridium perfringens NetB Toxins NetB pore-forming toxin Clostridium perfringens necrotic enteritis |
| author_facet |
Sérgio P. Fernandes da Costa Christos G. Savva Monika Bokori-Brown Claire E. Naylor David S. Moss Ajit K. Basak Richard W. Titball |
| author_sort |
Sérgio P. Fernandes da Costa |
| title |
Identification of a Key Residue for Oligomerisation and Pore-Formation of Clostridium perfringens NetB |
| title_short |
Identification of a Key Residue for Oligomerisation and Pore-Formation of Clostridium perfringens NetB |
| title_full |
Identification of a Key Residue for Oligomerisation and Pore-Formation of Clostridium perfringens NetB |
| title_fullStr |
Identification of a Key Residue for Oligomerisation and Pore-Formation of Clostridium perfringens NetB |
| title_full_unstemmed |
Identification of a Key Residue for Oligomerisation and Pore-Formation of Clostridium perfringens NetB |
| title_sort |
identification of a key residue for oligomerisation and pore-formation of clostridium perfringens netb |
| publisher |
MDPI AG |
| series |
Toxins |
| issn |
2072-6651 |
| publishDate |
2014-03-01 |
| description |
Necrotic enteritis toxin B (NetB) is a β-pore-forming toxin produced by Clostridium perfringens and has been identified as a key virulence factor in the pathogenesis of avian necrotic enteritis, a disease causing significant economic damage to the poultry industry worldwide. In this study, site-directed mutagenesis was used to identify amino acids that play a role in NetB oligomerisation and pore-formation. NetB K41H showed significantly reduced toxicity towards LMH cells and human red blood cells relative to wild type toxin. NetB K41H was unable to oligomerise and form pores in liposomes. These findings suggest that NetB K41H could be developed as a genetic toxoid vaccine to protect against necrotic enteritis. |
| topic |
NetB pore-forming toxin Clostridium perfringens necrotic enteritis |
| url |
http://www.mdpi.com/2072-6651/6/3/1049 |
| work_keys_str_mv |
AT sergiopfernandesdacosta identificationofakeyresidueforoligomerisationandporeformationofclostridiumperfringensnetb AT christosgsavva identificationofakeyresidueforoligomerisationandporeformationofclostridiumperfringensnetb AT monikabokoribrown identificationofakeyresidueforoligomerisationandporeformationofclostridiumperfringensnetb AT claireenaylor identificationofakeyresidueforoligomerisationandporeformationofclostridiumperfringensnetb AT davidsmoss identificationofakeyresidueforoligomerisationandporeformationofclostridiumperfringensnetb AT ajitkbasak identificationofakeyresidueforoligomerisationandporeformationofclostridiumperfringensnetb AT richardwtitball identificationofakeyresidueforoligomerisationandporeformationofclostridiumperfringensnetb |
| _version_ |
1725711301959745536 |