Neuroprotective effects of mild hypoxia in organotypic hippocampal slice cultures
PurposeThe aim of this study was to investigate the potential effects of mild hypoxia in the mature and immature brain.MethodsWe prepared organotypic slice cultures of the hippocampus and used hippocampal tissue cultures at 7 and 14 days in vitro (DIV) to represent the immature and mature brain, res...
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Korean Pediatric Society
2015-04-01
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doaj-f769b60790764589b0df82d4979bebb42020-11-25T01:10:09ZengKorean Pediatric SocietyKorean Journal of Pediatrics1738-10612092-72582015-04-0158414214710.3345/kjp.2015.58.4.1422014600058Neuroprotective effects of mild hypoxia in organotypic hippocampal slice culturesSeh Hyun Kim0Woo Soon Lee1Na Mi Lee2Soo Ahn Chae3Sin Weon Yun4Department of Pediatrics, Chung-Ang University College of Medicine, Seoul, Korea.Department of Pediatrics, Chung-Ang University College of Medicine, Seoul, Korea.Department of Pediatrics, Chung-Ang University College of Medicine, Seoul, Korea.Department of Pediatrics, Chung-Ang University College of Medicine, Seoul, Korea.Department of Pediatrics, Chung-Ang University College of Medicine, Seoul, Korea.PurposeThe aim of this study was to investigate the potential effects of mild hypoxia in the mature and immature brain.MethodsWe prepared organotypic slice cultures of the hippocampus and used hippocampal tissue cultures at 7 and 14 days in vitro (DIV) to represent the immature and mature brain, respectively. Tissue cultures were exposed to 10% oxygen for 60 minutes. Twenty-four hours after this hypoxic insult, propidium iodide fluorescence images were obtained, and the damaged areas in the cornu ammonis 1 (CA1), CA3, and dentate gyrus (DG) were measured using image analysis.ResultsIn the 7-DIV group compared to control tissue, hypoxia-exposed tissue showed decreased damage in two regions (CA1: 5.59%±2.99% vs. 4.80%±1.37%, P=0.900; DG: 33.88%±12.53% vs. 15.98%±2.37%, P=0.166), but this decrease was not statistically significant. In the 14-DIV group, hypoxia-exposed tissue showed decreased damage compared to control tissues; this decrease was not significant in the CA3 (24.51%±6.05% vs. 18.31%±3.28%, P=0.373) or DG (15.72%±3.47% vs. 9.91%±2.11%, P=0.134), but was significant in the CA1 (50.91%±5.90% vs. 32.30%±3.34%, P=0.004).ConclusionAlthough only CA1 tissues cultured for 14 DIV showed significantly less damage after exposure to hypoxia, the other tissues examined in this study showed a tendency towards less damage after hypoxic exposure. Therefore, mild hypoxia might play a protective role in the brain.http://kjp.or.kr/upload/pdf/kjped-58-142.pdfHippocampusHypoxiaBrain |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Seh Hyun Kim Woo Soon Lee Na Mi Lee Soo Ahn Chae Sin Weon Yun |
spellingShingle |
Seh Hyun Kim Woo Soon Lee Na Mi Lee Soo Ahn Chae Sin Weon Yun Neuroprotective effects of mild hypoxia in organotypic hippocampal slice cultures Korean Journal of Pediatrics Hippocampus Hypoxia Brain |
author_facet |
Seh Hyun Kim Woo Soon Lee Na Mi Lee Soo Ahn Chae Sin Weon Yun |
author_sort |
Seh Hyun Kim |
title |
Neuroprotective effects of mild hypoxia in organotypic hippocampal slice cultures |
title_short |
Neuroprotective effects of mild hypoxia in organotypic hippocampal slice cultures |
title_full |
Neuroprotective effects of mild hypoxia in organotypic hippocampal slice cultures |
title_fullStr |
Neuroprotective effects of mild hypoxia in organotypic hippocampal slice cultures |
title_full_unstemmed |
Neuroprotective effects of mild hypoxia in organotypic hippocampal slice cultures |
title_sort |
neuroprotective effects of mild hypoxia in organotypic hippocampal slice cultures |
publisher |
Korean Pediatric Society |
series |
Korean Journal of Pediatrics |
issn |
1738-1061 2092-7258 |
publishDate |
2015-04-01 |
description |
PurposeThe aim of this study was to investigate the potential effects of mild hypoxia in the mature and immature brain.MethodsWe prepared organotypic slice cultures of the hippocampus and used hippocampal tissue cultures at 7 and 14 days in vitro (DIV) to represent the immature and mature brain, respectively. Tissue cultures were exposed to 10% oxygen for 60 minutes. Twenty-four hours after this hypoxic insult, propidium iodide fluorescence images were obtained, and the damaged areas in the cornu ammonis 1 (CA1), CA3, and dentate gyrus (DG) were measured using image analysis.ResultsIn the 7-DIV group compared to control tissue, hypoxia-exposed tissue showed decreased damage in two regions (CA1: 5.59%±2.99% vs. 4.80%±1.37%, P=0.900; DG: 33.88%±12.53% vs. 15.98%±2.37%, P=0.166), but this decrease was not statistically significant. In the 14-DIV group, hypoxia-exposed tissue showed decreased damage compared to control tissues; this decrease was not significant in the CA3 (24.51%±6.05% vs. 18.31%±3.28%, P=0.373) or DG (15.72%±3.47% vs. 9.91%±2.11%, P=0.134), but was significant in the CA1 (50.91%±5.90% vs. 32.30%±3.34%, P=0.004).ConclusionAlthough only CA1 tissues cultured for 14 DIV showed significantly less damage after exposure to hypoxia, the other tissues examined in this study showed a tendency towards less damage after hypoxic exposure. Therefore, mild hypoxia might play a protective role in the brain. |
topic |
Hippocampus Hypoxia Brain |
url |
http://kjp.or.kr/upload/pdf/kjped-58-142.pdf |
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