Patient-Derived Tumor Organoid Rings for Histologic Characterization and High-Throughput Screening

Patient-Derived Tumor Organoid Rings for Histologic Characterization and High-Throughput Screening

Summary: Tumor organoids are promising tools for cancer biology investigations and pre-clinical drug screenings because they are often representative of the histology and drug responses of patients. Here, we introduce a facile protocol to overcome technical limitations by generating patient-derived...

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Main Authors: Huyen Thi Lam Nguyen, Alice Soragni
Format: Article
Language:English
Published: Elsevier 2020-09-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720300435
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spelling doaj-f74a4ec4fc3842e4ac4f2f67ba01431c2020-11-25T04:09:05ZengElsevierSTAR Protocols2666-16672020-09-0112100056Patient-Derived Tumor Organoid Rings for Histologic Characterization and High-Throughput ScreeningHuyen Thi Lam Nguyen0Alice Soragni1Department of Orthopaedic Surgery, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USADepartment of Orthopaedic Surgery, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA; Molecular Biology Institute (MBI), University of California, Los Angeles, Los Angeles, CA, USA; Jonsson Comprehensive Cancer Center (JCCC), University of California, Los Angeles, Los Angeles, CA, USA; California NanoSystems Institute (CNSI), University of California, Los Angeles, Los Angeles, CA, USA; Corresponding authorSummary: Tumor organoids are promising tools for cancer biology investigations and pre-clinical drug screenings because they are often representative of the histology and drug responses of patients. Here, we introduce a facile protocol to overcome technical limitations by generating patient-derived tumor organoids using a simplified ring-like geometry. This facilitates media exchange and drug treatment for histopathology characterization and automated high-throughput drug screenings.For complete details on the use and execution of this protocol, please refer to Phan et al. (2019).http://www.sciencedirect.com/science/article/pii/S2666166720300435
collection DOAJ
language English
format Article
sources DOAJ
author Huyen Thi Lam Nguyen
Alice Soragni
spellingShingle Huyen Thi Lam Nguyen
Alice Soragni
Patient-Derived Tumor Organoid Rings for Histologic Characterization and High-Throughput Screening
STAR Protocols
author_facet Huyen Thi Lam Nguyen
Alice Soragni
author_sort Huyen Thi Lam Nguyen
title Patient-Derived Tumor Organoid Rings for Histologic Characterization and High-Throughput Screening
title_short Patient-Derived Tumor Organoid Rings for Histologic Characterization and High-Throughput Screening
title_full Patient-Derived Tumor Organoid Rings for Histologic Characterization and High-Throughput Screening
title_fullStr Patient-Derived Tumor Organoid Rings for Histologic Characterization and High-Throughput Screening
title_full_unstemmed Patient-Derived Tumor Organoid Rings for Histologic Characterization and High-Throughput Screening
title_sort patient-derived tumor organoid rings for histologic characterization and high-throughput screening
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2020-09-01
description Summary: Tumor organoids are promising tools for cancer biology investigations and pre-clinical drug screenings because they are often representative of the histology and drug responses of patients. Here, we introduce a facile protocol to overcome technical limitations by generating patient-derived tumor organoids using a simplified ring-like geometry. This facilitates media exchange and drug treatment for histopathology characterization and automated high-throughput drug screenings.For complete details on the use and execution of this protocol, please refer to Phan et al. (2019).
url http://www.sciencedirect.com/science/article/pii/S2666166720300435
work_keys_str_mv AT huyenthilamnguyen patientderivedtumororganoidringsforhistologiccharacterizationandhighthroughputscreening
AT alicesoragni patientderivedtumororganoidringsforhistologiccharacterizationandhighthroughputscreening
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