Overexpression of <i>SgGH3.1</i> from Fine-Stem Stylo (<i>Stylosanthes guianensis</i> var. <i>intermedia</i>) Enhances Chilling and Cold Tolerance in <i>Arabidopsis thaliana</i>
<i>Stylosanthes</i> (stylo) species are commercially significant tropical and subtropical forage and pasture legumes that are vulnerable to chilling and frost. However, little is known about the molecular mechanisms behind stylos’ responses to low temperature stress. Gretchen-Hagen 3 (GH...
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doaj-f8168fde6e8e4957b195a384bc01799e2021-09-26T00:13:13ZengMDPI AGGenes2073-44252021-08-01121367136710.3390/genes12091367Overexpression of <i>SgGH3.1</i> from Fine-Stem Stylo (<i>Stylosanthes guianensis</i> var. <i>intermedia</i>) Enhances Chilling and Cold Tolerance in <i>Arabidopsis thaliana</i>Ming Jiang0Long-Long Ma1Huai-An Huang2Shan-Wen Ke3Chun-Sheng Gui4Xin-Yi Ning5Xiang-Qian Zhang6Tian-Xiu Zhong7Xin-Ming Xie8Shu Chen9Department of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaDepartment of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaDepartment of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaGansu Engineering Laboratory of Applied Mycology, Hexi University, Zhangye 734000, ChinaDepartment of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaDepartment of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaDepartment of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaDepartment of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Engineering Research Center for Grassland Science, Guangzhou 510642, ChinaDepartment of Grassland Science, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China<i>Stylosanthes</i> (stylo) species are commercially significant tropical and subtropical forage and pasture legumes that are vulnerable to chilling and frost. However, little is known about the molecular mechanisms behind stylos’ responses to low temperature stress. Gretchen-Hagen 3 (GH3) proteins have been extensively investigated in many plant species for their roles in auxin homeostasis and abiotic stress responses, but none have been reported in stylos. <i>SgGH3.1</i>, a cold-responsive gene identified in a whole transcriptome profiling study of fine-stem stylo (<i>S. guianensis</i> var. <i>intermedia</i>) was further investigated for its involvement in cold stress tolerance. <i>SgGH3.1</i> shared a high percentage of identity with 14 leguminous GH3 proteins, ranging from 79% to 93%. Phylogenetic analysis classified SgGH3.1 into Group Ⅱ of GH3 family, which have been proven to involve with auxins conjugation. Expression profiling revealed that <i>SgGH3.1</i> responded rapidly to cold stress in stylo leaves. Overexpression of <i>SgGH3.1</i> in <i>Arabidopsis thaliana</i> altered sensitivity to exogenous IAA, up-regulated transcription of <i>AtCBF1-3</i> genes, activated physiological responses against cold stress, and enhanced chilling and cold tolerances. This is the first report of a <i>GH3</i> gene in stylos, which not only validated its function in IAA homeostasis and cold responses, but also gave insight into breeding of cold-tolerant stylos.https://www.mdpi.com/2073-4425/12/9/1367Gretchen-Hagen 3<i>Stylosanthes</i>overexpressiontransgeniccold toleranceauxin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ming Jiang Long-Long Ma Huai-An Huang Shan-Wen Ke Chun-Sheng Gui Xin-Yi Ning Xiang-Qian Zhang Tian-Xiu Zhong Xin-Ming Xie Shu Chen |
spellingShingle |
Ming Jiang Long-Long Ma Huai-An Huang Shan-Wen Ke Chun-Sheng Gui Xin-Yi Ning Xiang-Qian Zhang Tian-Xiu Zhong Xin-Ming Xie Shu Chen Overexpression of <i>SgGH3.1</i> from Fine-Stem Stylo (<i>Stylosanthes guianensis</i> var. <i>intermedia</i>) Enhances Chilling and Cold Tolerance in <i>Arabidopsis thaliana</i> Genes Gretchen-Hagen 3 <i>Stylosanthes</i> overexpression transgenic cold tolerance auxin |
author_facet |
Ming Jiang Long-Long Ma Huai-An Huang Shan-Wen Ke Chun-Sheng Gui Xin-Yi Ning Xiang-Qian Zhang Tian-Xiu Zhong Xin-Ming Xie Shu Chen |
author_sort |
Ming Jiang |
title |
Overexpression of <i>SgGH3.1</i> from Fine-Stem Stylo (<i>Stylosanthes guianensis</i> var. <i>intermedia</i>) Enhances Chilling and Cold Tolerance in <i>Arabidopsis thaliana</i> |
title_short |
Overexpression of <i>SgGH3.1</i> from Fine-Stem Stylo (<i>Stylosanthes guianensis</i> var. <i>intermedia</i>) Enhances Chilling and Cold Tolerance in <i>Arabidopsis thaliana</i> |
title_full |
Overexpression of <i>SgGH3.1</i> from Fine-Stem Stylo (<i>Stylosanthes guianensis</i> var. <i>intermedia</i>) Enhances Chilling and Cold Tolerance in <i>Arabidopsis thaliana</i> |
title_fullStr |
Overexpression of <i>SgGH3.1</i> from Fine-Stem Stylo (<i>Stylosanthes guianensis</i> var. <i>intermedia</i>) Enhances Chilling and Cold Tolerance in <i>Arabidopsis thaliana</i> |
title_full_unstemmed |
Overexpression of <i>SgGH3.1</i> from Fine-Stem Stylo (<i>Stylosanthes guianensis</i> var. <i>intermedia</i>) Enhances Chilling and Cold Tolerance in <i>Arabidopsis thaliana</i> |
title_sort |
overexpression of <i>sggh3.1</i> from fine-stem stylo (<i>stylosanthes guianensis</i> var. <i>intermedia</i>) enhances chilling and cold tolerance in <i>arabidopsis thaliana</i> |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2021-08-01 |
description |
<i>Stylosanthes</i> (stylo) species are commercially significant tropical and subtropical forage and pasture legumes that are vulnerable to chilling and frost. However, little is known about the molecular mechanisms behind stylos’ responses to low temperature stress. Gretchen-Hagen 3 (GH3) proteins have been extensively investigated in many plant species for their roles in auxin homeostasis and abiotic stress responses, but none have been reported in stylos. <i>SgGH3.1</i>, a cold-responsive gene identified in a whole transcriptome profiling study of fine-stem stylo (<i>S. guianensis</i> var. <i>intermedia</i>) was further investigated for its involvement in cold stress tolerance. <i>SgGH3.1</i> shared a high percentage of identity with 14 leguminous GH3 proteins, ranging from 79% to 93%. Phylogenetic analysis classified SgGH3.1 into Group Ⅱ of GH3 family, which have been proven to involve with auxins conjugation. Expression profiling revealed that <i>SgGH3.1</i> responded rapidly to cold stress in stylo leaves. Overexpression of <i>SgGH3.1</i> in <i>Arabidopsis thaliana</i> altered sensitivity to exogenous IAA, up-regulated transcription of <i>AtCBF1-3</i> genes, activated physiological responses against cold stress, and enhanced chilling and cold tolerances. This is the first report of a <i>GH3</i> gene in stylos, which not only validated its function in IAA homeostasis and cold responses, but also gave insight into breeding of cold-tolerant stylos. |
topic |
Gretchen-Hagen 3 <i>Stylosanthes</i> overexpression transgenic cold tolerance auxin |
url |
https://www.mdpi.com/2073-4425/12/9/1367 |
work_keys_str_mv |
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