Digital PCR: A Reliable Tool for Analyzing and Monitoring Hematologic Malignancies
The digital polymerase chain reaction (dPCR) is considered to be the third-generation polymerase chain reaction (PCR), as it yields direct, absolute and precise measures of target sequences. dPCR has proven particularly useful for the accurate detection and quantification of low-abundance nucleic ac...
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2020-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/9/3141 |
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doaj-f74e2944ebec46dfb54a18d3018934ab2020-11-25T02:53:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-04-01213141314110.3390/ijms21093141Digital PCR: A Reliable Tool for Analyzing and Monitoring Hematologic MalignanciesNicoletta Coccaro0Giuseppina Tota1Luisa Anelli2Antonella Zagaria3Giorgina Specchia4Francesco Albano5Department of Emergency and Organ Transplantation (D.E.T.O.), Hematology Section, University of Bari, 70124 Bari, ItalyDepartment of Emergency and Organ Transplantation (D.E.T.O.), Hematology Section, University of Bari, 70124 Bari, ItalyDepartment of Emergency and Organ Transplantation (D.E.T.O.), Hematology Section, University of Bari, 70124 Bari, ItalyDepartment of Emergency and Organ Transplantation (D.E.T.O.), Hematology Section, University of Bari, 70124 Bari, ItalyDepartment of Emergency and Organ Transplantation (D.E.T.O.), Hematology Section, University of Bari, 70124 Bari, ItalyDepartment of Emergency and Organ Transplantation (D.E.T.O.), Hematology Section, University of Bari, 70124 Bari, ItalyThe digital polymerase chain reaction (dPCR) is considered to be the third-generation polymerase chain reaction (PCR), as it yields direct, absolute and precise measures of target sequences. dPCR has proven particularly useful for the accurate detection and quantification of low-abundance nucleic acids, highlighting its advantages in cancer diagnosis and in predicting recurrence and monitoring minimal residual disease, mostly coupled with next generation sequencing. In the last few years, a series of studies have employed dPCR for the analysis of hematologic malignancies. In this review, we will summarize these findings, attempting to focus on the potential future perspectives of the application of this promising technology.https://www.mdpi.com/1422-0067/21/9/3141digital PCRdPCRnext-generation sequencingNGShematologysomatic mutation |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Nicoletta Coccaro Giuseppina Tota Luisa Anelli Antonella Zagaria Giorgina Specchia Francesco Albano |
| spellingShingle |
Nicoletta Coccaro Giuseppina Tota Luisa Anelli Antonella Zagaria Giorgina Specchia Francesco Albano Digital PCR: A Reliable Tool for Analyzing and Monitoring Hematologic Malignancies International Journal of Molecular Sciences digital PCR dPCR next-generation sequencing NGS hematology somatic mutation |
| author_facet |
Nicoletta Coccaro Giuseppina Tota Luisa Anelli Antonella Zagaria Giorgina Specchia Francesco Albano |
| author_sort |
Nicoletta Coccaro |
| title |
Digital PCR: A Reliable Tool for Analyzing and Monitoring Hematologic Malignancies |
| title_short |
Digital PCR: A Reliable Tool for Analyzing and Monitoring Hematologic Malignancies |
| title_full |
Digital PCR: A Reliable Tool for Analyzing and Monitoring Hematologic Malignancies |
| title_fullStr |
Digital PCR: A Reliable Tool for Analyzing and Monitoring Hematologic Malignancies |
| title_full_unstemmed |
Digital PCR: A Reliable Tool for Analyzing and Monitoring Hematologic Malignancies |
| title_sort |
digital pcr: a reliable tool for analyzing and monitoring hematologic malignancies |
| publisher |
MDPI AG |
| series |
International Journal of Molecular Sciences |
| issn |
1661-6596 1422-0067 |
| publishDate |
2020-04-01 |
| description |
The digital polymerase chain reaction (dPCR) is considered to be the third-generation polymerase chain reaction (PCR), as it yields direct, absolute and precise measures of target sequences. dPCR has proven particularly useful for the accurate detection and quantification of low-abundance nucleic acids, highlighting its advantages in cancer diagnosis and in predicting recurrence and monitoring minimal residual disease, mostly coupled with next generation sequencing. In the last few years, a series of studies have employed dPCR for the analysis of hematologic malignancies. In this review, we will summarize these findings, attempting to focus on the potential future perspectives of the application of this promising technology. |
| topic |
digital PCR dPCR next-generation sequencing NGS hematology somatic mutation |
| url |
https://www.mdpi.com/1422-0067/21/9/3141 |
| work_keys_str_mv |
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