Adsorption to soils and biochemical characterization of commercial phytases
<p>Commercial phytases are widely used in poultry production, but little is known about their potential use as biofertilizer for agricultural crops as an alternative to reduce the use of synthetic fertilizers. Four purified phytases isolated from <i>Aspergillus niger</i> and <i&...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2020-04-01
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| Series: | SOIL |
| Online Access: | https://www.soil-journal.net/6/153/2020/soil-6-153-2020.pdf |
| Summary: | <p>Commercial phytases are widely used in poultry production, but
little is known about their potential use as biofertilizer for agricultural
crops as an alternative to reduce the use of synthetic fertilizers. Four
purified phytases isolated from <i>Aspergillus niger</i> and <i>Escherichia coli</i> were characterized biochemically and
in terms of their adsorption to soils belonging to the Mollisol order. Three
different organic P substrates were used to measure enzyme activity under a
wide range of pH (2.3 to 9) and temperatures (<span class="inline-formula">−</span>10 to
70 <span class="inline-formula"><sup>∘</sup></span>C) conditions: phytic acid, <span class="inline-formula"><i>p</i></span>-nitrophenyl-phosphate (<span class="inline-formula"><i>p</i></span>NP) and
glyceraldehyde-3-phosphate (G3Phosphate). Phytases had a low affinity for the
solid phase: 23 %–34 % of the added amount was adsorbed after 1 h of
incubation. Phytases from <i>A. niger</i> showed a higher capacity to release P (13 % on
average) than phytases from <i>E. coli</i>. All phytases were active throughout the pH and temperature ranges related to optimum crop production. At pH values commonly
found in agricultural soils (5.5–7), <i>A. niger</i> phytases released P with the following substrate ranking with respect to effectiveness: <span class="inline-formula"><i>p</i></span>NP > phytic acid > G3Phosphate. <i>E. coli</i> phytases, in comparison, released P following with the following substrate ranking with respect to effectiveness: <span class="inline-formula"><i>p</i></span>NP <span class="inline-formula">∕</span> phytic
acid > G3Phosphate. The results obtained are promising in terms of
the use of phytases as a complement to P fertilization in agricultural
settings and encourage further studies under field conditions.</p> |
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| ISSN: | 2199-3971 2199-398X |