Suppressive effect of TNF-α and IL-1 on alveolar fibroblast proliferation in sarcoidosis
The nature of soluble factors that regulate fibroblast proliferation have not been finally characterized. Our aim was to study the role of tumour necrosis factor α (TNF-α) and interleukin-1 (IL-1) in the suppressive activity of alveolar macrophages on autologous lung fibroblasts proliferation in sar...
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Hindawi Limited
1992-01-01
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Series: | Mediators of Inflammation |
Online Access: | http://dx.doi.org/10.1155/S0962935192000474 |
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doaj-f7b5af5693ae459ea41a4e7e6b32aa832020-11-24T23:40:05ZengHindawi LimitedMediators of Inflammation0962-93511466-18611992-01-011531932210.1155/S0962935192000474Suppressive effect of TNF-α and IL-1 on alveolar fibroblast proliferation in sarcoidosisE. Fireman0D. Aderka1S. Ben Efraim2J. Greif3D. Wallach4M. Topilsky5Department of Pulmonary Diseases and Allergic Diseases, TeI-Aviv Medical Center, Ichilov Hospital, 6 Weizmann Street, TeI-Aviv 64268, IsraelDepartment of Internal Medicine “T”, Ichilov Hospital, 6 Weizmann Street, TeI-Aviv 64239, IsraelDepartment of Human Microbiology, Sackler School of Medicine, TeI-Aviv University, Ramat Aviv, TeI-Aviv 69978, IsraelDepartment of Pulmonary Diseases and Allergic Diseases, TeI-Aviv Medical Center, Ichilov Hospital, 6 Weizmann Street, TeI-Aviv 64268, IsraelDepartment of Virology, Weizmann Institute of Science, Rehovot 76326, IsraelDepartment of Pulmonary Diseases and Allergic Diseases, TeI-Aviv Medical Center, Ichilov Hospital, 6 Weizmann Street, TeI-Aviv 64268, IsraelThe nature of soluble factors that regulate fibroblast proliferation have not been finally characterized. Our aim was to study the role of tumour necrosis factor α (TNF-α) and interleukin-1 (IL-1) in the suppressive activity of alveolar macrophages on autologous lung fibroblasts proliferation in sarcoidosis. We found that supernatants recovered from alveolar macrophages suppressed the proliferation of alveolar fibroblast in sarcoidosis by 35.5 ± 1.13% compared to 3 ± 16% in controls (p < 0.001 between the two groups). This suppression correlated with high content of TNF-α and IL-1 in sarcoidosis patients stage II-III (7.7 ± 2.9 ng/ml TNF-α and 157 ± 53 U/ml IL-1 compared to 3.4 ± 2.4 ng/ml TNF-α and 43 U/ml IL-1 in controls; p < 0.01 and p < 0.001, respectively). Both cytokines in sarcoidosis stage I were within the normal ranges. Exogenous TNF-α (1000-0.5 ng/ml) and IL-1 (500-0.24 ng/ml) had an additive suppressive activity on fibroblast proliferation which was partially reversed by indomethacin.http://dx.doi.org/10.1155/S0962935192000474 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
E. Fireman D. Aderka S. Ben Efraim J. Greif D. Wallach M. Topilsky |
spellingShingle |
E. Fireman D. Aderka S. Ben Efraim J. Greif D. Wallach M. Topilsky Suppressive effect of TNF-α and IL-1 on alveolar fibroblast proliferation in sarcoidosis Mediators of Inflammation |
author_facet |
E. Fireman D. Aderka S. Ben Efraim J. Greif D. Wallach M. Topilsky |
author_sort |
E. Fireman |
title |
Suppressive effect of TNF-α and IL-1 on alveolar fibroblast proliferation in sarcoidosis |
title_short |
Suppressive effect of TNF-α and IL-1 on alveolar fibroblast proliferation in sarcoidosis |
title_full |
Suppressive effect of TNF-α and IL-1 on alveolar fibroblast proliferation in sarcoidosis |
title_fullStr |
Suppressive effect of TNF-α and IL-1 on alveolar fibroblast proliferation in sarcoidosis |
title_full_unstemmed |
Suppressive effect of TNF-α and IL-1 on alveolar fibroblast proliferation in sarcoidosis |
title_sort |
suppressive effect of tnf-α and il-1 on alveolar fibroblast proliferation in sarcoidosis |
publisher |
Hindawi Limited |
series |
Mediators of Inflammation |
issn |
0962-9351 1466-1861 |
publishDate |
1992-01-01 |
description |
The nature of soluble factors that regulate fibroblast proliferation have not been finally characterized. Our aim was to study the role of tumour necrosis factor α (TNF-α) and interleukin-1 (IL-1) in the suppressive activity of alveolar macrophages on autologous lung fibroblasts proliferation in sarcoidosis. We found that supernatants recovered from alveolar macrophages suppressed the proliferation of alveolar fibroblast in sarcoidosis by 35.5 ± 1.13% compared to 3 ± 16% in controls (p < 0.001 between the two groups). This suppression correlated with high content of TNF-α and IL-1 in sarcoidosis patients stage II-III (7.7 ± 2.9 ng/ml TNF-α and 157 ± 53 U/ml IL-1 compared to 3.4 ± 2.4 ng/ml TNF-α and 43 U/ml IL-1 in controls; p < 0.01 and p < 0.001, respectively). Both cytokines in sarcoidosis stage I were within the normal ranges. Exogenous TNF-α (1000-0.5 ng/ml) and IL-1 (500-0.24 ng/ml) had an additive suppressive activity on fibroblast proliferation which was partially reversed by indomethacin. |
url |
http://dx.doi.org/10.1155/S0962935192000474 |
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