Alternatives for large-scale production of cultured beef: A review
Cultured beef is a method where stem cells from skeletal muscle of cows are cultured in vitro to gain edible muscle tissue. For large-scale production of cultured beef, the culture technique needs to become more efficient than today's 2-dimensional (2D) standard technique that was used to make...
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doaj-f8453326a9c24d29802af930a166f3fe2021-06-07T06:50:32ZengElsevierJournal of Integrative Agriculture2095-31192015-02-01142208216Alternatives for large-scale production of cultured beef: A reviewMatilda S M Moritz0Sanne E L Verbruggen1Mark J Post2Department of Physiology, Maastricht University, Maastricht 6229 ER, The Netherlands; Department of Physics, Chemistry and Biology, Linköping University, Linköping SE-581 83, SwedenDepartment of Physiology, Maastricht University, Maastricht 6229 ER, The NetherlandsDepartment of Physiology, Maastricht University, Maastricht 6229 ER, The Netherlands; Correspondence Mark J Post, Tel: +31-43-3881200, Mobile: +31-6-46705558, Fax: +31-43-3884166Cultured beef is a method where stem cells from skeletal muscle of cows are cultured in vitro to gain edible muscle tissue. For large-scale production of cultured beef, the culture technique needs to become more efficient than today's 2-dimensional (2D) standard technique that was used to make the first cultured hamburger. Options for efficient large-scale production of stem cells are to culture cells on microcarriers, either in suspension or in a packed bed bioreactor, or to culture aggregated cells in suspension. We discuss the pros and cons of these systems as well as the possibilities to use the systems for tissue culture. Either of the production systems needs to be optimized to achieve an efficient production of cultured beef. It is anticipated that the optimization of large-scale cell culture as performed for other stem cells can be translated into successful protocols for bovine satellite cells resulting in resource and cost efficient cultured beef.http://www.sciencedirect.com/science/article/pii/S2095311914608893cultured beefmicrocarriersaggregated cellspacked bed bioreactorcell culture |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Matilda S M Moritz Sanne E L Verbruggen Mark J Post |
spellingShingle |
Matilda S M Moritz Sanne E L Verbruggen Mark J Post Alternatives for large-scale production of cultured beef: A review Journal of Integrative Agriculture cultured beef microcarriers aggregated cells packed bed bioreactor cell culture |
author_facet |
Matilda S M Moritz Sanne E L Verbruggen Mark J Post |
author_sort |
Matilda S M Moritz |
title |
Alternatives for large-scale production of cultured beef: A review |
title_short |
Alternatives for large-scale production of cultured beef: A review |
title_full |
Alternatives for large-scale production of cultured beef: A review |
title_fullStr |
Alternatives for large-scale production of cultured beef: A review |
title_full_unstemmed |
Alternatives for large-scale production of cultured beef: A review |
title_sort |
alternatives for large-scale production of cultured beef: a review |
publisher |
Elsevier |
series |
Journal of Integrative Agriculture |
issn |
2095-3119 |
publishDate |
2015-02-01 |
description |
Cultured beef is a method where stem cells from skeletal muscle of cows are cultured in vitro to gain edible muscle tissue. For large-scale production of cultured beef, the culture technique needs to become more efficient than today's 2-dimensional (2D) standard technique that was used to make the first cultured hamburger. Options for efficient large-scale production of stem cells are to culture cells on microcarriers, either in suspension or in a packed bed bioreactor, or to culture aggregated cells in suspension. We discuss the pros and cons of these systems as well as the possibilities to use the systems for tissue culture. Either of the production systems needs to be optimized to achieve an efficient production of cultured beef. It is anticipated that the optimization of large-scale cell culture as performed for other stem cells can be translated into successful protocols for bovine satellite cells resulting in resource and cost efficient cultured beef. |
topic |
cultured beef microcarriers aggregated cells packed bed bioreactor cell culture |
url |
http://www.sciencedirect.com/science/article/pii/S2095311914608893 |
work_keys_str_mv |
AT matildasmmoritz alternativesforlargescaleproductionofculturedbeefareview AT sanneelverbruggen alternativesforlargescaleproductionofculturedbeefareview AT markjpost alternativesforlargescaleproductionofculturedbeefareview |
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