Paeoniflorin Antagonizes TNF-α-Induced L929 Fibroblastoma Cells Apoptosis by Inhibiting NF-κBp65 Activation
Paeoniflorin (PF) is one of the main pharmacodynamic components of Paeonia suffruticosa Andr , which has a significant anti-inflammatory effect on rheumatoid arthritis (RA), with a mechanism related to the tumor necrosis factor α (TNF-α). The aim of the present study was to investigate the role of P...
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Online Access: | https://doi.org/10.1177/1559325818774977 |
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doaj-f922b4db7abe4077bf89f60c58720dcf2020-11-25T03:32:32ZengSAGE PublishingDose-Response1559-32582018-06-011610.1177/1559325818774977Paeoniflorin Antagonizes TNF-α-Induced L929 Fibroblastoma Cells Apoptosis by Inhibiting NF-κBp65 ActivationXiaoyu Lai0Jing Wei1Xinghong Ding2 Institute of Hematology, Zhejiang University, Hangzhou, People’s Republic of China College of Life Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People’s Republic of China College of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People’s Republic of ChinaPaeoniflorin (PF) is one of the main pharmacodynamic components of Paeonia suffruticosa Andr , which has a significant anti-inflammatory effect on rheumatoid arthritis (RA), with a mechanism related to the tumor necrosis factor α (TNF-α). The aim of the present study was to investigate the role of PF in the apoptosis and expression of NF-κBp65 of L929 fibroblastoma cells induced by TNF-α. Our results showed that different concentrations of PF can significantly reduce the growth inhibition of L929 cells. Moreover, morphological observations, Hoechst 33342 staining, and flow cytometry detection of apoptosis showed that PF can significantly attenuate the TNF-α-induced apoptosis in a dose-dependent manner. Western blot analysis revealed that TNF-α induced the activation of NF-κBp65, whereas PF treatment had a marked dose-dependent suppression on it, which indicates that its action might be associated with inhibiting NF-κB signaling pathway. These results show that PF exerts a beneficial effect on L929 cells to prevent TNF-α-induced apoptosis and expression of NF-κBp65, which would be helpful to clarify its role in the treatment of RA.https://doi.org/10.1177/1559325818774977 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaoyu Lai Jing Wei Xinghong Ding |
spellingShingle |
Xiaoyu Lai Jing Wei Xinghong Ding Paeoniflorin Antagonizes TNF-α-Induced L929 Fibroblastoma Cells Apoptosis by Inhibiting NF-κBp65 Activation Dose-Response |
author_facet |
Xiaoyu Lai Jing Wei Xinghong Ding |
author_sort |
Xiaoyu Lai |
title |
Paeoniflorin Antagonizes TNF-α-Induced L929 Fibroblastoma Cells Apoptosis by Inhibiting NF-κBp65 Activation |
title_short |
Paeoniflorin Antagonizes TNF-α-Induced L929 Fibroblastoma Cells Apoptosis by Inhibiting NF-κBp65 Activation |
title_full |
Paeoniflorin Antagonizes TNF-α-Induced L929 Fibroblastoma Cells Apoptosis by Inhibiting NF-κBp65 Activation |
title_fullStr |
Paeoniflorin Antagonizes TNF-α-Induced L929 Fibroblastoma Cells Apoptosis by Inhibiting NF-κBp65 Activation |
title_full_unstemmed |
Paeoniflorin Antagonizes TNF-α-Induced L929 Fibroblastoma Cells Apoptosis by Inhibiting NF-κBp65 Activation |
title_sort |
paeoniflorin antagonizes tnf-α-induced l929 fibroblastoma cells apoptosis by inhibiting nf-κbp65 activation |
publisher |
SAGE Publishing |
series |
Dose-Response |
issn |
1559-3258 |
publishDate |
2018-06-01 |
description |
Paeoniflorin (PF) is one of the main pharmacodynamic components of Paeonia suffruticosa Andr , which has a significant anti-inflammatory effect on rheumatoid arthritis (RA), with a mechanism related to the tumor necrosis factor α (TNF-α). The aim of the present study was to investigate the role of PF in the apoptosis and expression of NF-κBp65 of L929 fibroblastoma cells induced by TNF-α. Our results showed that different concentrations of PF can significantly reduce the growth inhibition of L929 cells. Moreover, morphological observations, Hoechst 33342 staining, and flow cytometry detection of apoptosis showed that PF can significantly attenuate the TNF-α-induced apoptosis in a dose-dependent manner. Western blot analysis revealed that TNF-α induced the activation of NF-κBp65, whereas PF treatment had a marked dose-dependent suppression on it, which indicates that its action might be associated with inhibiting NF-κB signaling pathway. These results show that PF exerts a beneficial effect on L929 cells to prevent TNF-α-induced apoptosis and expression of NF-κBp65, which would be helpful to clarify its role in the treatment of RA. |
url |
https://doi.org/10.1177/1559325818774977 |
work_keys_str_mv |
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