High-Sensitivity in Dielectrophoresis Separations
The applications of dielectrophoretic (DEP) techniques for the manipulation of cells in a label-free fashion within microfluidic systems continue to grow. However, a limited number of methods exist for making highly sensitive separations that can isolate subtle phenotypic differences within a popula...
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MDPI AG
2020-04-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/11/4/391 |
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doaj-f7bad0120982492ba3dad333db9995902020-11-25T03:37:14ZengMDPI AGMicromachines2072-666X2020-04-011139139110.3390/mi11040391High-Sensitivity in Dielectrophoresis SeparationsBenjamin G. Hawkins0Nelson Lai1David S. Clague2Biomedical Engineering Department, California Polytechnic State University, San Luis Obispo, CA 93407, USABiomedical Engineering Department, California Polytechnic State University, San Luis Obispo, CA 93407, USABiomedical Engineering Department, California Polytechnic State University, San Luis Obispo, CA 93407, USAThe applications of dielectrophoretic (DEP) techniques for the manipulation of cells in a label-free fashion within microfluidic systems continue to grow. However, a limited number of methods exist for making highly sensitive separations that can isolate subtle phenotypic differences within a population of cells. This paper explores efforts to leverage that most compelling aspect of DEP—an actuation force that depends on particle electrical properties—in the background of phenotypic variations in cell size. Several promising approaches, centering around the application of multiple electric fields with spatially mapped magnitude and/or frequencies, are expanding the capability of DEP cell separation.https://www.mdpi.com/2072-666X/11/4/391dielectrophoresismicrofluidicscell separation |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Benjamin G. Hawkins Nelson Lai David S. Clague |
| spellingShingle |
Benjamin G. Hawkins Nelson Lai David S. Clague High-Sensitivity in Dielectrophoresis Separations Micromachines dielectrophoresis microfluidics cell separation |
| author_facet |
Benjamin G. Hawkins Nelson Lai David S. Clague |
| author_sort |
Benjamin G. Hawkins |
| title |
High-Sensitivity in Dielectrophoresis Separations |
| title_short |
High-Sensitivity in Dielectrophoresis Separations |
| title_full |
High-Sensitivity in Dielectrophoresis Separations |
| title_fullStr |
High-Sensitivity in Dielectrophoresis Separations |
| title_full_unstemmed |
High-Sensitivity in Dielectrophoresis Separations |
| title_sort |
high-sensitivity in dielectrophoresis separations |
| publisher |
MDPI AG |
| series |
Micromachines |
| issn |
2072-666X |
| publishDate |
2020-04-01 |
| description |
The applications of dielectrophoretic (DEP) techniques for the manipulation of cells in a label-free fashion within microfluidic systems continue to grow. However, a limited number of methods exist for making highly sensitive separations that can isolate subtle phenotypic differences within a population of cells. This paper explores efforts to leverage that most compelling aspect of DEP—an actuation force that depends on particle electrical properties—in the background of phenotypic variations in cell size. Several promising approaches, centering around the application of multiple electric fields with spatially mapped magnitude and/or frequencies, are expanding the capability of DEP cell separation. |
| topic |
dielectrophoresis microfluidics cell separation |
| url |
https://www.mdpi.com/2072-666X/11/4/391 |
| work_keys_str_mv |
AT benjaminghawkins highsensitivityindielectrophoresisseparations AT nelsonlai highsensitivityindielectrophoresisseparations AT davidsclague highsensitivityindielectrophoresisseparations |
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