Flow cytometric characterization of tissue-resident lymphocytes after murine liver and heart transplantation

• Main Authors: Amy Prosser, Sarah Dart, Irma Larma-Cornwall, Michaela Lucas
• Format: Article
• Language: English
• Published: Elsevier 2021-12-01
• Series: STAR Protocols
• Subjects: Cell isolation; Flow Cytometry/Mass Cytometry; Immunology
• Online Access: http://www.sciencedirect.com/science/article/pii/S2666166721005165

Summary: Alterations to organ biology caused by transplantation can have major impacts on the outcome. Tissue-resident lymphocytes normally maintain an organ’s immunity and function and are transferred during transplantation. Here, we provide a detailed protocol for the isolation of leukocytes, including tissue-resident lymphocytes, from transplanted livers and hearts in mice. Phenotypic and functional analysis of conventional and unconventional T cells by flow cytometry is included. This protocol can also be used for the effective isolation of leukocytes from non-transplanted livers and hearts.For complete details on the use and execution of this protocol, please refer to Prosser et al. (2021).