Impact of a weight loss and fitness intervention on exercise‐associated plasma oxylipin patterns in obese, insulin‐resistant, sedentary women
Abstract Very little is known about how metabolic health status, insulin resistance or metabolic challenges modulate the endocannabinoid (eCB) or polyunsaturated fatty acid (PUFA)‐derived oxylipin (OxL) lipid classes. To address these questions, plasma eCB and OxL concentrations were determined at r...
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doaj-f939dd25011c4530ad740512e06456372020-11-25T03:25:26ZengWileyPhysiological Reports2051-817X2020-09-01817n/an/a10.14814/phy2.14547Impact of a weight loss and fitness intervention on exercise‐associated plasma oxylipin patterns in obese, insulin‐resistant, sedentary womenDmitry Grapov0Oliver Fiehn1Caitlin Campbell2Carol J. Chandler3Dustin J. Burnett4Elaine C. Souza5Gretchen A. Casazza6Nancy L. Keim7Gary R. Hunter8Jose R. Fernandez9W. Timothy Garvey10Charles L. Hoppel11Mary‐Ellen Harper12John W. Newman13Sean H. Adams14CDS‐Creative Data Solutions Davis CA USAWest Coast Metabolomics Center University of California Davis CA USAUnited States Department of Agriculture‐Agricultural Research Service Western Human Nutrition Research Center Davis CA USAUnited States Department of Agriculture‐Agricultural Research Service Western Human Nutrition Research Center Davis CA USAUnited States Department of Agriculture‐Agricultural Research Service Western Human Nutrition Research Center Davis CA USAUnited States Department of Agriculture‐Agricultural Research Service Western Human Nutrition Research Center Davis CA USADepartment of Kinesiology California State University Sacramento CA USAUnited States Department of Agriculture‐Agricultural Research Service Western Human Nutrition Research Center Davis CA USADepartment of Nutrition Sciences University of Alabama Birmingham AL USADepartment of Nutrition Sciences University of Alabama Birmingham AL USADepartment of Nutrition Sciences University of Alabama Birmingham AL USAPharmacology Department Case Western Reserve University Cleveland OH USADepartment of Biochemistry, Microbiology and Immunology, and Ottawa Institute of Systems Biology University of Ottawa Ottawa ON CanadaUnited States Department of Agriculture‐Agricultural Research Service Western Human Nutrition Research Center Davis CA USAArkansas Children’s Nutrition Center Little Rock AR USAAbstract Very little is known about how metabolic health status, insulin resistance or metabolic challenges modulate the endocannabinoid (eCB) or polyunsaturated fatty acid (PUFA)‐derived oxylipin (OxL) lipid classes. To address these questions, plasma eCB and OxL concentrations were determined at rest, 10 and 20 min during an acute exercise bout (30 min total, ~45% of preintervention V̇O2peak, ~63 W), and following 20 min recovery in overnight‐fasted sedentary, obese, insulin‐resistant women under controlled diet conditions. We hypothesized that increased fitness and insulin sensitivity following a ~14‐week training and weight loss intervention would lead to significant changes in lipid signatures using an identical acute exercise protocol to preintervention. In the first 10 min of exercise, concentrations of a suite of OxL diols and hydroxyeicosatetraenoic acid (HETE) metabolites dropped significantly. There was no increase in 12,13‐DiHOME, previously reported to increase with exercise and proposed to activate muscle fatty acid uptake and tissue metabolism. Following weight loss intervention, exercise‐associated reductions were more pronounced for several linoleate and alpha‐linolenate metabolites including DiHOMEs, DiHODEs, KODEs, and EpODEs, and fasting concentrations of 9,10‐DiHODE, 12,13‐DiHODE, and 9,10‐DiHOME were reduced. These findings suggest that improved metabolic health modifies soluble epoxide hydrolase, cytochrome P450 epoxygenase (CYP), and lipoxygenase (LOX) systems. Acute exercise led to reductions for most eCB metabolites, with no evidence for concentration increases even at recovery. It is proposed that during submaximal aerobic exercise, nonoxidative fates of long‐chain saturated, monounsaturated, and PUFAs are attenuated in tissues that are important contributors to the blood OxL and eCB pools.https://doi.org/10.14814/phy2.14547oxylipidlipoxygenasesoluble epoxide hydrolasepolyunsaturated fatty acidPUFA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dmitry Grapov Oliver Fiehn Caitlin Campbell Carol J. Chandler Dustin J. Burnett Elaine C. Souza Gretchen A. Casazza Nancy L. Keim Gary R. Hunter Jose R. Fernandez W. Timothy Garvey Charles L. Hoppel Mary‐Ellen Harper John W. Newman Sean H. Adams |
