SARS-CoV-2 Entry Factors: ACE2 and TMPRSS2 Are Expressed in Peri-Implantation Embryos and the Maternal–Fetal Interface
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread throughout the world, leading to large-scale population infection. Angiotensin-converting enzyme 2 (ACE2) is the receptor of both severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2. However, it is still cont...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2020-10-01
|
Series: | Engineering |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2095809920302162 |
id |
doaj-f927d149163740c7b8615feb0091d403 |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wei Chen Peng Yuan Ming Yang Zhiqiang Yan Siming Kong Jie Yan Xixi Liu Yidong Chen Jie Qiao Liying Yan |
spellingShingle |
Wei Chen Peng Yuan Ming Yang Zhiqiang Yan Siming Kong Jie Yan Xixi Liu Yidong Chen Jie Qiao Liying Yan SARS-CoV-2 Entry Factors: ACE2 and TMPRSS2 Are Expressed in Peri-Implantation Embryos and the Maternal–Fetal Interface Engineering SARS-CoV-2 ACE2 Vertical transmission Placenta Peri-implantation |
author_facet |
Wei Chen Peng Yuan Ming Yang Zhiqiang Yan Siming Kong Jie Yan Xixi Liu Yidong Chen Jie Qiao Liying Yan |
author_sort |
Wei Chen |
title |
SARS-CoV-2 Entry Factors: ACE2 and TMPRSS2 Are Expressed in Peri-Implantation Embryos and the Maternal–Fetal Interface |
title_short |
SARS-CoV-2 Entry Factors: ACE2 and TMPRSS2 Are Expressed in Peri-Implantation Embryos and the Maternal–Fetal Interface |
title_full |
SARS-CoV-2 Entry Factors: ACE2 and TMPRSS2 Are Expressed in Peri-Implantation Embryos and the Maternal–Fetal Interface |
title_fullStr |
SARS-CoV-2 Entry Factors: ACE2 and TMPRSS2 Are Expressed in Peri-Implantation Embryos and the Maternal–Fetal Interface |
title_full_unstemmed |
SARS-CoV-2 Entry Factors: ACE2 and TMPRSS2 Are Expressed in Peri-Implantation Embryos and the Maternal–Fetal Interface |
title_sort |
sars-cov-2 entry factors: ace2 and tmprss2 are expressed in peri-implantation embryos and the maternal–fetal interface |
publisher |
Elsevier |
series |
Engineering |
issn |
2095-8099 |
publishDate |
2020-10-01 |
description |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread throughout the world, leading to large-scale population infection. Angiotensin-converting enzyme 2 (ACE2) is the receptor of both severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2. However, it is still controversial whether vertical transmission exists. In order to investigate the potential risk of SARS-CoV-2 vertical transmission, we explored ACE2 and TMPRSS2 (encoding transmembrane protease serine 2) expression patterns in peri-implantation embryos and the maternal–fetal interface using previously published single-cell transcriptome data. The results showed that day 6 (D6) trophectoderm (TE) cells in peri-implantation embryos, as well as syncytiotrophoblast (STB) at 8 weeks of gestation (STB_8W) and extravillous trophoblast (EVT) cells at 24 weeks of gestation (EVT_24W) in the maternal–fetal interface, strongly co-expressed ACE2 and TMPRSS2, indicating a SARS-CoV-2 infection susceptibility. The ACE2 positive-expressing cells in the three cell types mentioned above were found to share common characteristics, which were involved in autophagy and immune-related processes. ACE2 showed no gender bias in post-implantation embryos but showed a significant gender difference in D6_TE, D6 primitive endoderm (PE) cells, and ACE2 positive-expressing STBs. These findings suggest that there may be different SARS-CoV-2 infection susceptibilities of D6 embryos of different genders and during the gestation of different genders. Our results reveal potential SARS-CoV-2 infection risks during embryo transfer, peri-implantation embryo development, and gestation. |
topic |
SARS-CoV-2 ACE2 Vertical transmission Placenta Peri-implantation |
url |
http://www.sciencedirect.com/science/article/pii/S2095809920302162 |
work_keys_str_mv |
AT weichen sarscov2entryfactorsace2andtmprss2areexpressedinperiimplantationembryosandthematernalfetalinterface AT pengyuan sarscov2entryfactorsace2andtmprss2areexpressedinperiimplantationembryosandthematernalfetalinterface AT mingyang sarscov2entryfactorsace2andtmprss2areexpressedinperiimplantationembryosandthematernalfetalinterface AT zhiqiangyan sarscov2entryfactorsace2andtmprss2areexpressedinperiimplantationembryosandthematernalfetalinterface AT simingkong sarscov2entryfactorsace2andtmprss2areexpressedinperiimplantationembryosandthematernalfetalinterface AT jieyan sarscov2entryfactorsace2andtmprss2areexpressedinperiimplantationembryosandthematernalfetalinterface AT xixiliu sarscov2entryfactorsace2andtmprss2areexpressedinperiimplantationembryosandthematernalfetalinterface AT yidongchen sarscov2entryfactorsace2andtmprss2areexpressedinperiimplantationembryosandthematernalfetalinterface AT jieqiao sarscov2entryfactorsace2andtmprss2areexpressedinperiimplantationembryosandthematernalfetalinterface AT liyingyan sarscov2entryfactorsace2andtmprss2areexpressedinperiimplantationembryosandthematernalfetalinterface |
_version_ |
1724388587930124288 |
spelling |
