The effect of propofol and sevoflurane on cancer cell, natural killer cell, and cytotoxic T lymphocyte function in patients undergoing breast cancer surgery: an in vitro analysis
Abstract Background To clarify the effect of anaesthetic agents on cancer immunity, we evaluated the effects of propofol and sevoflurane on natural killer (NK) cell, cytotoxic T lymphocyte (CTL) counts and apoptosis rate in breast cancer and immune cells co-cultures from patients who underwent breas...
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doaj-f7be82819af2476cae1e912ef744280d2020-11-24T21:21:42ZengBMCBMC Cancer1471-24072018-02-011811810.1186/s12885-018-4064-8The effect of propofol and sevoflurane on cancer cell, natural killer cell, and cytotoxic T lymphocyte function in patients undergoing breast cancer surgery: an in vitro analysisJeong-Ae Lim0Chung-Sik Oh1Tae-Gyoon Yoon2Ji Yeon Lee3Seung-Hyun Lee4Young-Bum Yoo5Jung-Hyun Yang6Seong-Hyop Kim7Department of Anaesthesiology and Pain medicine, Konkuk University Medical Centre, Konkuk University School of MedicineDepartment of Anaesthesiology and Pain medicine, Konkuk University Medical Centre, Konkuk University School of MedicineDepartment of Anaesthesiology and Pain medicine, Konkuk University Medical Centre, Konkuk University School of MedicineDepartment of Microbiology, Konkuk University School of MedicineDepartment of Microbiology, Konkuk University School of MedicineDepartment of Surgery, Konkuk University Medical Centre, Konkuk University School of MedicineDepartment of Surgery, Konkuk University Medical Centre, Konkuk University School of MedicineDepartment of Anaesthesiology and Pain medicine, Konkuk University Medical Centre, Konkuk University School of MedicineAbstract Background To clarify the effect of anaesthetic agents on cancer immunity, we evaluated the effects of propofol and sevoflurane on natural killer (NK) cell, cytotoxic T lymphocyte (CTL) counts and apoptosis rate in breast cancer and immune cells co-cultures from patients who underwent breast cancer surgery. Methods Venous blood samples were collected after inducing anaesthesia and at 1 and 24 h postoperatively in patients who had undergone breast cancer surgery. The patients were allocated randomly to the propofol- or sevoflurane-based anaesthesia groups. We counted and detected apoptosis in cancer cell, NK cell and CTL of patients with breast cancer by co-culture with a breast cancer cell line in both groups. We also evaluated changes in the cytokines tumour necrosis factor-alpha, interleukin (IL)-6 and IL-10 during the perioperative period. Results Forty-four patients were included in the final analysis. No difference in NK cell count, CTL count or apoptosis rate was detected between the groups. Furthermore, the number of breast cancer cells undergoing apoptosis in the breast cancer cell co-cultures was not different between the groups. No changes in cytokines were detected between the groups. Conclusion Although basic science studies have suggested the potential benefits of propofol over a volatile agent during cancer surgery, propofol was not superior to sevoflurane, on the aspects of NK and CTL cells counts with apoptosis rate including breast cancer cell, during anaesthesia for breast cancer surgery in a clinical environment. Trial registration NCT02758249 on February 26, 2016.http://link.springer.com/article/10.1186/s12885-018-4064-8Breast cancerPropofolSevofluraneNatural killer cellCytotoxic T lymphocyte |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jeong-Ae Lim Chung-Sik Oh Tae-Gyoon Yoon Ji Yeon Lee Seung-Hyun Lee Young-Bum Yoo Jung-Hyun Yang Seong-Hyop Kim |
spellingShingle |
Jeong-Ae Lim Chung-Sik Oh Tae-Gyoon Yoon Ji Yeon Lee Seung-Hyun Lee Young-Bum Yoo Jung-Hyun Yang Seong-Hyop Kim The effect of propofol and sevoflurane on cancer cell, natural killer cell, and cytotoxic T lymphocyte function in patients undergoing breast cancer surgery: an in vitro analysis BMC Cancer Breast cancer Propofol Sevoflurane Natural killer cell Cytotoxic T lymphocyte |
author_facet |
Jeong-Ae Lim Chung-Sik Oh Tae-Gyoon Yoon Ji Yeon Lee Seung-Hyun Lee Young-Bum Yoo Jung-Hyun Yang Seong-Hyop Kim |
author_sort |
Jeong-Ae Lim |
title |
The effect of propofol and sevoflurane on cancer cell, natural killer cell, and cytotoxic T lymphocyte function in patients undergoing breast cancer surgery: an in vitro analysis |
title_short |
The effect of propofol and sevoflurane on cancer cell, natural killer cell, and cytotoxic T lymphocyte function in patients undergoing breast cancer surgery: an in vitro analysis |
title_full |
The effect of propofol and sevoflurane on cancer cell, natural killer cell, and cytotoxic T lymphocyte function in patients undergoing breast cancer surgery: an in vitro analysis |
title_fullStr |
The effect of propofol and sevoflurane on cancer cell, natural killer cell, and cytotoxic T lymphocyte function in patients undergoing breast cancer surgery: an in vitro analysis |
title_full_unstemmed |
The effect of propofol and sevoflurane on cancer cell, natural killer cell, and cytotoxic T lymphocyte function in patients undergoing breast cancer surgery: an in vitro analysis |
title_sort |
effect of propofol and sevoflurane on cancer cell, natural killer cell, and cytotoxic t lymphocyte function in patients undergoing breast cancer surgery: an in vitro analysis |
publisher |
BMC |
series |
BMC Cancer |
issn |
1471-2407 |
publishDate |
2018-02-01 |
description |
Abstract Background To clarify the effect of anaesthetic agents on cancer immunity, we evaluated the effects of propofol and sevoflurane on natural killer (NK) cell, cytotoxic T lymphocyte (CTL) counts and apoptosis rate in breast cancer and immune cells co-cultures from patients who underwent breast cancer surgery. Methods Venous blood samples were collected after inducing anaesthesia and at 1 and 24 h postoperatively in patients who had undergone breast cancer surgery. The patients were allocated randomly to the propofol- or sevoflurane-based anaesthesia groups. We counted and detected apoptosis in cancer cell, NK cell and CTL of patients with breast cancer by co-culture with a breast cancer cell line in both groups. We also evaluated changes in the cytokines tumour necrosis factor-alpha, interleukin (IL)-6 and IL-10 during the perioperative period. Results Forty-four patients were included in the final analysis. No difference in NK cell count, CTL count or apoptosis rate was detected between the groups. Furthermore, the number of breast cancer cells undergoing apoptosis in the breast cancer cell co-cultures was not different between the groups. No changes in cytokines were detected between the groups. Conclusion Although basic science studies have suggested the potential benefits of propofol over a volatile agent during cancer surgery, propofol was not superior to sevoflurane, on the aspects of NK and CTL cells counts with apoptosis rate including breast cancer cell, during anaesthesia for breast cancer surgery in a clinical environment. Trial registration NCT02758249 on February 26, 2016. |
topic |
Breast cancer Propofol Sevoflurane Natural killer cell Cytotoxic T lymphocyte |
url |
http://link.springer.com/article/10.1186/s12885-018-4064-8 |
work_keys_str_mv |
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