Single-cell analysis of patient-derived PDAC organoids reveals cell state heterogeneity and a conserved developmental hierarchy

Pancreatic tumors are frequently divided into basal and classical subtypes. Here, the authors use single cell sequencing to investigate organoids derived from pancreatic cancer tissue and find a hierarchy of distinct cell states, and classical and basal cells existing within the same tumor.

Bibliographic Details
Main Authors:	Teresa G. Krieger, Solange Le Blanc, Julia Jabs, Foo Wei Ten, Naveed Ishaque, Katharina Jechow, Olivia Debnath, Carl-Stephan Leonhardt, Anamika Giri, Roland Eils, Oliver Strobel, Christian Conrad
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-10-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-021-26059-4

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spelling	doaj-f77b80eb08eb4c0a93db5169bb083e642021-10-10T11:45:28ZengNature Publishing GroupNature Communications2041-17232021-10-0112111310.1038/s41467-021-26059-4Single-cell analysis of patient-derived PDAC organoids reveals cell state heterogeneity and a conserved developmental hierarchyTeresa G. Krieger0Solange Le Blanc1Julia Jabs2Foo Wei Ten3Naveed Ishaque4Katharina Jechow5Olivia Debnath6Carl-Stephan Leonhardt7Anamika Giri8Roland Eils9Oliver Strobel10Christian Conrad11Digital Health Center, Berlin Institute of Health (BIH)/Charité-Universitätsmedizin BerlinEuropean Pancreas Center, Department of General Surgery, Heidelberg University HospitalDivision of Theoretical Bioinformatics, German Cancer Research Center (DKFZ)Digital Health Center, Berlin Institute of Health (BIH)/Charité-Universitätsmedizin BerlinDigital Health Center, Berlin Institute of Health (BIH)/Charité-Universitätsmedizin BerlinDigital Health Center, Berlin Institute of Health (BIH)/Charité-Universitätsmedizin BerlinDigital Health Center, Berlin Institute of Health (BIH)/Charité-Universitätsmedizin BerlinEuropean Pancreas Center, Department of General Surgery, Heidelberg University HospitalDivision of Theoretical Bioinformatics, German Cancer Research Center (DKFZ)Digital Health Center, Berlin Institute of Health (BIH)/Charité-Universitätsmedizin BerlinEuropean Pancreas Center, Department of General Surgery, Heidelberg University HospitalDigital Health Center, Berlin Institute of Health (BIH)/Charité-Universitätsmedizin BerlinPancreatic tumors are frequently divided into basal and classical subtypes. Here, the authors use single cell sequencing to investigate organoids derived from pancreatic cancer tissue and find a hierarchy of distinct cell states, and classical and basal cells existing within the same tumor.https://doi.org/10.1038/s41467-021-26059-4
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Teresa G. Krieger Solange Le Blanc Julia Jabs Foo Wei Ten Naveed Ishaque Katharina Jechow Olivia Debnath Carl-Stephan Leonhardt Anamika Giri Roland Eils Oliver Strobel Christian Conrad
spellingShingle	Teresa G. Krieger Solange Le Blanc Julia Jabs Foo Wei Ten Naveed Ishaque Katharina Jechow Olivia Debnath Carl-Stephan Leonhardt Anamika Giri Roland Eils Oliver Strobel Christian Conrad Single-cell analysis of patient-derived PDAC organoids reveals cell state heterogeneity and a conserved developmental hierarchy Nature Communications
author_facet	Teresa G. Krieger Solange Le Blanc Julia Jabs Foo Wei Ten Naveed Ishaque Katharina Jechow Olivia Debnath Carl-Stephan Leonhardt Anamika Giri Roland Eils Oliver Strobel Christian Conrad
author_sort	Teresa G. Krieger
title	Single-cell analysis of patient-derived PDAC organoids reveals cell state heterogeneity and a conserved developmental hierarchy
title_short	Single-cell analysis of patient-derived PDAC organoids reveals cell state heterogeneity and a conserved developmental hierarchy
title_full	Single-cell analysis of patient-derived PDAC organoids reveals cell state heterogeneity and a conserved developmental hierarchy
title_fullStr	Single-cell analysis of patient-derived PDAC organoids reveals cell state heterogeneity and a conserved developmental hierarchy
title_full_unstemmed	Single-cell analysis of patient-derived PDAC organoids reveals cell state heterogeneity and a conserved developmental hierarchy
title_sort	single-cell analysis of patient-derived pdac organoids reveals cell state heterogeneity and a conserved developmental hierarchy
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2021-10-01
description	Pancreatic tumors are frequently divided into basal and classical subtypes. Here, the authors use single cell sequencing to investigate organoids derived from pancreatic cancer tissue and find a hierarchy of distinct cell states, and classical and basal cells existing within the same tumor.
url	https://doi.org/10.1038/s41467-021-26059-4
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Single-cell analysis of patient-derived PDAC organoids reveals cell state heterogeneity and a conserved developmental hierarchy

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