Selective release of circRNAs in platelet-derived extracellular vesicles
Circular RNAs (circRNAs) are a novel class of noncoding RNAs present in all eukaryotic cells investigated so far and generated by a special mode of alternative splicing of pre-mRNAs. Thereby, single exons, or multiple adjacent and spliced exons, are released in a circular form. CircRNAs are cell-typ...
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Online Access: | http://dx.doi.org/10.1080/20013078.2018.1424473 |
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doaj-f9102faa45c94a298eed71fe8c9b52252020-11-24T21:09:43ZengTaylor & Francis GroupJournal of Extracellular Vesicles2001-30782018-12-017110.1080/20013078.2018.14244731424473Selective release of circRNAs in platelet-derived extracellular vesiclesChristian Preußer0Lee-Hsueh Hung1Tim Schneider2Silke Schreiner3Martin Hardt4Anna Moebus5Sentot Santoso6Albrecht Bindereif7Justus Liebig University of GiessenJustus Liebig University of GiessenJustus Liebig University of GiessenJustus Liebig University of GiessenJustus Liebig University of GiessenJustus Liebig University of GiessenJustus Liebig University of GiessenJustus Liebig University of GiessenCircular RNAs (circRNAs) are a novel class of noncoding RNAs present in all eukaryotic cells investigated so far and generated by a special mode of alternative splicing of pre-mRNAs. Thereby, single exons, or multiple adjacent and spliced exons, are released in a circular form. CircRNAs are cell-type specifically expressed, are unusually stable, and can be found in various body fluids such as blood and saliva. Here we analysed circRNAs and the corresponding linear splice isoforms from human platelets, where circRNAs are particularly abundant, compared with other hematopoietic cell types. In addition, we isolated extracellular vesicles from purified and in vitro activated human platelets, using density-gradient centrifugation, followed by RNA-seq analysis for circRNA detection. We could demonstrate that circRNAs are packaged and released within both types of vesicles (microvesicles and exosomes) derived from platelets. Interestingly, we observed a selective release of circRNAs into the vesicles, suggesting a specific sorting mechanism. In sum, circRNAs represent yet another class of extracellular RNAs that circulate in the body and may be involved in signalling pathways. Since platelets are essential for central physiological processes such as haemostasis, wound healing, inflammation and cancer metastasis, these findings should greatly extend the potential of circRNAs as prognostic and diagnostic biomarkers.http://dx.doi.org/10.1080/20013078.2018.1424473CircRNAsextracellular vesiclesexosomesmicrovesiclesplatelets |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Christian Preußer Lee-Hsueh Hung Tim Schneider Silke Schreiner Martin Hardt Anna Moebus Sentot Santoso Albrecht Bindereif |
spellingShingle |
Christian Preußer Lee-Hsueh Hung Tim Schneider Silke Schreiner Martin Hardt Anna Moebus Sentot Santoso Albrecht Bindereif Selective release of circRNAs in platelet-derived extracellular vesicles Journal of Extracellular Vesicles CircRNAs extracellular vesicles exosomes microvesicles platelets |
author_facet |
Christian Preußer Lee-Hsueh Hung Tim Schneider Silke Schreiner Martin Hardt Anna Moebus Sentot Santoso Albrecht Bindereif |
author_sort |
Christian Preußer |
title |
Selective release of circRNAs in platelet-derived extracellular vesicles |
title_short |
Selective release of circRNAs in platelet-derived extracellular vesicles |
title_full |
Selective release of circRNAs in platelet-derived extracellular vesicles |
title_fullStr |
Selective release of circRNAs in platelet-derived extracellular vesicles |
title_full_unstemmed |
Selective release of circRNAs in platelet-derived extracellular vesicles |
title_sort |
selective release of circrnas in platelet-derived extracellular vesicles |
publisher |
Taylor & Francis Group |
series |
Journal of Extracellular Vesicles |
issn |
2001-3078 |
publishDate |
2018-12-01 |
description |
Circular RNAs (circRNAs) are a novel class of noncoding RNAs present in all eukaryotic cells investigated so far and generated by a special mode of alternative splicing of pre-mRNAs. Thereby, single exons, or multiple adjacent and spliced exons, are released in a circular form. CircRNAs are cell-type specifically expressed, are unusually stable, and can be found in various body fluids such as blood and saliva. Here we analysed circRNAs and the corresponding linear splice isoforms from human platelets, where circRNAs are particularly abundant, compared with other hematopoietic cell types. In addition, we isolated extracellular vesicles from purified and in vitro activated human platelets, using density-gradient centrifugation, followed by RNA-seq analysis for circRNA detection. We could demonstrate that circRNAs are packaged and released within both types of vesicles (microvesicles and exosomes) derived from platelets. Interestingly, we observed a selective release of circRNAs into the vesicles, suggesting a specific sorting mechanism. In sum, circRNAs represent yet another class of extracellular RNAs that circulate in the body and may be involved in signalling pathways. Since platelets are essential for central physiological processes such as haemostasis, wound healing, inflammation and cancer metastasis, these findings should greatly extend the potential of circRNAs as prognostic and diagnostic biomarkers. |
topic |
CircRNAs extracellular vesicles exosomes microvesicles platelets |
url |
http://dx.doi.org/10.1080/20013078.2018.1424473 |
work_keys_str_mv |
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1716757616048209920 |