Association of miRNA-499 rs3746444 A>G variants with adenocarcinoma of esophagogastric junction (AEG) risk and lymph node status
Weifeng Tang,1,* Yafeng Wang,2,* Huiwen Pan,1 Hao Qiu,3 Shuchen Chen41Department of Cardiothoracic Surgery, Affiliated People’s Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, People’s Republic of China; 2Department of Cardiology, The People’s Hospital of X...
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doaj-f7d6ceb3ac0a4b108f57dc009e98b6f52020-11-25T01:58:48ZengDove Medical PressOncoTargets and Therapy1178-69302019-08-01Volume 126245625247727Association of miRNA-499 rs3746444 A>G variants with adenocarcinoma of esophagogastric junction (AEG) risk and lymph node statusTang WWang YPan HQiu HChen SWeifeng Tang,1,* Yafeng Wang,2,* Huiwen Pan,1 Hao Qiu,3 Shuchen Chen41Department of Cardiothoracic Surgery, Affiliated People’s Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, People’s Republic of China; 2Department of Cardiology, The People’s Hospital of Xishuangbanna dai Autonomous Prefecture, Jinghong, Yunnan Province, People’s Republic of China; 3Department of Immunology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu Province, People’s Republic of China; 4Department of Thoracic Surgery, Fujian Medical University Union Hospital, Fuzhou, Fujian Province, People’s Republic of China*These authors contributed equally to this workBackground: MicroRNAs (miRNAs) miRNA-499 rs3746444 A>G polymorphism may be complicated in the susceptibility to cancer. However, the correlation of this polymorphism with adenocarcinoma of esophagogastric junction (AEG) was unknown.Patients and methods: A total of 1063 AEG patients and 1677 controls were included in this study to assess the association of miR-499 rs3746444 A>G with AEG risk. SNPscanTM genotyping assay was harnessed to obtain the genotypes of miRNA-499 rs3746444 A>G polymorphism.Results: We identified that SNP miR-499 rs3746444 A>G increased the susceptibility of AEG (AG vs AA: adjusted OR=1.25, 95% CI=1.05–1.49, P=0.012 and AG/GG vs AA: adjusted OR=1.30, 95% CI=1.10–1.54, P=0.002). In a stratified analysis, we found that miR-499 rs3746444 A>G polymorphism had an increased susceptibility of AEG in several subgroups (male subgroup: AG vs AA: adjusted P=0.004 and AG/GG vs AA: adjusted P=0.002; female subgroup: GG vs AG/AA: adjusted P=0.046; <64 years subgroup: AG vs AA: adjusted P=0.006 and AG/GG vs AA: adjusted P=0.003; never smoking subgroup: AG vs AA: adjusted P=0.003 and AG/GG vs AA: adjusted P=0.001; and never drinking subgroup: AG vs AA: adjusted P=0.008 and AG/GG vs AA: adjusted P=0.002). The results of power calculation indicated that miR-499 rs3746444 A>G polymorphism increased the risk of AEG in overall comparison, male, <64 years, never smoking, and never drinking subgroups. Among the AEG cases, 625 patients accompanied by positive lymph node. However, the distribution of miRNA-499 rs3746444 A>G variants was no significant difference between different lymph node status.Conclusion: Our findings indicate that miR-499 rs3746444 A>G polymorphism is significantly associated with AEG susceptibility. In the future, further exploration of this genetic factor in relation to AEG susceptibility with an adequate methodological quality is needed.Keywords: miRNA-499, polymorphism, susceptibility, lymph node metastasis, adenocarcinoma of esophagogastric junctionhttps://www.dovepress.com/association-of-mirna-499-rs3746444-agtg-variants-with-adenocarcinoma-o-peer-reviewed-article-OTTmiRNA-499polymorphismsusceptibilitylymph node metastasisadenocarcinoma of esophagogastric junction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tang W Wang Y Pan H Qiu H Chen S |
spellingShingle |
Tang W Wang Y Pan H Qiu H Chen S Association of miRNA-499 rs3746444 A>G variants with adenocarcinoma of esophagogastric junction (AEG) risk and lymph node status OncoTargets and Therapy miRNA-499 polymorphism susceptibility lymph node metastasis adenocarcinoma of esophagogastric junction |
