Elicitation of Antimicrobial Active Compounds by <i>Streptomyces</i>-Fungus Co-Cultures
The bacteria of the genus <i>Streptomyces</i> and Basidiomycete fungi harbor many biosynthetic gene clusters (BGCs) that are at the origin of many bioactive molecules with medical or industrial interests. Nevertheless, most BGCs do not express in standard lab growth conditions, preventin...
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doaj-f8d4a63c004d48a29aaf6adeb4de16312021-01-16T00:03:46ZengMDPI AGMicroorganisms2076-26072021-01-01917817810.3390/microorganisms9010178Elicitation of Antimicrobial Active Compounds by <i>Streptomyces</i>-Fungus Co-CulturesMatthieu Nicault0Ali Zaiter1Stéphane Dumarcay2Patrick Chaimbault3Eric Gelhaye4Pierre Leblond5Cyril Bontemps6Université de Lorraine, INRAE, DynAMic, F-54000 Nancy, FranceUniversité de Lorraine, LCP-A2MC, F-57000 Metz, FranceUniversité de Lorraine, INRAE, LERMAB, F-54000 Nancy, FranceUniversité de Lorraine, LCP-A2MC, F-57000 Metz, FranceUniversité de Lorraine, INRAE, IAM, F-54000 Nancy, FranceUniversité de Lorraine, INRAE, DynAMic, F-54000 Nancy, FranceUniversité de Lorraine, INRAE, DynAMic, F-54000 Nancy, FranceThe bacteria of the genus <i>Streptomyces</i> and Basidiomycete fungi harbor many biosynthetic gene clusters (BGCs) that are at the origin of many bioactive molecules with medical or industrial interests. Nevertheless, most BGCs do not express in standard lab growth conditions, preventing the full metabolic potential of these organisms from being exploited. Because it generates biotic cues encountered during natural growth conditions, co-culture is a means to elicit such cryptic compounds. In this study, we explored 72 different <i>Streptomyces</i>-fungus interaction zones (SFIZs) generated during the co-culture of eight <i>Streptomyces</i> and nine fungi. Two SFIZs were selected because they showed an elicitation of anti-bacterial activity compared to mono-cultures. The study of these SFIZs showed that co-culture had a strong impact on the metabolic expression of each partner and enabled the expression of specific compounds. These results show that mimicking the biotic interactions present in this ecological niche is a promising avenue of research to explore the metabolic capacities of <i>Streptomyces</i> and fungi.https://www.mdpi.com/2076-2607/9/1/178<i>Streptomyces</i>fungusco-culturespecialized metabolismbiosynthetic gene clusterantimicrobial agents |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Matthieu Nicault Ali Zaiter Stéphane Dumarcay Patrick Chaimbault Eric Gelhaye Pierre Leblond Cyril Bontemps |
spellingShingle |
Matthieu Nicault Ali Zaiter Stéphane Dumarcay Patrick Chaimbault Eric Gelhaye Pierre Leblond Cyril Bontemps Elicitation of Antimicrobial Active Compounds by <i>Streptomyces</i>-Fungus Co-Cultures Microorganisms <i>Streptomyces</i> fungus co-culture specialized metabolism biosynthetic gene cluster antimicrobial agents |
author_facet |
Matthieu Nicault Ali Zaiter Stéphane Dumarcay Patrick Chaimbault Eric Gelhaye Pierre Leblond Cyril Bontemps |
author_sort |
Matthieu Nicault |
title |
Elicitation of Antimicrobial Active Compounds by <i>Streptomyces</i>-Fungus Co-Cultures |
title_short |
Elicitation of Antimicrobial Active Compounds by <i>Streptomyces</i>-Fungus Co-Cultures |
title_full |
Elicitation of Antimicrobial Active Compounds by <i>Streptomyces</i>-Fungus Co-Cultures |
title_fullStr |
Elicitation of Antimicrobial Active Compounds by <i>Streptomyces</i>-Fungus Co-Cultures |
title_full_unstemmed |
Elicitation of Antimicrobial Active Compounds by <i>Streptomyces</i>-Fungus Co-Cultures |
title_sort |
elicitation of antimicrobial active compounds by <i>streptomyces</i>-fungus co-cultures |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2021-01-01 |
description |
The bacteria of the genus <i>Streptomyces</i> and Basidiomycete fungi harbor many biosynthetic gene clusters (BGCs) that are at the origin of many bioactive molecules with medical or industrial interests. Nevertheless, most BGCs do not express in standard lab growth conditions, preventing the full metabolic potential of these organisms from being exploited. Because it generates biotic cues encountered during natural growth conditions, co-culture is a means to elicit such cryptic compounds. In this study, we explored 72 different <i>Streptomyces</i>-fungus interaction zones (SFIZs) generated during the co-culture of eight <i>Streptomyces</i> and nine fungi. Two SFIZs were selected because they showed an elicitation of anti-bacterial activity compared to mono-cultures. The study of these SFIZs showed that co-culture had a strong impact on the metabolic expression of each partner and enabled the expression of specific compounds. These results show that mimicking the biotic interactions present in this ecological niche is a promising avenue of research to explore the metabolic capacities of <i>Streptomyces</i> and fungi. |
topic |
<i>Streptomyces</i> fungus co-culture specialized metabolism biosynthetic gene cluster antimicrobial agents |
url |
https://www.mdpi.com/2076-2607/9/1/178 |
work_keys_str_mv |
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