Assessment of genetic diversity in sorghum bicolor using RAPD markers
Sorghum is one of the most important cereal crop and ranks fifth among cereals after wheat, rice, maize and barley for economic importance. Because the demand of food is increasing, sorghum will increase in importance as a source of food, feed, fibre, and fuel; specially in the European con...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
Serbian Genetics Society
2019-01-01
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Series: | Genetika |
Subjects: | |
Online Access: | http://www.doiserbia.nb.rs/img/doi/0534-0012/2019/0534-00121903789R.pdf |
Summary: | Sorghum is one of the most important cereal crop and ranks fifth among
cereals after wheat, rice, maize and barley for economic importance. Because
the demand of food is increasing, sorghum will increase in importance as a
source of food, feed, fibre, and fuel; specially in the European continent
where sorghum is little cultivated, mainly due to the lack of sorghum
varieties well adapted to the soil and climate conditions such as
photoperiod, cold and drought; for this reason, the genetic diversity
analysis, through molecular characterization, is an important requirement to
begin a plant breeding program. The analysis was performed in 46 sorghum
genotypes obtained from the Czech Plant Gene Bank, Crop Research Institute,
Prague. Genetic variability values were estimated, through the genetic
distance using Dice’s coefficient, and dendrogram constructed using DARwing
software. Four out of fifteen of the primers evaluated were completely
polymorphic (100%), A hundred and twenty-six scorable bands were identified
and 89% of them were polymorphic, the bands ranged from 200 to 2000 bp. The
dendrogram grouped the accession into six clusters. The results indicate the
existence of high genetic distance values up to 0.8776 among the evaluated
accessions, even if the accessions were collected in the same country, or by
the contrary, lower genetic diversity among accessions collected in
different countries. It may be due to the existence of five ancient races of
sorghum, from which were originated most of the wild and cultivated species
known nowadays. Mainly, the migration of people from the origin centre of
sorghum, located in Ethiopia and Sudan, explain the spread of the genetic
material out of Africa. The information generated by this study should be
useful for a better understanding of the genetic diversity from the sorghum
germplasm stored in the Czech Plant Gene Bank for future plant breeding
program. |
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ISSN: | 0534-0012 1820-6069 |