Development of Food-Raw-Material-Based Nutrient Media for Submerged Cultivation of Cholera Vibrio Strains

• Main Authors: A. K. Nikiforov, M. V. Antonycheva, O. A. Volokh, S. A. Eremin, M. N. Kireev, I. M. Zhulidov, Yu. A. Aleshina, N. G. Avdeeva, N. I. Vakhrushina, K. I. Kholmatov
• Format: Article
• Language: Russian
• Published: Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” 2015-03-01
• Series: Проблемы особо опасных инфекций
• Subjects: питательная среда; гидролизат фибрина; автолизат пекарских дрожжей; вакцина холерная; глубинное культивирование; nutrient medium; fibrin hydrolyzate; bakery yeast autolyzate; cholera vaccine; submerged cultivation
• Online Access: https://journal.microbe.ru/jour/article/view/213

Carried out has been comparative assessment between the performances of liquid nutrient pancreatic fibrin overcook-based and bakery yeast autolyzate-based media and conventionally used in manufacturing of cholera vaccine media for submerged cultivation of Vibrio cholerae 569B and V. cholerae M-41 strains. Results of investigation of media quality biological predictors (morphological and biochemical property stability, efficacy of biomass and protective antigen accumulation), which are fibrin hydrolyzate and bakery yeast autolyzate-based, suggest the possibility of using them for the production of cholera vaccine. Deployment of inedible raw material-based media in manufacturing of cholera vaccine is a prospective technology in view of reduction of medical-prophylactic preparation costs. Moreover it allows for solving the problem of protein waste-product disposal, which is generated in the process of anti-rabies immunoglobulin manufacturing, thus decreasing ecological impact on the environment.