Low efficiency of homology-facilitated illegitimate recombination during conjugation in Escherichia coli.
Homology-facilitated illegitimate recombination has been described in three naturally competent bacterial species. It permits integration of small linear DNA molecules into the chromosome by homologous recombination at one end of the linear DNA substrate, and illegitimate recombination at the other...
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2011-01-01
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doaj-f7969b006f7544fdb332b4ba8ed44b6e2020-11-25T01:16:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01612e2887610.1371/journal.pone.0028876Low efficiency of homology-facilitated illegitimate recombination during conjugation in Escherichia coli.Jihane Amarir-BouhramMélodie GoinMarie-Agnès PetitHomology-facilitated illegitimate recombination has been described in three naturally competent bacterial species. It permits integration of small linear DNA molecules into the chromosome by homologous recombination at one end of the linear DNA substrate, and illegitimate recombination at the other end. We report that homology-facilitated illegitimate recombination also occurs in Escherichia coli during conjugation with small non-replicative plasmids, but at a low frequency of 3×10(-10) per recipient cell. The fate of linear DNA in E. coli is either RecBCD-dependent degradation, or circularisation by ligation, and integration into the chromosome by single crossing-over. We also report that the observed single crossing-overs are recA-dependent, but essentially recBCD, and recFOR independent. This suggests that other, still unknown, proteins may act as mediator for the loading of RecA on DNA during single crossing-over recombination in E. coli.http://europepmc.org/articles/PMC3240628?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jihane Amarir-Bouhram Mélodie Goin Marie-Agnès Petit |
spellingShingle |
Jihane Amarir-Bouhram Mélodie Goin Marie-Agnès Petit Low efficiency of homology-facilitated illegitimate recombination during conjugation in Escherichia coli. PLoS ONE |
author_facet |
Jihane Amarir-Bouhram Mélodie Goin Marie-Agnès Petit |
author_sort |
Jihane Amarir-Bouhram |
title |
Low efficiency of homology-facilitated illegitimate recombination during conjugation in Escherichia coli. |
title_short |
Low efficiency of homology-facilitated illegitimate recombination during conjugation in Escherichia coli. |
title_full |
Low efficiency of homology-facilitated illegitimate recombination during conjugation in Escherichia coli. |
title_fullStr |
Low efficiency of homology-facilitated illegitimate recombination during conjugation in Escherichia coli. |
title_full_unstemmed |
Low efficiency of homology-facilitated illegitimate recombination during conjugation in Escherichia coli. |
title_sort |
low efficiency of homology-facilitated illegitimate recombination during conjugation in escherichia coli. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-01-01 |
description |
Homology-facilitated illegitimate recombination has been described in three naturally competent bacterial species. It permits integration of small linear DNA molecules into the chromosome by homologous recombination at one end of the linear DNA substrate, and illegitimate recombination at the other end. We report that homology-facilitated illegitimate recombination also occurs in Escherichia coli during conjugation with small non-replicative plasmids, but at a low frequency of 3×10(-10) per recipient cell. The fate of linear DNA in E. coli is either RecBCD-dependent degradation, or circularisation by ligation, and integration into the chromosome by single crossing-over. We also report that the observed single crossing-overs are recA-dependent, but essentially recBCD, and recFOR independent. This suggests that other, still unknown, proteins may act as mediator for the loading of RecA on DNA during single crossing-over recombination in E. coli. |
url |
http://europepmc.org/articles/PMC3240628?pdf=render |
work_keys_str_mv |
AT jihaneamarirbouhram lowefficiencyofhomologyfacilitatedillegitimaterecombinationduringconjugationinescherichiacoli AT melodiegoin lowefficiencyofhomologyfacilitatedillegitimaterecombinationduringconjugationinescherichiacoli AT marieagnespetit lowefficiencyofhomologyfacilitatedillegitimaterecombinationduringconjugationinescherichiacoli |
_version_ |
1725149158149455872 |