Monitoring perinatal gut microbiota in mouse models by mass spectrometry approaches: parental genetic background and breastfeeding effects
At birth, contact with external stimuli, such as nutrients derived from food, is necessary to modulate the symbiotic balance between commensal and pathogenic bacteria, protect against bacterial dysbiosis, and initiate the development of the mucosal immune response. Among a variety of different feedi...
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doaj-f72d0ac0cbf5462fabdcda033a6064012020-11-25T00:04:46ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-09-01710.3389/fmicb.2016.01523206163Monitoring perinatal gut microbiota in mouse models by mass spectrometry approaches: parental genetic background and breastfeeding effectsStefano Levi Mortera0Federica Del Chierico1Pamela Vernocchi2Maria Manuela Rosado3Agnese Cavola4Marco Chierici5Luisa Pieroni6Andrea Urbani7Andrea Urbani8Rita Carsetti9Isabella Lante10Bruno Dallapiccola11Lorenza Putignani12Lorenza Putignani13Bambino Gesù Children's HospitalBambino Gesù Children's HospitalBambino Gesù Children's HospitalBambino Gesù Children's HospitalUniversity of Rome Tor Vergata Bruno Kessler FoundationFondazione Santa LuciaUniversity of Rome Tor VergataFondazione Santa LuciaBambino Gesù Children's HospitalSan Camillo HospitalBambino Gesù Children's HospitalBambino Gesù Children's HospitalBambino Gesù Children's HospitalAt birth, contact with external stimuli, such as nutrients derived from food, is necessary to modulate the symbiotic balance between commensal and pathogenic bacteria, protect against bacterial dysbiosis, and initiate the development of the mucosal immune response. Among a variety of different feeding patterns, breastfeeding represents the best modality. In fact, the capacity of breast milk to modulate the composition of infants’ gut microbiota leads to beneficial effects on their health. In this study, we used newborn mice as a model to evaluate the effect of parental genetic background (i.e., IgA-producing mice and IgA-deficient mice) and feeding modulation (i.e., maternal feeding and cross-feeding) on the onset and shaping of gut microbiota after birth. To investigate these topics, we used either a culturomic approach that employed Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MS), or bottom-up Liquid Chromatography, with subsequent MSMS shotgun metaproteomic analysis that compared and assembled results of the two techniques. We found that the microbial community was enriched by lactic acid bacteria when pups were breastfed by wild-type (WT) mothers, while IgA-deficient milk led to an increase in the opportunistic bacterial pathogen (OBP) population. Cross-feeding results suggested that IgA supplementation promoted the exclusion of some OBPs and the temporary appearance of beneficial species in pups fed by WT foster mothers. Our results show that both techniques yield a picture of microbiota from different angles and with varying depths. In particular, our metaproteomic pipeline was found to be a reliable tool in the description of microbiota. Data from these studies are available via ProteomeXchange, with identifier PXD004033.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01523/fullMouse Gut Microbiota (MGM)MGM metaproteomicsMGM programming phylotypesmaternal milk induced metaproteomicsaxenic culture-based MALDI-TOF MS |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Stefano Levi Mortera Federica Del Chierico Pamela Vernocchi Maria Manuela Rosado Agnese Cavola Marco Chierici Luisa Pieroni Andrea Urbani Andrea Urbani Rita Carsetti Isabella Lante Bruno Dallapiccola Lorenza Putignani Lorenza Putignani |
spellingShingle |
Stefano Levi Mortera Federica Del Chierico Pamela Vernocchi Maria Manuela Rosado Agnese Cavola Marco Chierici Luisa Pieroni Andrea Urbani Andrea Urbani Rita Carsetti Isabella Lante Bruno Dallapiccola Lorenza Putignani Lorenza Putignani Monitoring perinatal gut microbiota in mouse models by mass spectrometry approaches: parental genetic background and breastfeeding effects Frontiers in Microbiology Mouse Gut Microbiota (MGM) MGM metaproteomics MGM programming phylotypes maternal milk induced metaproteomics axenic culture-based MALDI-TOF MS |
author_facet |
Stefano Levi Mortera Federica Del Chierico Pamela Vernocchi Maria Manuela Rosado Agnese Cavola Marco Chierici Luisa Pieroni Andrea Urbani Andrea Urbani Rita Carsetti Isabella Lante Bruno Dallapiccola Lorenza Putignani Lorenza Putignani |
author_sort |
Stefano Levi Mortera |
title |
Monitoring perinatal gut microbiota in mouse models by mass spectrometry approaches: parental genetic background and breastfeeding effects |
title_short |
Monitoring perinatal gut microbiota in mouse models by mass spectrometry approaches: parental genetic background and breastfeeding effects |
title_full |
Monitoring perinatal gut microbiota in mouse models by mass spectrometry approaches: parental genetic background and breastfeeding effects |
title_fullStr |
Monitoring perinatal gut microbiota in mouse models by mass spectrometry approaches: parental genetic background and breastfeeding effects |
title_full_unstemmed |
Monitoring perinatal gut microbiota in mouse models by mass spectrometry approaches: parental genetic background and breastfeeding effects |
title_sort |
monitoring perinatal gut microbiota in mouse models by mass spectrometry approaches: parental genetic background and breastfeeding effects |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2016-09-01 |
description |
At birth, contact with external stimuli, such as nutrients derived from food, is necessary to modulate the symbiotic balance between commensal and pathogenic bacteria, protect against bacterial dysbiosis, and initiate the development of the mucosal immune response. Among a variety of different feeding patterns, breastfeeding represents the best modality. In fact, the capacity of breast milk to modulate the composition of infants’ gut microbiota leads to beneficial effects on their health. In this study, we used newborn mice as a model to evaluate the effect of parental genetic background (i.e., IgA-producing mice and IgA-deficient mice) and feeding modulation (i.e., maternal feeding and cross-feeding) on the onset and shaping of gut microbiota after birth. To investigate these topics, we used either a culturomic approach that employed Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MS), or bottom-up Liquid Chromatography, with subsequent MSMS shotgun metaproteomic analysis that compared and assembled results of the two techniques. We found that the microbial community was enriched by lactic acid bacteria when pups were breastfed by wild-type (WT) mothers, while IgA-deficient milk led to an increase in the opportunistic bacterial pathogen (OBP) population. Cross-feeding results suggested that IgA supplementation promoted the exclusion of some OBPs and the temporary appearance of beneficial species in pups fed by WT foster mothers. Our results show that both techniques yield a picture of microbiota from different angles and with varying depths. In particular, our metaproteomic pipeline was found to be a reliable tool in the description of microbiota. Data from these studies are available via ProteomeXchange, with identifier PXD004033. |
topic |
Mouse Gut Microbiota (MGM) MGM metaproteomics MGM programming phylotypes maternal milk induced metaproteomics axenic culture-based MALDI-TOF MS |
url |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01523/full |
work_keys_str_mv |
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