Immunohistochemistry for Pathologists: Protocols, Pitfalls, and Tips
Immunohistochemistry (IHC) is an important auxiliary method for pathologists in routine diagnostic work as well as in basic and clinical research including exploration of biomarkers, as IHC allows confirmation of target molecule expressions in the context of microenvironment. Although there has been...
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Korean Society of Pathologists & the Korean Society for Cytopathology
2016-11-01
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Online Access: | http://www.jpatholtm.org/upload/pdf/jptm-2016-08-08.pdf |
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doaj-f84d526c82244d4882077b877ffa604a2020-11-24T21:27:58ZengKorean Society of Pathologists & the Korean Society for CytopathologyJournal of Pathology and Translational Medicine2383-78372383-78452016-11-0150641141810.4132/jptm.2016.08.0816651Immunohistochemistry for Pathologists: Protocols, Pitfalls, and TipsSo-Woon KimJin RohChan-Sik ParkImmunohistochemistry (IHC) is an important auxiliary method for pathologists in routine diagnostic work as well as in basic and clinical research including exploration of biomarkers, as IHC allows confirmation of target molecule expressions in the context of microenvironment. Although there has been a considerable progress in automation and standardization of IHC, there are still many things to be considered in proper optimization and appropriate interpretation. In this review, we aim to provide possible pitfalls and useful tips for practicing pathologists and residents in pathology training. First, general procedure of IHC is summarized, followed by pitfalls and tips in each step and a summary of troubleshooting. Second, ways to an accurate interpretation of IHC are discussed, with introduction to general quantification and analysis methods. This review is not intended to provide complete information on IHC, but to be used as a basic reference for practice and publication.http://www.jpatholtm.org/upload/pdf/jptm-2016-08-08.pdfImmmunohistochemistryAntigen-antibody reactionsImmunostainAuxiliary test |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
So-Woon Kim Jin Roh Chan-Sik Park |
spellingShingle |
So-Woon Kim Jin Roh Chan-Sik Park Immunohistochemistry for Pathologists: Protocols, Pitfalls, and Tips Journal of Pathology and Translational Medicine Immmunohistochemistry Antigen-antibody reactions Immunostain Auxiliary test |
author_facet |
So-Woon Kim Jin Roh Chan-Sik Park |
author_sort |
So-Woon Kim |
title |
Immunohistochemistry for Pathologists: Protocols, Pitfalls, and Tips |
title_short |
Immunohistochemistry for Pathologists: Protocols, Pitfalls, and Tips |
title_full |
Immunohistochemistry for Pathologists: Protocols, Pitfalls, and Tips |
title_fullStr |
Immunohistochemistry for Pathologists: Protocols, Pitfalls, and Tips |
title_full_unstemmed |
Immunohistochemistry for Pathologists: Protocols, Pitfalls, and Tips |
title_sort |
immunohistochemistry for pathologists: protocols, pitfalls, and tips |
publisher |
Korean Society of Pathologists & the Korean Society for Cytopathology |
series |
Journal of Pathology and Translational Medicine |
issn |
2383-7837 2383-7845 |
publishDate |
2016-11-01 |
description |
Immunohistochemistry (IHC) is an important auxiliary method for pathologists in routine diagnostic work as well as in basic and clinical research including exploration of biomarkers, as IHC allows confirmation of target molecule expressions in the context of microenvironment. Although there has been a considerable progress in automation and standardization of IHC, there are still many things to be considered in proper optimization and appropriate interpretation. In this review, we aim to provide possible pitfalls and useful tips for practicing pathologists and residents in pathology training. First, general procedure of IHC is summarized, followed by pitfalls and tips in each step and a summary of troubleshooting. Second, ways to an accurate interpretation of IHC are discussed, with introduction to general quantification and analysis methods. This review is not intended to provide complete information on IHC, but to be used as a basic reference for practice and publication. |
topic |
Immmunohistochemistry Antigen-antibody reactions Immunostain Auxiliary test |
url |
http://www.jpatholtm.org/upload/pdf/jptm-2016-08-08.pdf |
work_keys_str_mv |
AT sowoonkim immunohistochemistryforpathologistsprotocolspitfallsandtips AT jinroh immunohistochemistryforpathologistsprotocolspitfallsandtips AT chansikpark immunohistochemistryforpathologistsprotocolspitfallsandtips |
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1725972395757404160 |