The Effects of Cleistanthoside A Tetraacetate Synthesis on Acute Toxicity and Bone Marrow Micronucleus in ICR Mice

• Main Authors: Pranom PUCHADAPIROM, Wanwisa HIMAKHUN, Patoomratana TUCHINDA, Lakana HIMAKOUN, Thidarat KOOMSANG, Prasit SUWANNALERT
• Format: Article
• Language: English
• Published: Walailak University 2015-07-01
• Series: Walailak Journal of Science and Technology
• Subjects: Cleistanthoside A tetraacetate; toxicity; micronucleus; Phyllanthus spp.
• Online Access: http://wjst.wu.ac.th/index.php/wjst/article/view/1414
• ISSN: 1686-3933; 2228-835X

Phyllanthus taxodiifolius Beille is used in traditional medicine in tropical and subtropical areas. Although it has been used as a folk medicine for a long time, studies on its safety have been limited. In this study, the bone marrow micronucleus and oral toxicity of Cleistanthoside A tetraacetate, a modified arylnaphthalide lignan of Cleistanthoside A from the P. taxodiifolius Beille, were evaluated. Imprinting control region (ICR) female mice were orally administered Cleistanthoside A tetraacetate at doses of 250, 500 and 1,000 mg/kg body weight (BW). Signs of toxicity, bone marrow micronucleus, clinical blood chemistry, and histopathological findings were determined after treatment. No mortality was observed in any of the groups. A significant increase in numbers of bone marrow micronucleus, marked elevation of alkaline phosphatase (ALP), blood urea nitrogen (BUN), and creatinine (CRE) were observed in the mice administered with Cleistanthoside A tetraacetate 1,000 mg/kg BW. These results also correlated with the histopathological scoring of liver and kidney cells in ICR mice. The mice administered with Cleistanthoside A tetraacetate 1,000 mg/kg BW had high toxicity in the liver and kidneys. Long term or chronic toxicity should be further studied for safety.