β-hydroxy-β-methyl butyrate, but not α-ketoisocaproate and excess leucine, stimulates skeletal muscle protein metabolism in growing pigs fed low-protein diets
This study aimed to investigate the effects of leucine (Leu) and its metabolites (α-ketoisocaproate (KIC) and β-hydroxy-β-methylbutyrate (HMB)) on muscle protein metabolism in growing pigs fed low-protein diets. 32 pigs (Large White × Landrace, 9.55 ± 0.19 kg) were divided into four groups (basal di...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-01-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464618305486 |
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doaj-f7a793ffc67a43f3b61a2434536fdd57 |
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Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Yehui Duan Fengna Li Bo Song Changbing Zheng Yinzhao Zhong Kang Xu Xiangfeng Kong Yulong Yin Wence Wang Gang Shu |
| spellingShingle |
Yehui Duan Fengna Li Bo Song Changbing Zheng Yinzhao Zhong Kang Xu Xiangfeng Kong Yulong Yin Wence Wang Gang Shu β-hydroxy-β-methyl butyrate, but not α-ketoisocaproate and excess leucine, stimulates skeletal muscle protein metabolism in growing pigs fed low-protein diets Journal of Functional Foods β-hydroxy-β-methyl butyrate Protein synthesis Protein degradation Akt Skeletal muscle Growing pigs |
| author_facet |
Yehui Duan Fengna Li Bo Song Changbing Zheng Yinzhao Zhong Kang Xu Xiangfeng Kong Yulong Yin Wence Wang Gang Shu |
| author_sort |
Yehui Duan |
| title |
β-hydroxy-β-methyl butyrate, but not α-ketoisocaproate and excess leucine, stimulates skeletal muscle protein metabolism in growing pigs fed low-protein diets |
| title_short |
β-hydroxy-β-methyl butyrate, but not α-ketoisocaproate and excess leucine, stimulates skeletal muscle protein metabolism in growing pigs fed low-protein diets |
| title_full |
β-hydroxy-β-methyl butyrate, but not α-ketoisocaproate and excess leucine, stimulates skeletal muscle protein metabolism in growing pigs fed low-protein diets |
| title_fullStr |
β-hydroxy-β-methyl butyrate, but not α-ketoisocaproate and excess leucine, stimulates skeletal muscle protein metabolism in growing pigs fed low-protein diets |
| title_full_unstemmed |
β-hydroxy-β-methyl butyrate, but not α-ketoisocaproate and excess leucine, stimulates skeletal muscle protein metabolism in growing pigs fed low-protein diets |
| title_sort |
β-hydroxy-β-methyl butyrate, but not α-ketoisocaproate and excess leucine, stimulates skeletal muscle protein metabolism in growing pigs fed low-protein diets |
| publisher |
Elsevier |
| series |
Journal of Functional Foods |
| issn |
1756-4646 |
| publishDate |
2019-01-01 |
| description |
This study aimed to investigate the effects of leucine (Leu) and its metabolites (α-ketoisocaproate (KIC) and β-hydroxy-β-methylbutyrate (HMB)) on muscle protein metabolism in growing pigs fed low-protein diets. 32 pigs (Large White × Landrace, 9.55 ± 0.19 kg) were divided into four groups (basal diet, L-Leu, KIC-Ca, HMB-Ca). Results showed that compared to the control, dietary supplementation of KIC and excess Leu significantly decreased the weight of soleus muscle (SM, P < 0.05), whereas HMB supplementation increased it, accompanied by elevated rate of protein synthesis and elevated phosphorylation of Akt (P < 0.05). The weight of longissimus dorsi muscle remained unaffected by dietary treatments (P > 0.05). In conclusion, HMB, but not KIC or excess Leu, might act as a nutrient signal to stimulate positive protein balance especially in muscles of oxidative fiber-types of growing pigs fed low-protein diets, and these effects might be associated with the Akt signaling. |
| topic |
β-hydroxy-β-methyl butyrate Protein synthesis Protein degradation Akt Skeletal muscle Growing pigs |
| url |
http://www.sciencedirect.com/science/article/pii/S1756464618305486 |
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doaj-f7a793ffc67a43f3b61a2434536fdd572021-04-30T07:14:57ZengElsevierJournal of Functional Foods1756-46462019-01-01523442β-hydroxy-β-methyl butyrate, but not α-ketoisocaproate and excess leucine, stimulates skeletal muscle protein metabolism in growing pigs fed low-protein dietsYehui Duan0Fengna Li1Bo Song2Changbing Zheng3Yinzhao Zhong4Kang Xu5Xiangfeng Kong6Yulong Yin7Wence Wang8Gang Shu9Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha 410125, ChinaHunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha 410125, China; Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Changsha, Hunan, China; Corresponding authors at: Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China (F. Li). South China Agricultural University, Guangzhou 510642, China (G. Shu).Guangdong Provincial Key Laboratory of Animal Nutrition Regulation, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Regulation, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Regulation, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaHunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha 410125, ChinaHunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha 410125, ChinaHunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha 410125, China; Laboratory of Animal Nutrition and Human Health, School of Biology, Hunan Normal University, Changsha Hunan 410018, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Regulation, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Regulation, South China Agricultural University, Guangzhou, Guangdong 510642, China; Corresponding authors at: Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China (F. Li). South China Agricultural University, Guangzhou 510642, China (G. Shu).This study aimed to investigate the effects of leucine (Leu) and its metabolites (α-ketoisocaproate (KIC) and β-hydroxy-β-methylbutyrate (HMB)) on muscle protein metabolism in growing pigs fed low-protein diets. 32 pigs (Large White × Landrace, 9.55 ± 0.19 kg) were divided into four groups (basal diet, L-Leu, KIC-Ca, HMB-Ca). Results showed that compared to the control, dietary supplementation of KIC and excess Leu significantly decreased the weight of soleus muscle (SM, P < 0.05), whereas HMB supplementation increased it, accompanied by elevated rate of protein synthesis and elevated phosphorylation of Akt (P < 0.05). The weight of longissimus dorsi muscle remained unaffected by dietary treatments (P > 0.05). In conclusion, HMB, but not KIC or excess Leu, might act as a nutrient signal to stimulate positive protein balance especially in muscles of oxidative fiber-types of growing pigs fed low-protein diets, and these effects might be associated with the Akt signaling.http://www.sciencedirect.com/science/article/pii/S1756464618305486β-hydroxy-β-methyl butyrateProtein synthesisProtein degradationAktSkeletal muscleGrowing pigs |