A Genomic Study of Salmonella Typhi ‘Vi’ Specific Bacteriophages: Futuristic Approach in Therapeutics
Introduction: Considering the high endemicity of typhoid in developing country and the feasibility of phage therapy for this disease, the study was planned to determine the genomic structure of bacteriophages specific to ‘Vi’ positive Salmonella Typhi. Aim: To use different molecular tools to charac...
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doaj-f8acfb3da9694125a4bb949a868388722020-11-25T02:52:32ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2019-10-011310BC14BC1710.7860/JCDR/2019/42189.13252A Genomic Study of Salmonella Typhi ‘Vi’ Specific Bacteriophages: Futuristic Approach in TherapeuticsPratibha Gavel0Sujit Bharti1Gopal Nath2Ragini Srivastava3Assistant Professor, Department of Biochemistry, Homi Bhabha Cancer Hospital, Varanasi, Uttar Pradesh, India.Consultant Microbiologist and Infection Control Officer, Department of Microbiology, Galaxy Hospital, Varanasi, Uttar Pradesh, India.Professor, Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.Professor, Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.Introduction: Considering the high endemicity of typhoid in developing country and the feasibility of phage therapy for this disease, the study was planned to determine the genomic structure of bacteriophages specific to ‘Vi’ positive Salmonella Typhi. Aim: To use different molecular tools to characterise Salmonella Typhi ‘Vi’ specific bacteriophages genotypically. Materials and Methods: Salmonella Typhi ‘Vi’ specific bacteriophages were isolated and propagated. Phage DNA was extracted and analysed by spectrophotometry, agarose gel electrophoresis and restriction enzyme digestion was done. Results: In the present study, two bacteriophages targeted against ‘Vi’ positive Salmonella Typhi were isolated. DNA of the phages was extracted for molecular characterisation. On restriction analysis, both the phages were found to be of similar pattern, suggesting that they were genetically similar, although they had different plaque morphologies. Conclusion: The present study may be helpful in genomic characterisation of Salmonella Typhi ‘Vi’ specific bacteriophages for their future use in therapeutics.https://jcdr.net/articles/PDF/13252/42189_CE[NG]_F(SL)_PF1(AG_SHU)_PFA(SHU)_PB(AG_SHU)_PN(SL).pdfagarose gel electrophoresisgenomic structurephage therapyrestriction digestionspectrophotometry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pratibha Gavel Sujit Bharti Gopal Nath Ragini Srivastava |
spellingShingle |
Pratibha Gavel Sujit Bharti Gopal Nath Ragini Srivastava A Genomic Study of Salmonella Typhi ‘Vi’ Specific Bacteriophages: Futuristic Approach in Therapeutics Journal of Clinical and Diagnostic Research agarose gel electrophoresis genomic structure phage therapy restriction digestion spectrophotometry |
author_facet |
Pratibha Gavel Sujit Bharti Gopal Nath Ragini Srivastava |
author_sort |
Pratibha Gavel |
title |
A Genomic Study of Salmonella Typhi ‘Vi’ Specific Bacteriophages: Futuristic Approach in Therapeutics |
title_short |
A Genomic Study of Salmonella Typhi ‘Vi’ Specific Bacteriophages: Futuristic Approach in Therapeutics |
title_full |
A Genomic Study of Salmonella Typhi ‘Vi’ Specific Bacteriophages: Futuristic Approach in Therapeutics |
title_fullStr |
A Genomic Study of Salmonella Typhi ‘Vi’ Specific Bacteriophages: Futuristic Approach in Therapeutics |
title_full_unstemmed |
A Genomic Study of Salmonella Typhi ‘Vi’ Specific Bacteriophages: Futuristic Approach in Therapeutics |
title_sort |
genomic study of salmonella typhi ‘vi’ specific bacteriophages: futuristic approach in therapeutics |
publisher |
JCDR Research and Publications Private Limited |
series |
Journal of Clinical and Diagnostic Research |
issn |
2249-782X 0973-709X |
publishDate |
2019-10-01 |
description |
Introduction: Considering the high endemicity of typhoid in developing country and the feasibility of phage therapy for this disease, the study was planned to determine the genomic structure of bacteriophages specific to ‘Vi’ positive Salmonella Typhi. Aim: To use different molecular tools to characterise Salmonella Typhi ‘Vi’ specific bacteriophages genotypically. Materials and Methods: Salmonella Typhi ‘Vi’ specific bacteriophages were isolated and propagated. Phage DNA was extracted and analysed by spectrophotometry, agarose gel electrophoresis and restriction enzyme digestion was done. Results: In the present study, two bacteriophages targeted against ‘Vi’ positive Salmonella Typhi were isolated. DNA of the phages was extracted for molecular characterisation. On restriction analysis, both the phages were found to be of similar pattern, suggesting that they were genetically similar, although they had different plaque morphologies. Conclusion: The present study may be helpful in genomic characterisation of Salmonella Typhi ‘Vi’ specific bacteriophages for their future use in therapeutics. |
topic |
agarose gel electrophoresis genomic structure phage therapy restriction digestion spectrophotometry |
url |
https://jcdr.net/articles/PDF/13252/42189_CE[NG]_F(SL)_PF1(AG_SHU)_PFA(SHU)_PB(AG_SHU)_PN(SL).pdf |
work_keys_str_mv |
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