Drug Resistance Pattern of Toxigenic Clostridium difficileIsolated from Patients with Diarrhea to Conventional Therapeutic Drugs with Chronic

• Main Authors: Mohsen Zargar, Mohammad Reza Ghadir, Ali Javadi, Abbass Morovvati, Fariba Dastjani Farahani
• Format: Article
• Language: fas
• Published: Qom University of Medical Sciences 2020-02-01
• Series: Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum
• Subjects: clostridium difficile; diarrhea; toxin a; toxin b; polymerase chain reaction; drug resistance.
• Online Access: http://journal.muq.ac.ir/article-1-2635-en.html
• ISSN: 1735-7799; 2008-1375

Background and Objectives: Clostridium difficile is a gram-positive obligate anaerobic bacillus. Accurate and rapid diagnosis of this bacterium as well as assessment of its drug resistance pattern are one of the important issues in controlling infections caused by C. difficile. The purpose of the present study was to detect toxicogenic C. difficile and to evaluate drug resistance pattern of these strains to routine therapeutic drugs.   Methods: A total of 68 samples were collected from patients hospitalized in hospitals of Qom province. All samples were rapidly cultured in the specialized CCFA Agar medium.After these procedures, the bacteria were used for DNA extraction and then PCR was performed on the cdd3 gene for definitive detection of the bacteria. Finally, the presence of A and B toxins’ genes was determined by PCR and resistance to metronidazole, vancomycin, and clindamycin, was determined by agar dilution method. Chi-square test was used to analyze the results.   Results: In this study, 6 isolates had A and B toxins, the highest antibiotic resistance was against clindamycin (45.45%) and metronidazole (36.36%), and all the isolates, were sensitive to vancomycin.   Conclusion: Drug resistance pattern in the obtained strains still introduces vancomycin as the treatment of choice.