Far-field unlabeled super-resolution imaging with superoscillatory illumination
Unlabeled super-resolution is the next grand challenge in imaging. Stimulated emission depletion and single-molecule microscopies have revolutionized the life sciences but are still limited by the need for reporters (labels) embedded within the sample. While the Veselago–Pendry “super-lens,” using a...
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2020-06-01
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Online Access: | http://dx.doi.org/10.1063/1.5144918 |
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doaj-f7761f5d08704db9bdfbf0e9594be5e72020-11-25T04:09:07ZengAIP Publishing LLCAPL Photonics2378-09672020-06-0156066107066107-1010.1063/1.5144918Far-field unlabeled super-resolution imaging with superoscillatory illuminationEdward T. F. Rogers0Shmma Quraishe1Katrine S. Rogers2Tracey A. Newman3Peter J. S. Smith4Nikolay I. Zheludev5Institute for Life Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United KingdomClinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton SO17 1BJ, United KingdomSchool of Mathematics and Statistics, The Open University, Walton Hall, Milton Keynes MK7 6AA, United KingdomClinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton SO17 1BJ, United KingdomInstitute for Life Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, United KingdomOptoelectronics Research Centre and Centre for Photonic Metamaterials, University of Southampton, Highfield, Southampton SO17 1BJ, United KingdomUnlabeled super-resolution is the next grand challenge in imaging. Stimulated emission depletion and single-molecule microscopies have revolutionized the life sciences but are still limited by the need for reporters (labels) embedded within the sample. While the Veselago–Pendry “super-lens,” using a negative-index metamaterial, is a promising idea for imaging beyond the diffraction limit, there are substantial technological challenges to its realization. Another route to far-field subwavelength focusing is using optical superoscillations: engineered interference of multiple coherent waves creating an, in principle, arbitrarily small hotspot. Here, we demonstrate microscopy with superoscillatory illumination of the object and describe its underlying principles. We show that far-field images taken with superoscillatory illumination are themselves superoscillatory and, hence, can reveal fine structural details of the object that are lost in conventional far-field imaging. We show that the resolution of a superoscillatory microscope is determined by the size of the hotspot, rather than the bandwidth of the optical instrument. We demonstrate high-frame-rate polarization-contrast imaging of unmodified living cells with a resolution significantly exceeding that achievable with conventional instruments. This non-algorithmic, low-phototoxicity imaging technology is a powerful tool both for biological research and for super-resolution imaging of samples that do not allow labeling, such as the interior of silicon chips.http://dx.doi.org/10.1063/1.5144918 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Edward T. F. Rogers Shmma Quraishe Katrine S. Rogers Tracey A. Newman Peter J. S. Smith Nikolay I. Zheludev |
spellingShingle |
Edward T. F. Rogers Shmma Quraishe Katrine S. Rogers Tracey A. Newman Peter J. S. Smith Nikolay I. Zheludev Far-field unlabeled super-resolution imaging with superoscillatory illumination APL Photonics |
author_facet |
Edward T. F. Rogers Shmma Quraishe Katrine S. Rogers Tracey A. Newman Peter J. S. Smith Nikolay I. Zheludev |
author_sort |
Edward T. F. Rogers |
title |
Far-field unlabeled super-resolution imaging with superoscillatory illumination |
title_short |
Far-field unlabeled super-resolution imaging with superoscillatory illumination |
title_full |
Far-field unlabeled super-resolution imaging with superoscillatory illumination |
title_fullStr |
Far-field unlabeled super-resolution imaging with superoscillatory illumination |
title_full_unstemmed |
Far-field unlabeled super-resolution imaging with superoscillatory illumination |
title_sort |
far-field unlabeled super-resolution imaging with superoscillatory illumination |
publisher |
AIP Publishing LLC |
series |
APL Photonics |
issn |
2378-0967 |
publishDate |
2020-06-01 |
description |
Unlabeled super-resolution is the next grand challenge in imaging. Stimulated emission depletion and single-molecule microscopies have revolutionized the life sciences but are still limited by the need for reporters (labels) embedded within the sample. While the Veselago–Pendry “super-lens,” using a negative-index metamaterial, is a promising idea for imaging beyond the diffraction limit, there are substantial technological challenges to its realization. Another route to far-field subwavelength focusing is using optical superoscillations: engineered interference of multiple coherent waves creating an, in principle, arbitrarily small hotspot. Here, we demonstrate microscopy with superoscillatory illumination of the object and describe its underlying principles. We show that far-field images taken with superoscillatory illumination are themselves superoscillatory and, hence, can reveal fine structural details of the object that are lost in conventional far-field imaging. We show that the resolution of a superoscillatory microscope is determined by the size of the hotspot, rather than the bandwidth of the optical instrument. We demonstrate high-frame-rate polarization-contrast imaging of unmodified living cells with a resolution significantly exceeding that achievable with conventional instruments. This non-algorithmic, low-phototoxicity imaging technology is a powerful tool both for biological research and for super-resolution imaging of samples that do not allow labeling, such as the interior of silicon chips. |
url |
http://dx.doi.org/10.1063/1.5144918 |
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