DETERMINATION OF VANILLIN IN VANILLA (Vanilla planifolia Andrews) FROM LAMPUNG INDONESIA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

DETERMINATION OF VANILLIN IN VANILLA (Vanilla planifolia Andrews) FROM LAMPUNG INDONESIA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

This paper describes a reverse-phase high performance liquid chromatographic (HPLC) method for the separation, identification and quantification of vanillin in ethanolic extracts of cured vanilla. The fresh green beans were cured by three methods: scalding in hot water, drying in the oven, and dryin...

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Main Authors: Siti Sujalmi, Suharso Suharso, R Supriyanto, Buchari Buchari
Format: Article
Language:English
Published: Universitas Gadjah Mada 2010-06-01
Series:Indonesian Journal of Chemistry
Online Access:https://jurnal.ugm.ac.id/ijc/article/view/21831
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spelling doaj-f7233d7a909a4293b4efe836348fa6a22020-11-25T00:26:37ZengUniversitas Gadjah MadaIndonesian Journal of Chemistry1411-94202460-15782010-06-015171010.22146/ijc.2183114929DETERMINATION OF VANILLIN IN VANILLA (Vanilla planifolia Andrews) FROM LAMPUNG INDONESIA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHYSiti Sujalmi0Suharso Suharso1R Supriyanto2Buchari Buchari3Department of Chemistry, University of Lampung, Bandar Lampung, Indonesia b Department of Chemistry, Bandung Institute of TechnologyDepartment of Chemistry, University of Lampung, Bandar Lampung, Indonesia b Department of Chemistry, Bandung Institute of TechnologyDepartment of Chemistry, University of Lampung, Bandar Lampung, Indonesia b Department of Chemistry, Bandung Institute of TechnologyDepartment of Chemistry, Bandung Institute of TechnologyThis paper describes a reverse-phase high performance liquid chromatographic (HPLC) method for the separation, identification and quantification of vanillin in ethanolic extracts of cured vanilla. The fresh green beans were cured by three methods: scalding in hot water, drying in the oven, and drying in the sun. Two treatments for the cured beans before extraction, there were cutting cured vanilla about 2.5 cm and not cutting. The extraction was with Soxhletation and percolation method in 99.9 % ethanol. The vanillin was separated on C18 column using a mobile phase gradient of methanol  - acidified water (10-90), detection at 280 nm. The HPLC technique allows a more accurate means of determining the vanillin  content of vanilla than  the spectrophotometric method .   Keywords: Vanilla planifolia Andrews, Vanillin, HPLChttps://jurnal.ugm.ac.id/ijc/article/view/21831
collection DOAJ
language English
format Article
sources DOAJ
author Siti Sujalmi
Suharso Suharso
R Supriyanto
Buchari Buchari
spellingShingle Siti Sujalmi
Suharso Suharso
R Supriyanto
Buchari Buchari
DETERMINATION OF VANILLIN IN VANILLA (Vanilla planifolia Andrews) FROM LAMPUNG INDONESIA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Indonesian Journal of Chemistry
author_facet Siti Sujalmi
Suharso Suharso
R Supriyanto
Buchari Buchari
author_sort Siti Sujalmi
title DETERMINATION OF VANILLIN IN VANILLA (Vanilla planifolia Andrews) FROM LAMPUNG INDONESIA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
title_short DETERMINATION OF VANILLIN IN VANILLA (Vanilla planifolia Andrews) FROM LAMPUNG INDONESIA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
title_full DETERMINATION OF VANILLIN IN VANILLA (Vanilla planifolia Andrews) FROM LAMPUNG INDONESIA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
title_fullStr DETERMINATION OF VANILLIN IN VANILLA (Vanilla planifolia Andrews) FROM LAMPUNG INDONESIA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
title_full_unstemmed DETERMINATION OF VANILLIN IN VANILLA (Vanilla planifolia Andrews) FROM LAMPUNG INDONESIA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
title_sort determination of vanillin in vanilla (vanilla planifolia andrews) from lampung indonesia by high performance liquid chromatography
publisher Universitas Gadjah Mada
series Indonesian Journal of Chemistry
issn 1411-9420
2460-1578
publishDate 2010-06-01
description This paper describes a reverse-phase high performance liquid chromatographic (HPLC) method for the separation, identification and quantification of vanillin in ethanolic extracts of cured vanilla. The fresh green beans were cured by three methods: scalding in hot water, drying in the oven, and drying in the sun. Two treatments for the cured beans before extraction, there were cutting cured vanilla about 2.5 cm and not cutting. The extraction was with Soxhletation and percolation method in 99.9 % ethanol. The vanillin was separated on C18 column using a mobile phase gradient of methanol  - acidified water (10-90), detection at 280 nm. The HPLC technique allows a more accurate means of determining the vanillin  content of vanilla than  the spectrophotometric method .   Keywords: Vanilla planifolia Andrews, Vanillin, HPLC
url https://jurnal.ugm.ac.id/ijc/article/view/21831
work_keys_str_mv AT sitisujalmi determinationofvanillininvanillavanillaplanifoliaandrewsfromlampungindonesiabyhighperformanceliquidchromatography
AT suharsosuharso determinationofvanillininvanillavanillaplanifoliaandrewsfromlampungindonesiabyhighperformanceliquidchromatography
AT rsupriyanto determinationofvanillininvanillavanillaplanifoliaandrewsfromlampungindonesiabyhighperformanceliquidchromatography
AT bucharibuchari determinationofvanillininvanillavanillaplanifoliaandrewsfromlampungindonesiabyhighperformanceliquidchromatography
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