Effect of Amniotic Membrane Homogenate Homogenate on Ischemic Skin Flap Survival in Rat Model

• Main Authors: Gh. Khorasani, D. Nazemi Salman, A. Atri, K. Ghanbarzadeh, N. Rahman Panah
• Format: Article
• Language: fas
• Published: Alborz University of Medical Sciencs 2015-02-01
• Series: Bihdād
• Subjects: Amniotic membrane homogenate; Ischemic skin flaps; Necrosis; Survival
• Online Access: http://aums.abzums.ac.ir/browse.php?a_code=A-10-1-97&slc_lang=en&sid=1

Bachground: Surgical skin flaps are frequently used in plastic and reconstructive surgery to repair acquired or congenital defects. Necrosis is a common complication associated with these flaps postoperatively as a result of inadequate blood supply. This study was designed to evaluate the effectiveness of amniotic membrane homogenate in healing the dorsal skin of the rat as an ischemic flap model. Materials and Methods: In this experimental study, twenty male Wistar rats weighing between 350 and 400 g were used in the study. For all rats 3 * 10 cm, full-thickness, caudally based pattern dorsal skin flaps were elevated. The rats were randomized into 2 groups: control (I) and experimental (II). Then in 10 point,0.1 mL amniotic membrane homogenate was injected with with equal interval in experimental group and normal salin administered in control group. Seven days later all skin flaps were examined mechanically (necrosis) and histologically. Results: Tissue flap necrosis showed significantly reduced in amniotic membrane homogenate group (p=0.032), and tissue flap survival showed significantly increased in amniotic membrane homogenate group (p=0.036). There were no significant differences in the histology analysis heling proccess between two groups (p>0.05). Conclusion: It seems that, the administration of amniotic membrane homogenate is an effective and safe method that improves the survival of ischemic skin flaps in a rat model.