Identification and Expression Analysis of Glutamine Synthetase Genes in Ramie (Boehmeria nivea L. Gaud)
Glutamine synthetase (GS) plays a fundamental role in nitrogen metabolism in higher plants. Three BnGS genes have first been isolated: one gene encoding plastid GS (BnGS2) and two encoding cytosolic GS (BnGS1-1 and BnGS1-2) in ramie. Based on a sequence analysis and phylogenetic study, three BnGS se...
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doaj-f817f5adb8aa4956ad86ab28a4d7befc2020-11-24T22:07:57ZengDe GruyterOpen Life Sciences2391-54122015-06-0110110.1515/biol-2015-0032biol-2015-0032Identification and Expression Analysis of Glutamine Synthetase Genes in Ramie (Boehmeria nivea L. Gaud)Zheng Jian-Shu0Yu Chun-Ming1Chen Ping2Wang Yan-Zhou3Liu Tou-Ming4Xiong He-Ping5Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, PR ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, PR ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, PR ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, PR ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, PR ChinaInstitute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, PR ChinaGlutamine synthetase (GS) plays a fundamental role in nitrogen metabolism in higher plants. Three BnGS genes have first been isolated: one gene encoding plastid GS (BnGS2) and two encoding cytosolic GS (BnGS1-1 and BnGS1-2) in ramie. Based on a sequence analysis and phylogenetic study, three BnGS sequences were classified into three distinct sub-families. The phylogenetic analysis showed that BnGS2 and BnGS1-2 were closely related to those of legumes, alfalfa (Medicago sativa), soybean (Glycine max) and bean (Phaseolus vulgaris). The BnGS gene expression patterns revealed that each gene exhibited similar organ specificity, but distinct transcript intensity during different vegetative processes. The relatively abundant expression of BnGS1-1 and BnGS2 at specific organs during different vegetative processes indicates that they have critical roles in nitrogen uptake and assimilation relating to forage and growth characteristics. The BnGS1-2 mRNA levels were remarkably upregulated in the phloem, xylem and stems during the fiber development stage, suggesting a correlation with fiber development. Therefore, the non-overlapping transcript intensity of BnGS genes in different tissues regulates ramie growth and development during different vegetative processes.http://www.degruyter.com/view/j/biol.2015.10.issue-1/biol-2015-0032/biol-2015-0032.xml?format=INTBoehmeria nivea L. Gaud Glutamine synthetaseVegetative processExpression patterns Forage characteristics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zheng Jian-Shu Yu Chun-Ming Chen Ping Wang Yan-Zhou Liu Tou-Ming Xiong He-Ping |
spellingShingle |
Zheng Jian-Shu Yu Chun-Ming Chen Ping Wang Yan-Zhou Liu Tou-Ming Xiong He-Ping Identification and Expression Analysis of Glutamine Synthetase Genes in Ramie (Boehmeria nivea L. Gaud) Open Life Sciences Boehmeria nivea L. Gaud Glutamine synthetase Vegetative process Expression patterns Forage characteristics |
author_facet |
Zheng Jian-Shu Yu Chun-Ming Chen Ping Wang Yan-Zhou Liu Tou-Ming Xiong He-Ping |
author_sort |
Zheng Jian-Shu |
title |
Identification and Expression Analysis of
Glutamine Synthetase Genes in Ramie (Boehmeria nivea L. Gaud) |
title_short |
Identification and Expression Analysis of
Glutamine Synthetase Genes in Ramie (Boehmeria nivea L. Gaud) |
title_full |
Identification and Expression Analysis of
Glutamine Synthetase Genes in Ramie (Boehmeria nivea L. Gaud) |
title_fullStr |
Identification and Expression Analysis of
Glutamine Synthetase Genes in Ramie (Boehmeria nivea L. Gaud) |
title_full_unstemmed |
Identification and Expression Analysis of
Glutamine Synthetase Genes in Ramie (Boehmeria nivea L. Gaud) |
title_sort |
identification and expression analysis of
glutamine synthetase genes in ramie (boehmeria nivea l. gaud) |
publisher |
De Gruyter |
series |
Open Life Sciences |
issn |
2391-5412 |
publishDate |
2015-06-01 |
description |
Glutamine synthetase (GS) plays a fundamental
role in nitrogen metabolism in higher plants. Three BnGS
genes have first been isolated: one gene encoding plastid
GS (BnGS2) and two encoding cytosolic GS (BnGS1-1 and
BnGS1-2) in ramie. Based on a sequence analysis and
phylogenetic study, three BnGS sequences were classified
into three distinct sub-families. The phylogenetic analysis
showed that BnGS2 and BnGS1-2 were closely related
to those of legumes, alfalfa (Medicago sativa), soybean
(Glycine max) and bean (Phaseolus vulgaris). The BnGS
gene expression patterns revealed that each gene exhibited
similar organ specificity, but distinct transcript intensity
during different vegetative processes. The relatively
abundant expression of BnGS1-1 and BnGS2 at specific
organs during different vegetative processes indicates that
they have critical roles in nitrogen uptake and assimilation
relating to forage and growth characteristics. The BnGS1-2
mRNA levels were remarkably upregulated in the
phloem, xylem and stems during the fiber development
stage, suggesting a correlation with fiber development.
Therefore, the non-overlapping transcript intensity of
BnGS genes in different tissues regulates ramie growth
and development during different vegetative processes. |
topic |
Boehmeria nivea L. Gaud Glutamine synthetase Vegetative process Expression patterns Forage characteristics |
url |
http://www.degruyter.com/view/j/biol.2015.10.issue-1/biol-2015-0032/biol-2015-0032.xml?format=INT |
work_keys_str_mv |
AT zhengjianshu identificationandexpressionanalysisofglutaminesynthetasegenesinramieboehmerianivealgaud AT yuchunming identificationandexpressionanalysisofglutaminesynthetasegenesinramieboehmerianivealgaud AT chenping identificationandexpressionanalysisofglutaminesynthetasegenesinramieboehmerianivealgaud AT wangyanzhou identificationandexpressionanalysisofglutaminesynthetasegenesinramieboehmerianivealgaud AT liutouming identificationandexpressionanalysisofglutaminesynthetasegenesinramieboehmerianivealgaud AT xiongheping identificationandexpressionanalysisofglutaminesynthetasegenesinramieboehmerianivealgaud |
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