spellingShingle |
Dmitry Grapov Oliver Fiehn Caitlin Campbell Carol J. Chandler Dustin J. Burnett Elaine C. Souza Gretchen A. Casazza Nancy L. Keim Gary R. Hunter Jose R. Fernandez W. Timothy Garvey Charles L. Hoppel Mary‐Ellen Harper John W. Newman Sean H. Adams Impact of a weight loss and fitness intervention on exercise‐associated plasma oxylipin patterns in obese, insulin‐resistant, sedentary women Physiological Reports oxylipid lipoxygenase soluble epoxide hydrolase polyunsaturated fatty acid PUFA |
author_facet |
Dmitry Grapov Oliver Fiehn Caitlin Campbell Carol J. Chandler Dustin J. Burnett Elaine C. Souza Gretchen A. Casazza Nancy L. Keim Gary R. Hunter Jose R. Fernandez W. Timothy Garvey Charles L. Hoppel Mary‐Ellen Harper John W. Newman Sean H. Adams |
author_sort |
Dmitry Grapov |
title |
Impact of a weight loss and fitness intervention on exercise‐associated plasma oxylipin patterns in obese, insulin‐resistant, sedentary women |
title_short |
Impact of a weight loss and fitness intervention on exercise‐associated plasma oxylipin patterns in obese, insulin‐resistant, sedentary women |
title_full |
Impact of a weight loss and fitness intervention on exercise‐associated plasma oxylipin patterns in obese, insulin‐resistant, sedentary women |
title_fullStr |
Impact of a weight loss and fitness intervention on exercise‐associated plasma oxylipin patterns in obese, insulin‐resistant, sedentary women |
title_full_unstemmed |
Impact of a weight loss and fitness intervention on exercise‐associated plasma oxylipin patterns in obese, insulin‐resistant, sedentary women |
title_sort |
impact of a weight loss and fitness intervention on exercise‐associated plasma oxylipin patterns in obese, insulin‐resistant, sedentary women |
publisher |
Wiley |
series |
Physiological Reports |
issn |
2051-817X |
publishDate |
2020-09-01 |
description |
Abstract Very little is known about how metabolic health status, insulin resistance or metabolic challenges modulate the endocannabinoid (eCB) or polyunsaturated fatty acid (PUFA)‐derived oxylipin (OxL) lipid classes. To address these questions, plasma eCB and OxL concentrations were determined at rest, 10 and 20 min during an acute exercise bout (30 min total, ~45% of preintervention V̇O2peak, ~63 W), and following 20 min recovery in overnight‐fasted sedentary, obese, insulin‐resistant women under controlled diet conditions. We hypothesized that increased fitness and insulin sensitivity following a ~14‐week training and weight loss intervention would lead to significant changes in lipid signatures using an identical acute exercise protocol to preintervention. In the first 10 min of exercise, concentrations of a suite of OxL diols and hydroxyeicosatetraenoic acid (HETE) metabolites dropped significantly. There was no increase in 12,13‐DiHOME, previously reported to increase with exercise and proposed to activate muscle fatty acid uptake and tissue metabolism. Following weight loss intervention, exercise‐associated reductions were more pronounced for several linoleate and alpha‐linolenate metabolites including DiHOMEs, DiHODEs, KODEs, and EpODEs, and fasting concentrations of 9,10‐DiHODE, 12,13‐DiHODE, and 9,10‐DiHOME were reduced. These findings suggest that improved metabolic health modifies soluble epoxide hydrolase, cytochrome P450 epoxygenase (CYP), and lipoxygenase (LOX) systems. Acute exercise led to reductions for most eCB metabolites, with no evidence for concentration increases even at recovery. It is proposed that during submaximal aerobic exercise, nonoxidative fates of long‐chain saturated, monounsaturated, and PUFAs are attenuated in tissues that are important contributors to the blood OxL and eCB pools. |
topic |
oxylipid lipoxygenase soluble epoxide hydrolase polyunsaturated fatty acid PUFA |
url |
https://doi.org/10.14814/phy2.14547 |
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