In Vitro Synthesized RNA Generated from cDNA Clones of Both Genomic Components of Cucurbit yellow stunting disorder virus Replicates in Cucumber Protoplasts

In Vitro Synthesized RNA Generated from cDNA Clones of Both Genomic Components of Cucurbit yellow stunting disorder virus Replicates in Cucumber Protoplasts

Cucurbit yellow stunting disorder virus (CYSDV), a bipartite whitefly-transmitted virus, constitutes a major threat to commercial cucurbit production worldwide. Here, construction of full-length CYSDV RNA1 and RNA2 cDNA clones allowed the in vitro synthesis of RNA transcripts able to replicate in cu...

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Bibliographic Details
Main Authors: Carolyn A. Owen, Romy Moukarzel, Xiao Huang, Mona A. Kassem, Eleonora Eliasco, Miguel A. Aranda, Robert H. A. Coutts, Ioannis C. Livieratos
Format: Article
Language:English
Published: MDPI AG 2016-06-01
Series:Viruses
Subjects:
criniviruses
whitefly-transmitted viruses
Cucurbit yellow stunting disorder virus
infectious clones
protoplasts
Online Access:http://www.mdpi.com/1999-4915/8/6/170

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