Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials

Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials

The purpose of this study was to develop the pathway of silk fibroin (SF) biopolymer surface induced cell membrane protein activation. Fibroblasts were used as an experimental model to evaluate the responses of cellular proteins induced by biopolymer material using a mass spectrometry-based profilin...

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Main Authors: Ming-Hui Yang, Shyng-Shiou Yuan, Tze-Wen Chung, Shiang-Bin Jong, Chi-Yu Lu, Wan-Chi Tsai, Wen-Cheng Chen, Po-Chiao Lin, Pei-Wen Chiang, Yu-Chang Tyan
Format: Article
Language:English
Published: Hindawi Limited 2014-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2014/209469
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spelling doaj-f9384420f1ce4234a8dadf1bac8499dd2020-11-24T23:32:13ZengHindawi LimitedBioMed Research International2314-61332314-61412014-01-01201410.1155/2014/209469209469Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by BiomaterialsMing-Hui Yang0Shyng-Shiou Yuan1Tze-Wen Chung2Shiang-Bin Jong3Chi-Yu Lu4Wan-Chi Tsai5Wen-Cheng Chen6Po-Chiao Lin7Pei-Wen Chiang8Yu-Chang Tyan9Instrument Technology Research Center, National Applied Research Laboratories, Hsinchu 300, TaiwanDepartment of Medical Research, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, TaiwanDepartment of Biomedical Engineering, National Yang-Ming University, Taipei 112, TaiwanDepartment of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, TaiwanDepartment of Biochemistry, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, TaiwanDepartment of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 807, TaiwanDepartment of Fiber and Composite Materials, College of Engineering, Feng Chia University, Taichung 407, TaiwanNational Sun Yat-Sen University-Kaohsiung Medical University Joint Research Center, Kaohsiung 804, TaiwanDepartment of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, TaiwanTranslational Research Center, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung 807, TaiwanThe purpose of this study was to develop the pathway of silk fibroin (SF) biopolymer surface induced cell membrane protein activation. Fibroblasts were used as an experimental model to evaluate the responses of cellular proteins induced by biopolymer material using a mass spectrometry-based profiling system. The surface was covered by multiwalled carbon nanotubes (CNTs) and SF to increase the surface area, enhance the adhesion of biopolymer, and promote the rate of cell proliferation. The amount of adhered fibroblasts on CNTs/SF electrodes of quartz crystal microbalance (QCM) greatly exceeded those on other surfaces. Moreover, analyzing differential protein expressions of adhered fibroblasts on the biopolymer surface by proteomic approaches indicated that CD44 may be a key protein. Through this study, utilization of mass spectrometry-based proteomics in evaluation of cell adhesion on biopolymer was proposed.http://dx.doi.org/10.1155/2014/209469
collection DOAJ
language English
format Article
sources DOAJ
author Ming-Hui Yang
Shyng-Shiou Yuan
Tze-Wen Chung
Shiang-Bin Jong
Chi-Yu Lu
Wan-Chi Tsai
Wen-Cheng Chen
Po-Chiao Lin
Pei-Wen Chiang
Yu-Chang Tyan
spellingShingle Ming-Hui Yang
Shyng-Shiou Yuan
Tze-Wen Chung
Shiang-Bin Jong
Chi-Yu Lu
Wan-Chi Tsai
Wen-Cheng Chen
Po-Chiao Lin
Pei-Wen Chiang
Yu-Chang Tyan
Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials
BioMed Research International
author_facet Ming-Hui Yang
Shyng-Shiou Yuan
Tze-Wen Chung
Shiang-Bin Jong
Chi-Yu Lu
Wan-Chi Tsai
Wen-Cheng Chen
Po-Chiao Lin
Pei-Wen Chiang
Yu-Chang Tyan
author_sort Ming-Hui Yang
title Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials
title_short Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials
title_full Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials
title_fullStr Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials
title_full_unstemmed Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials
title_sort characterization of silk fibroin modified surface: a proteomic view of cellular response proteins induced by biomaterials
publisher Hindawi Limited
series BioMed Research International
issn 2314-6133
2314-6141
publishDate 2014-01-01
description The purpose of this study was to develop the pathway of silk fibroin (SF) biopolymer surface induced cell membrane protein activation. Fibroblasts were used as an experimental model to evaluate the responses of cellular proteins induced by biopolymer material using a mass spectrometry-based profiling system. The surface was covered by multiwalled carbon nanotubes (CNTs) and SF to increase the surface area, enhance the adhesion of biopolymer, and promote the rate of cell proliferation. The amount of adhered fibroblasts on CNTs/SF electrodes of quartz crystal microbalance (QCM) greatly exceeded those on other surfaces. Moreover, analyzing differential protein expressions of adhered fibroblasts on the biopolymer surface by proteomic approaches indicated that CD44 may be a key protein. Through this study, utilization of mass spectrometry-based proteomics in evaluation of cell adhesion on biopolymer was proposed.
url http://dx.doi.org/10.1155/2014/209469
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