Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon
Zika virus (ZIKV) is a global public health emergency due to its association with microcephaly, Guillain-Barré syndrome, neuropathy, and myelitis in children and adults. A total of 87 countries have had evidence of autochthonous mosquito-borne transmission of ZIKV, distributed across four continents...
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doaj-f890e3f0e67f4dc1a25d3a34e337ccd02021-02-25T09:23:25ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2021-02-011210.3389/fphys.2021.642237642237Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference PhenomenonMichele Martins0Luis Felipe Costa Ramos1Jimmy Rodriguez Murillo2André Torres3Stephanie Serafim de Carvalho4Gilberto Barbosa Domont5Danielle Maria Perpétua de Oliveira6Rafael Dias Mesquita7Fábio César Sousa Nogueira8Rafael Maciel-de-Freitas9Magno Junqueira10Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilDepartamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilDivision of Chemistry I, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, SwedenCarlos Chagas Filho Biophysics Institute, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilDepartamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilDepartamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilDepartamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilDepartamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilDepartamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Mosquitos Transmissores de Hematozoários, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, BrazilDepartamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilZika virus (ZIKV) is a global public health emergency due to its association with microcephaly, Guillain-Barré syndrome, neuropathy, and myelitis in children and adults. A total of 87 countries have had evidence of autochthonous mosquito-borne transmission of ZIKV, distributed across four continents, and no antivirus therapy or vaccines are available. Therefore, several strategies have been developed to target the main mosquito vector, Aedes aegypti, to reduce the burden of different arboviruses. Among such strategies, the use of the maternally-inherited endosymbiont Wolbachia pipientis has been applied successfully to reduce virus susceptibility and decrease transmission. However, the mechanisms by which Wolbachia orchestrate resistance to ZIKV infection remain to be elucidated. In this study, we apply isobaric labeling quantitative mass spectrometry (MS)-based proteomics to quantify proteins and identify pathways altered during ZIKV infection; Wolbachia infection; co-infection with Wolbachia/ZIKV in the A. aegypti heads and salivary glands. We show that Wolbachia regulates proteins involved in reactive oxygen species production, regulates humoral immune response, and antioxidant production. The reduction of ZIKV polyprotein in the presence of Wolbachia in mosquitoes was determined by MS and corroborates the idea that Wolbachia helps to block ZIKV infections in A. aegypti. The present study offers a rich resource of data that may help to elucidate mechanisms by which Wolbachia orchestrate resistance to ZIKV infection in A. aegypti, and represents a step further on the development of new targeted methods to detect and quantify ZIKV and Wolbachia directly in complex tissues.https://www.frontiersin.org/articles/10.3389/fphys.2021.642237/fullproteomequantitativeAedes aegyptiWolbachiaZika virusimmune response |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Michele Martins Luis Felipe Costa Ramos Jimmy Rodriguez Murillo André Torres Stephanie Serafim de Carvalho Gilberto Barbosa Domont Danielle Maria Perpétua de Oliveira Rafael Dias Mesquita Fábio César Sousa Nogueira Rafael Maciel-de-Freitas Magno Junqueira |
spellingShingle |
Michele Martins Luis Felipe Costa Ramos Jimmy Rodriguez Murillo André Torres Stephanie Serafim de Carvalho Gilberto Barbosa Domont Danielle Maria Perpétua de Oliveira Rafael Dias Mesquita Fábio César Sousa Nogueira Rafael Maciel-de-Freitas Magno Junqueira Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon Frontiers in Physiology proteome quantitative Aedes aegypti Wolbachia Zika virus immune response |
author_facet |
Michele Martins Luis Felipe Costa Ramos Jimmy Rodriguez Murillo André Torres Stephanie Serafim de Carvalho Gilberto Barbosa Domont Danielle Maria Perpétua de Oliveira Rafael Dias Mesquita Fábio César Sousa Nogueira Rafael Maciel-de-Freitas Magno Junqueira |
author_sort |
Michele Martins |
title |
Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon |
title_short |
Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon |
title_full |
Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon |
title_fullStr |
Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon |
title_full_unstemmed |
Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon |
title_sort |
comprehensive quantitative proteome analysis of aedes aegypti identifies proteins and pathways involved in wolbachia pipientis and zika virus interference phenomenon |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Physiology |
issn |
1664-042X |
publishDate |
2021-02-01 |
description |
Zika virus (ZIKV) is a global public health emergency due to its association with microcephaly, Guillain-Barré syndrome, neuropathy, and myelitis in children and adults. A total of 87 countries have had evidence of autochthonous mosquito-borne transmission of ZIKV, distributed across four continents, and no antivirus therapy or vaccines are available. Therefore, several strategies have been developed to target the main mosquito vector, Aedes aegypti, to reduce the burden of different arboviruses. Among such strategies, the use of the maternally-inherited endosymbiont Wolbachia pipientis has been applied successfully to reduce virus susceptibility and decrease transmission. However, the mechanisms by which Wolbachia orchestrate resistance to ZIKV infection remain to be elucidated. In this study, we apply isobaric labeling quantitative mass spectrometry (MS)-based proteomics to quantify proteins and identify pathways altered during ZIKV infection; Wolbachia infection; co-infection with Wolbachia/ZIKV in the A. aegypti heads and salivary glands. We show that Wolbachia regulates proteins involved in reactive oxygen species production, regulates humoral immune response, and antioxidant production. The reduction of ZIKV polyprotein in the presence of Wolbachia in mosquitoes was determined by MS and corroborates the idea that Wolbachia helps to block ZIKV infections in A. aegypti. The present study offers a rich resource of data that may help to elucidate mechanisms by which Wolbachia orchestrate resistance to ZIKV infection in A. aegypti, and represents a step further on the development of new targeted methods to detect and quantify ZIKV and Wolbachia directly in complex tissues. |
topic |
proteome quantitative Aedes aegypti Wolbachia Zika virus immune response |
url |
https://www.frontiersin.org/articles/10.3389/fphys.2021.642237/full |
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