Identification of microRNAs and mRNAs associated with multidrug resistance of human laryngeal cancer Hep-2 cells

• Main Authors: Wanzhong Yin, Ping Wang, Xin Wang, Wenzhi Song, Xiangyan Cui, Hong Yu, Wei Zhu
• Format: Article
• Language: English
• Published: Associação Brasileira de Divulgação Científica 2013-12-01
• Series: Brazilian Journal of Medical and Biological Research
• Subjects: Multidrug resistance; Laryngeal cancer; Hep-2 cells; mRNA; microRNA
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013005031662
• ISSN: 0100-879X; 1414-431X

Multidrug resistance (MDR) poses a serious impediment to the success of chemotherapy for laryngeal cancer. To identify microRNAs and mRNAs associated with MDR of human laryngeal cancer Hep-2 cells, we developed a multidrug-resistant human laryngeal cancer subline, designated Hep-2/v, by exposing Hep-2 cells to stepwise increasing concentrations of vincristine (0.02-0.96&#8197;&#181;M). Microarray assays were performed to compare the microRNA and mRNA expression profiles of Hep-2 and Hep-2/v cells. Compared to Hep-2 cells, Hep-2/v cells were more resistant to chemotherapy drugs (&#8764;45-fold more resistant to vincristine, 5.1-fold more resistant to cisplatin, and 5.6-fold more resistant to 5-fluorouracil) and had a longer doubling time (42.33&#177;1.76 vs 28.75&#177;1.12&#8197;h, P<0.05), higher percentage of cells in G0/G1 phase (80.98&#177;0.52 vs 69.14&#177;0.89, P<0.05), increased efflux of rhodamine 123 (95.97&#177;0.56 vs 12.40&#177;0.44%, P<0.01), and up-regulated MDR1 expression. A total of 7 microRNAs and 605 mRNAs were differentially expressed between the two cell types. Of the differentially expressed mRNAs identified, regulator of G-protein signaling 10, high-temperature requirement protein A1, and nuclear protein 1 were found to be the putative targets of the differentially expressed microRNAs identified. These findings may open a new avenue for clarifying the mechanisms responsible for MDR in laryngeal cancer.