doaj-f927d149163740c7b8615feb0091d4032020-12-09T04:15:15ZengElsevierEngineering2095-80992020-10-0161011621169SARS-CoV-2 Entry Factors: ACE2 and TMPRSS2 Are Expressed in Peri-Implantation Embryos and the Maternal–Fetal InterfaceWei Chen0Peng Yuan1Ming Yang2Zhiqiang Yan3Siming Kong4Jie Yan5Xixi Liu6Yidong Chen7Jie Qiao8Liying Yan9Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China; Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Peking University Third Hospital, Beijing 100191, China; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; Peking–Tsinghua Center for Life Sciences, Peking University, Beijing 100871, ChinaCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China; Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Peking University Third Hospital, Beijing 100191, ChinaCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China; Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Peking University Third Hospital, Beijing 100191, China; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; Peking–Tsinghua Center for Life Sciences, Peking University, Beijing 100871, ChinaCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China; Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Peking University Third Hospital, Beijing 100191, China; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; Peking–Tsinghua Center for Life Sciences, Peking University, Beijing 100871, ChinaCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China; Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Peking University Third Hospital, Beijing 100191, China; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; Peking–Tsinghua Center for Life Sciences, Peking University, Beijing 100871, ChinaCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China; Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Peking University Third Hospital, Beijing 100191, China; National Clinical Research Center for Obstetrics and Gynecology, Beijing 100191, China; Beijing Advanced Innovation Center for Genomics, Beijing 100871, China; Research Units of Comprehensive Diagnosis and Treatment of Oocyte Maturation Arrest, Beijing 100191, ChinaCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Peking University Third Hospital, Beijing 100191, China; National Clinical Research Center for Obstetrics and Gynecology, Beijing 100191, ChinaCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China; Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Peking University Third Hospital, Beijing 100191, China; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; Peking–Tsinghua Center for Life Sciences, Peking University, Beijing 100871, ChinaCenter for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China; Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Peking University Third Hospital, Beijing 100191, China; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; Peking–Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China; National Clinical Research Center for Obstetrics and Gynecology, Beijing 100191, China; Beijing Advanced Innovation Center for Genomics, Beijing 100871, China; Research Units of Comprehensive Diagnosis and Treatment of Oocyte Maturation Arrest, Beijing 100191, China; Corresponding authors.Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China; Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Peking University Third Hospital, Beijing 100191, China; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; National Clinical Research Center for Obstetrics and Gynecology, Beijing 100191, China; Beijing Advanced Innovation Center for Genomics, Beijing 100871, China; Research Units of Comprehensive Diagnosis and Treatment of Oocyte Maturation Arrest, Beijing 100191, China; Corresponding authors.Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread throughout the world, leading to large-scale population infection. Angiotensin-converting enzyme 2 (ACE2) is the receptor of both severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2. However, it is still controversial whether vertical transmission exists. In order to investigate the potential risk of SARS-CoV-2 vertical transmission, we explored ACE2 and TMPRSS2 (encoding transmembrane protease serine 2) expression patterns in peri-implantation embryos and the maternal–fetal interface using previously published single-cell transcriptome data. The results showed that day 6 (D6) trophectoderm (TE) cells in peri-implantation embryos, as well as syncytiotrophoblast (STB) at 8 weeks of gestation (STB_8W) and extravillous trophoblast (EVT) cells at 24 weeks of gestation (EVT_24W) in the maternal–fetal interface, strongly co-expressed ACE2 and TMPRSS2, indicating a SARS-CoV-2 infection susceptibility. The ACE2 positive-expressing cells in the three cell types mentioned above were found to share common characteristics, which were involved in autophagy and immune-related processes. ACE2 showed no gender bias in post-implantation embryos but showed a significant gender difference in D6_TE, D6 primitive endoderm (PE) cells, and ACE2 positive-expressing STBs. These findings suggest that there may be different SARS-CoV-2 infection susceptibilities of D6 embryos of different genders and during the gestation of different genders. Our results reveal potential SARS-CoV-2 infection risks during embryo transfer, peri-implantation embryo development, and gestation.http://www.sciencedirect.com/science/article/pii/S2095809920302162SARS-CoV-2ACE2Vertical transmissionPlacentaPeri-implantation |