Short-term differential adaptation to anaerobic stress via genomic mutations by Escherichia coli strains K-12 and B lacking alcohol dehydrogenase
Microbial adaptations often occur via genomic mutations under adverse environmental conditions. This study used Escherichia coli adhE cells as a model system to investigate adaptation to anaerobic conditions, which we then compared with the adaptive mechanisms of two closely related E. coli strains...
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doaj-f83b301bf7ed486d88bd6e8c5e6d26ba2020-11-24T23:54:01ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2014-09-01510.3389/fmicb.2014.00476102720Short-term differential adaptation to anaerobic stress via genomic mutations by Escherichia coli strains K-12 and B lacking alcohol dehydrogenaseHyun Ju eKim0Hyun Ju eKim1Haeyoung eJeong2Haeyoung eJeong3Seungwoo eHwang4Moo-Seung eLee5Yong-Jik eLee6Dong-Woo eLee7Sang Jun eLee8Sang Jun eLee9Korea Research Insitutute of Bioscience and BiotechnologyKorea University of Science and TechnologyKorea Research Insitutute of Bioscience and BiotechnologyKorea University of Science and TechnologyKorea Research Insitutute of Bioscience and BiotechnologyKorea Research Insitutute of Bioscience and BiotechnologyKyungpook National UniversityKyungpook National UniversityKorea Research Insitutute of Bioscience and BiotechnologyKorea University of Science and TechnologyMicrobial adaptations often occur via genomic mutations under adverse environmental conditions. This study used Escherichia coli adhE cells as a model system to investigate adaptation to anaerobic conditions, which we then compared with the adaptive mechanisms of two closely related E. coli strains, K-12 and B. In contrast to K-12 adhE cells, the E. coli B adhE cells exhibited significantly delayed adaptive growth under anaerobic conditions. Adaptation by the K-12 and B strains mainly employed anaerobic lactate fermentation to restore cellular growth. Several mutations were identified in the pta or pflB genes of adapted K-12 cells, but mostly in the pta gene of the B strains. However, the types of mutation in the adapted K-12 and B strains were similar. Cellular viability was affected directly by severe redox imbalance in B adhE cells, which also impaired their ability to adapt to anaerobic conditions.This study demonstrates that closely related microorganisms may undergo different adaptations under the same set of adverse conditions, which might be associated with the specific metabolic characteristics of each strain. This study provides new insights into short-term microbial adaptation to stressful conditions, which may reflect dynamic microbial population changes in nature.http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00476/fullAlcohol Dehydrogenaseadaptationanaerobic conditiongenomic mutationredox balancepta gene |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hyun Ju eKim Hyun Ju eKim Haeyoung eJeong Haeyoung eJeong Seungwoo eHwang Moo-Seung eLee Yong-Jik eLee Dong-Woo eLee Sang Jun eLee Sang Jun eLee |
spellingShingle |
Hyun Ju eKim Hyun Ju eKim Haeyoung eJeong Haeyoung eJeong Seungwoo eHwang Moo-Seung eLee Yong-Jik eLee Dong-Woo eLee Sang Jun eLee Sang Jun eLee Short-term differential adaptation to anaerobic stress via genomic mutations by Escherichia coli strains K-12 and B lacking alcohol dehydrogenase Frontiers in Microbiology Alcohol Dehydrogenase adaptation anaerobic condition genomic mutation redox balance pta gene |
author_facet |
Hyun Ju eKim Hyun Ju eKim Haeyoung eJeong Haeyoung eJeong Seungwoo eHwang Moo-Seung eLee Yong-Jik eLee Dong-Woo eLee Sang Jun eLee Sang Jun eLee |
author_sort |
Hyun Ju eKim |
title |
Short-term differential adaptation to anaerobic stress via genomic mutations by Escherichia coli strains K-12 and B lacking alcohol dehydrogenase |
title_short |
Short-term differential adaptation to anaerobic stress via genomic mutations by Escherichia coli strains K-12 and B lacking alcohol dehydrogenase |
title_full |
Short-term differential adaptation to anaerobic stress via genomic mutations by Escherichia coli strains K-12 and B lacking alcohol dehydrogenase |
title_fullStr |
Short-term differential adaptation to anaerobic stress via genomic mutations by Escherichia coli strains K-12 and B lacking alcohol dehydrogenase |
title_full_unstemmed |
Short-term differential adaptation to anaerobic stress via genomic mutations by Escherichia coli strains K-12 and B lacking alcohol dehydrogenase |
title_sort |
short-term differential adaptation to anaerobic stress via genomic mutations by escherichia coli strains k-12 and b lacking alcohol dehydrogenase |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2014-09-01 |
description |
Microbial adaptations often occur via genomic mutations under adverse environmental conditions. This study used Escherichia coli adhE cells as a model system to investigate adaptation to anaerobic conditions, which we then compared with the adaptive mechanisms of two closely related E. coli strains, K-12 and B. In contrast to K-12 adhE cells, the E. coli B adhE cells exhibited significantly delayed adaptive growth under anaerobic conditions. Adaptation by the K-12 and B strains mainly employed anaerobic lactate fermentation to restore cellular growth. Several mutations were identified in the pta or pflB genes of adapted K-12 cells, but mostly in the pta gene of the B strains. However, the types of mutation in the adapted K-12 and B strains were similar. Cellular viability was affected directly by severe redox imbalance in B adhE cells, which also impaired their ability to adapt to anaerobic conditions.This study demonstrates that closely related microorganisms may undergo different adaptations under the same set of adverse conditions, which might be associated with the specific metabolic characteristics of each strain. This study provides new insights into short-term microbial adaptation to stressful conditions, which may reflect dynamic microbial population changes in nature. |
topic |
Alcohol Dehydrogenase adaptation anaerobic condition genomic mutation redox balance pta gene |
url |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00476/full |
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