author_facet |
Tang W Wang Y Pan H Qiu H Chen S |
author_sort |
Tang W |
title |
Association of miRNA-499 rs3746444 A>G variants with adenocarcinoma of esophagogastric junction (AEG) risk and lymph node status |
title_short |
Association of miRNA-499 rs3746444 A>G variants with adenocarcinoma of esophagogastric junction (AEG) risk and lymph node status |
title_full |
Association of miRNA-499 rs3746444 A>G variants with adenocarcinoma of esophagogastric junction (AEG) risk and lymph node status |
title_fullStr |
Association of miRNA-499 rs3746444 A>G variants with adenocarcinoma of esophagogastric junction (AEG) risk and lymph node status |
title_full_unstemmed |
Association of miRNA-499 rs3746444 A>G variants with adenocarcinoma of esophagogastric junction (AEG) risk and lymph node status |
title_sort |
association of mirna-499 rs3746444 a>g variants with adenocarcinoma of esophagogastric junction (aeg) risk and lymph node status |
publisher |
Dove Medical Press |
series |
OncoTargets and Therapy |
issn |
1178-6930 |
publishDate |
2019-08-01 |
description |
Weifeng Tang,1,* Yafeng Wang,2,* Huiwen Pan,1 Hao Qiu,3 Shuchen Chen41Department of Cardiothoracic Surgery, Affiliated People’s Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, People’s Republic of China; 2Department of Cardiology, The People’s Hospital of Xishuangbanna dai Autonomous Prefecture, Jinghong, Yunnan Province, People’s Republic of China; 3Department of Immunology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu Province, People’s Republic of China; 4Department of Thoracic Surgery, Fujian Medical University Union Hospital, Fuzhou, Fujian Province, People’s Republic of China*These authors contributed equally to this workBackground: MicroRNAs (miRNAs) miRNA-499 rs3746444 A>G polymorphism may be complicated in the susceptibility to cancer. However, the correlation of this polymorphism with adenocarcinoma of esophagogastric junction (AEG) was unknown.Patients and methods: A total of 1063 AEG patients and 1677 controls were included in this study to assess the association of miR-499 rs3746444 A>G with AEG risk. SNPscanTM genotyping assay was harnessed to obtain the genotypes of miRNA-499 rs3746444 A>G polymorphism.Results: We identified that SNP miR-499 rs3746444 A>G increased the susceptibility of AEG (AG vs AA: adjusted OR=1.25, 95% CI=1.05–1.49, P=0.012 and AG/GG vs AA: adjusted OR=1.30, 95% CI=1.10–1.54, P=0.002). In a stratified analysis, we found that miR-499 rs3746444 A>G polymorphism had an increased susceptibility of AEG in several subgroups (male subgroup: AG vs AA: adjusted P=0.004 and AG/GG vs AA: adjusted P=0.002; female subgroup: GG vs AG/AA: adjusted P=0.046; <64 years subgroup: AG vs AA: adjusted P=0.006 and AG/GG vs AA: adjusted P=0.003; never smoking subgroup: AG vs AA: adjusted P=0.003 and AG/GG vs AA: adjusted P=0.001; and never drinking subgroup: AG vs AA: adjusted P=0.008 and AG/GG vs AA: adjusted P=0.002). The results of power calculation indicated that miR-499 rs3746444 A>G polymorphism increased the risk of AEG in overall comparison, male, <64 years, never smoking, and never drinking subgroups. Among the AEG cases, 625 patients accompanied by positive lymph node. However, the distribution of miRNA-499 rs3746444 A>G variants was no significant difference between different lymph node status.Conclusion: Our findings indicate that miR-499 rs3746444 A>G polymorphism is significantly associated with AEG susceptibility. In the future, further exploration of this genetic factor in relation to AEG susceptibility with an adequate methodological quality is needed.Keywords: miRNA-499, polymorphism, susceptibility, lymph node metastasis, adenocarcinoma of esophagogastric junction |
topic |
miRNA-499 polymorphism susceptibility lymph node metastasis adenocarcinoma of esophagogastric junction |
url |
https://www.dovepress.com/association-of-mirna-499-rs3746444-agtg-variants-with-adenocarcinoma-o-peer-reviewed-article-OTT |
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