Phase separation by the polyhomeotic sterile alpha motif compartmentalizes Polycomb Group proteins and enhances their activity

The conserved SAM motif of Polycomb Repressive Complex 1 subunit Ph has been shown to play an important role in chromatin organization. Here, the authors study the effect of Ph SAM on chromatin in vitro, showing that it induces the formation of concentrated, phase-separated condensates, which enhanc...

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Main Authors:	Elias Seif, Jin Joo Kang, Charles Sasseville, Olga Senkovich, Alexander Kaltashov, Elodie L. Boulier, Ibani Kapur, Chongwoo A. Kim, Nicole J. Francis
Format:	Article
Language:	English
Published:	Nature Publishing Group 2020-11-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-020-19435-z

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spelling	doaj-f93773e13b25400184a9e8d15645342f2021-05-11T08:29:55ZengNature Publishing GroupNature Communications2041-17232020-11-0111111910.1038/s41467-020-19435-zPhase separation by the polyhomeotic sterile alpha motif compartmentalizes Polycomb Group proteins and enhances their activityElias Seif0Jin Joo Kang1Charles Sasseville2Olga Senkovich3Alexander Kaltashov4Elodie L. Boulier5Ibani Kapur6Chongwoo A. Kim7Nicole J. Francis8Institut de recherches cliniques de MontréalInstitut de recherches cliniques de MontréalInstitut de recherches cliniques de MontréalDepartment of Biochemistry and Molecular Genetics, Midwestern UniversityInstitut de recherches cliniques de MontréalInstitut de recherches cliniques de MontréalInstitut de recherches cliniques de MontréalDepartment of Biochemistry and Molecular Genetics, Midwestern UniversityInstitut de recherches cliniques de MontréalThe conserved SAM motif of Polycomb Repressive Complex 1 subunit Ph has been shown to play an important role in chromatin organization. Here, the authors study the effect of Ph SAM on chromatin in vitro, showing that it induces the formation of concentrated, phase-separated condensates, which enhance the ubiquitin ligase activity of PRC1.https://doi.org/10.1038/s41467-020-19435-z
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Elias Seif Jin Joo Kang Charles Sasseville Olga Senkovich Alexander Kaltashov Elodie L. Boulier Ibani Kapur Chongwoo A. Kim Nicole J. Francis
spellingShingle	Elias Seif Jin Joo Kang Charles Sasseville Olga Senkovich Alexander Kaltashov Elodie L. Boulier Ibani Kapur Chongwoo A. Kim Nicole J. Francis Phase separation by the polyhomeotic sterile alpha motif compartmentalizes Polycomb Group proteins and enhances their activity Nature Communications
author_facet	Elias Seif Jin Joo Kang Charles Sasseville Olga Senkovich Alexander Kaltashov Elodie L. Boulier Ibani Kapur Chongwoo A. Kim Nicole J. Francis
author_sort	Elias Seif
title	Phase separation by the polyhomeotic sterile alpha motif compartmentalizes Polycomb Group proteins and enhances their activity
title_short	Phase separation by the polyhomeotic sterile alpha motif compartmentalizes Polycomb Group proteins and enhances their activity
title_full	Phase separation by the polyhomeotic sterile alpha motif compartmentalizes Polycomb Group proteins and enhances their activity
title_fullStr	Phase separation by the polyhomeotic sterile alpha motif compartmentalizes Polycomb Group proteins and enhances their activity
title_full_unstemmed	Phase separation by the polyhomeotic sterile alpha motif compartmentalizes Polycomb Group proteins and enhances their activity
title_sort	phase separation by the polyhomeotic sterile alpha motif compartmentalizes polycomb group proteins and enhances their activity
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2020-11-01
description	The conserved SAM motif of Polycomb Repressive Complex 1 subunit Ph has been shown to play an important role in chromatin organization. Here, the authors study the effect of Ph SAM on chromatin in vitro, showing that it induces the formation of concentrated, phase-separated condensates, which enhance the ubiquitin ligase activity of PRC1.
url	https://doi.org/10.1038/s41467-020-19435-z
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Phase separation by the polyhomeotic sterile alpha motif compartmentalizes Polycomb Group proteins and enhances their activity

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