Controlled release of TGF-beta 1 from RADA self-assembling peptide hydrogel scaffolds

• Main Authors: Zhou A, Chen S, He B, Zhao W, Chen X, Jiang D
• Format: Article
• Language: English
• Published: Dove Medical Press 2016-09-01
• Series: Drug Design, Development and Therapy
• Subjects: Molecular self-assembly; self-assembling peptide; hydrogel scaffolds; controlled release; TGF-beta 1; bone mesenchymal stem cells.
• Online Access: https://www.dovepress.com/controlled-release-of-transforming-growth-factor-beta-1-from-rada-self-peer-reviewed-article-DDDT

Ao Zhou,1 Shuo Chen,1,2 Bin He,1 Weikang Zhao,1 Xiaojun Chen,1 Dianming Jiang1 1Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 2Department of Orthopedics, Dujiangyan Medical Center, Dujiangyan, People’s Republic of China Abstract: Bioactive mediators, cytokines, and chemokines have an important role in regulating and optimizing the synergistic action of materials, cells, and cellular microenvironments for tissue engineering. RADA self-assembling peptide hydrogels have been proved to have an excellent ability to promote cell proliferation, wound healing, tissue repair, and drug delivery. Here, we report that D-RADA16 and L-RADA16-RGD self-assembling peptides can form stable second structure and hydrogel scaffolds, affording the slow release of growth factor (transforming growth factor cytokine-beta 1 [TGF-beta 1]). In vitro tests demonstrated that the plateau release amount can be obtained till 72 hours. Moreover, L-RADA16, D-RADA16, and L-RADA16-RGD self-assembling peptide hydrogels containing TGF-beta 1 were used for 3D cell culture of bone mesenchymal stem cells of rats for 2 weeks. The results revealed that these three RADA16 peptide hydrogels had a significantly favorable influence on proliferation of bone mesenchymal stem cells and hold some promise in slow and sustained release of growth factor. Keywords: molecular self-assembly, self-assembling peptide, hydrogel scaffolds, controlled release, TGF-beta 1, bone mesenchymal stem cells