MiR200 and miR302: Two Big Families Influencing Stem Cell Behavior
In this review, we described different factors that modulate pluripotency in stem cells, in particular we aimed at following the steps of two large families of miRNAs: the miR-200 family and the miR-302 family. We analyzed some factors tuning stem cells behavior as TGF-β, which plays a pivotal role...
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doaj-f8509630c0cd4520908a2b11ae39b0cf2020-11-24T22:27:37ZengMDPI AGMolecules1420-30492018-01-0123228210.3390/molecules23020282molecules23020282MiR200 and miR302: Two Big Families Influencing Stem Cell BehaviorFrancesca Balzano0Sara Cruciani1Valentina Basoli2Sara Santaniello3Federica Facchin4Carlo Ventura5Margherita Maioli6Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, Via Massarenti 9, 40138 Bologna, ItalyLaboratory of Molecular Biology and Stem Cell Engineering, National Institute of Biostructures and Biosystems, Innovation Accelerator, CNR, Via Piero Gobetti 101, 40129 Bologna, ItalyDepartment of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, ItalyIn this review, we described different factors that modulate pluripotency in stem cells, in particular we aimed at following the steps of two large families of miRNAs: the miR-200 family and the miR-302 family. We analyzed some factors tuning stem cells behavior as TGF-β, which plays a pivotal role in pluripotency inhibition together with specific miRNAs, reactive oxygen species (ROS), but also hypoxia, and physical stimuli, such as ad hoc conveyed electromagnetic fields. TGF-β plays a crucial role in the suppression of pluripotency thus influencing the achievement of a specific phenotype. ROS concentration can modulate TGF-β activation that in turns down regulates miR-200 and miR-302. These two miRNAs are usually requested to maintain pluripotency, while they are down-regulated during the acquirement of a specific cellular phenotype. Moreover, also physical stimuli, such as extremely-low frequency electromagnetic fields or high-frequency electromagnetic fields conveyed with a radioelectric asymmetric conveyer (REAC), and hypoxia can deeply influence stem cell behavior by inducing the appearance of specific phenotypes, as well as a direct reprogramming of somatic cells. Unraveling the molecular mechanisms underlying the complex interplay between externally applied stimuli and epigenetic events could disclose novel target molecules to commit stem cell fate.http://www.mdpi.com/1420-3049/23/2/282stem cellsmiRNAepigeneticscell reprogramming |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Francesca Balzano Sara Cruciani Valentina Basoli Sara Santaniello Federica Facchin Carlo Ventura Margherita Maioli |
spellingShingle |
Francesca Balzano Sara Cruciani Valentina Basoli Sara Santaniello Federica Facchin Carlo Ventura Margherita Maioli MiR200 and miR302: Two Big Families Influencing Stem Cell Behavior Molecules stem cells miRNA epigenetics cell reprogramming |
author_facet |
Francesca Balzano Sara Cruciani Valentina Basoli Sara Santaniello Federica Facchin Carlo Ventura Margherita Maioli |
author_sort |
Francesca Balzano |
title |
MiR200 and miR302: Two Big Families Influencing Stem Cell Behavior |
title_short |
MiR200 and miR302: Two Big Families Influencing Stem Cell Behavior |
title_full |
MiR200 and miR302: Two Big Families Influencing Stem Cell Behavior |
title_fullStr |
MiR200 and miR302: Two Big Families Influencing Stem Cell Behavior |
title_full_unstemmed |
MiR200 and miR302: Two Big Families Influencing Stem Cell Behavior |
title_sort |
mir200 and mir302: two big families influencing stem cell behavior |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2018-01-01 |
description |
In this review, we described different factors that modulate pluripotency in stem cells, in particular we aimed at following the steps of two large families of miRNAs: the miR-200 family and the miR-302 family. We analyzed some factors tuning stem cells behavior as TGF-β, which plays a pivotal role in pluripotency inhibition together with specific miRNAs, reactive oxygen species (ROS), but also hypoxia, and physical stimuli, such as ad hoc conveyed electromagnetic fields. TGF-β plays a crucial role in the suppression of pluripotency thus influencing the achievement of a specific phenotype. ROS concentration can modulate TGF-β activation that in turns down regulates miR-200 and miR-302. These two miRNAs are usually requested to maintain pluripotency, while they are down-regulated during the acquirement of a specific cellular phenotype. Moreover, also physical stimuli, such as extremely-low frequency electromagnetic fields or high-frequency electromagnetic fields conveyed with a radioelectric asymmetric conveyer (REAC), and hypoxia can deeply influence stem cell behavior by inducing the appearance of specific phenotypes, as well as a direct reprogramming of somatic cells. Unraveling the molecular mechanisms underlying the complex interplay between externally applied stimuli and epigenetic events could disclose novel target molecules to commit stem cell fate. |
topic |
stem cells miRNA epigenetics cell reprogramming |
url |
http://www.mdpi.com/1420-3049/23/2/282 |
work_keys_str_mv |
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