Activity Components from <i>Gynostemma pentaphyllum</i> for Preventing Hepatic Fibrosis and of Its Molecular Targets by Network Pharmacology Approach

Activity Components from <i>Gynostemma pentaphyllum</i> for Preventing Hepatic Fibrosis and of Its Molecular Targets by Network Pharmacology Approach

Hepatic fibrosis would develop into cirrhosis or cancer without treating. Hence, it is necessary to study the mechanism and prevention methods for hepatic fibrosis. <i>Gynostemma pentaphyllum</i> is a traditional medicinal material with a high medicinal and health value. In this study, n...

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Main Authors: Yumeng Zhang, Guohui Shi, Zhonghua Luo, Jiewen Wang, Shao Wu, Xiaoshu Zhang, Yuqing Zhao
Format: Article
Language:English
Published: MDPI AG 2021-05-01
Series:Molecules
Subjects:
<i>G. pentaphyllum</i>
hepatic fibrosis
network pharmacology
PI3K/Akt signaling pathway
Online Access:https://www.mdpi.com/1420-3049/26/10/3006
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spelling doaj-f759b30c76024a209ff08225d06597c52021-06-01T00:24:22ZengMDPI AGMolecules1420-30492021-05-01263006300610.3390/molecules26103006Activity Components from <i>Gynostemma pentaphyllum</i> for Preventing Hepatic Fibrosis and of Its Molecular Targets by Network Pharmacology ApproachYumeng Zhang0Guohui Shi1Zhonghua Luo2Jiewen Wang3Shao Wu4Xiaoshu Zhang5Yuqing Zhao6School of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang 110016, ChinaHepatic fibrosis would develop into cirrhosis or cancer without treating. Hence, it is necessary to study the mechanism and prevention methods for hepatic fibrosis. <i>Gynostemma pentaphyllum</i> is a traditional medicinal material with a high medicinal and health value. In this study, nineteen compounds obtained from <i>G. pentaphyllum</i> were qualitative and quantitative by HPLC-FT-ICR MS and HPLC-UV, respectively. Among them, the total content of 19 gypenosides accurately quantified reaches 72.21 mg/g and their anti-proliferation against t-HSC/Cl-6 cells indicated compound <b>19</b> performed better activity (IC50: 28.1 ± 2.0 μM) than the other compounds. Further network pharmacology study demonstrated that compound <b>19</b> mainly plays an anti-fibrosis role by regulating the EGFR signaling pathway, and the PI3K–Akt signaling pathway. Overall, the verification result indicated that compound <b>19</b> appeared to be nontoxic to LO2, was able to modulate the PI3K/Akt signal, led to subG1 cells cycle arrest and the activation of mitochondrial-mediated apoptosis of t-HSC/Cl-6 cells for anti-hepatic fibrosis.https://www.mdpi.com/1420-3049/26/10/3006<i>G. pentaphyllum</i>hepatic fibrosisnetwork pharmacologyPI3K/Akt signaling pathway
collection DOAJ
language English
format Article
sources DOAJ
author Yumeng Zhang
Guohui Shi
Zhonghua Luo
Jiewen Wang
Shao Wu
Xiaoshu Zhang
Yuqing Zhao
spellingShingle Yumeng Zhang
Guohui Shi
Zhonghua Luo
Jiewen Wang
Shao Wu
Xiaoshu Zhang
Yuqing Zhao
Activity Components from <i>Gynostemma pentaphyllum</i> for Preventing Hepatic Fibrosis and of Its Molecular Targets by Network Pharmacology Approach
Molecules
<i>G. pentaphyllum</i>
hepatic fibrosis
network pharmacology
PI3K/Akt signaling pathway
author_facet Yumeng Zhang
Guohui Shi
Zhonghua Luo
Jiewen Wang
Shao Wu
Xiaoshu Zhang
Yuqing Zhao
author_sort Yumeng Zhang
title Activity Components from <i>Gynostemma pentaphyllum</i> for Preventing Hepatic Fibrosis and of Its Molecular Targets by Network Pharmacology Approach
title_short Activity Components from <i>Gynostemma pentaphyllum</i> for Preventing Hepatic Fibrosis and of Its Molecular Targets by Network Pharmacology Approach
title_full Activity Components from <i>Gynostemma pentaphyllum</i> for Preventing Hepatic Fibrosis and of Its Molecular Targets by Network Pharmacology Approach
title_fullStr Activity Components from <i>Gynostemma pentaphyllum</i> for Preventing Hepatic Fibrosis and of Its Molecular Targets by Network Pharmacology Approach
title_full_unstemmed Activity Components from <i>Gynostemma pentaphyllum</i> for Preventing Hepatic Fibrosis and of Its Molecular Targets by Network Pharmacology Approach
title_sort activity components from <i>gynostemma pentaphyllum</i> for preventing hepatic fibrosis and of its molecular targets by network pharmacology approach
publisher MDPI AG
series Molecules
issn 1420-3049
publishDate 2021-05-01
description Hepatic fibrosis would develop into cirrhosis or cancer without treating. Hence, it is necessary to study the mechanism and prevention methods for hepatic fibrosis. <i>Gynostemma pentaphyllum</i> is a traditional medicinal material with a high medicinal and health value. In this study, nineteen compounds obtained from <i>G. pentaphyllum</i> were qualitative and quantitative by HPLC-FT-ICR MS and HPLC-UV, respectively. Among them, the total content of 19 gypenosides accurately quantified reaches 72.21 mg/g and their anti-proliferation against t-HSC/Cl-6 cells indicated compound <b>19</b> performed better activity (IC50: 28.1 ± 2.0 μM) than the other compounds. Further network pharmacology study demonstrated that compound <b>19</b> mainly plays an anti-fibrosis role by regulating the EGFR signaling pathway, and the PI3K–Akt signaling pathway. Overall, the verification result indicated that compound <b>19</b> appeared to be nontoxic to LO2, was able to modulate the PI3K/Akt signal, led to subG1 cells cycle arrest and the activation of mitochondrial-mediated apoptosis of t-HSC/Cl-6 cells for anti-hepatic fibrosis.
topic <i>G. pentaphyllum</i>
hepatic fibrosis
network pharmacology
PI3K/Akt signaling pathway
url https://www.mdpi.com/1420-3049/26/10/3006
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