Yeast Nanometric Scale Oscillations Highlights Fibronectin Induced Changes in <i>C. albicans</i>
Yeast resistance to antifungal drugs is a major public health issue. Fungal adhesion onto the host mucosal surface is still a partially unknown phenomenon that is modulated by several actors among which fibronectin plays an important role. Targeting the yeast adhesion onto the mucosal surface could...
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doaj-f93eb1d78acd496c872ba7a55298a11a2020-11-25T01:41:39ZengMDPI AGFermentation2311-56372020-02-01612810.3390/fermentation6010028fermentation6010028Yeast Nanometric Scale Oscillations Highlights Fibronectin Induced Changes in <i>C. albicans</i>Anne-Céline Kohler0Leonardo Venturelli1Abhilash Kannan2Dominique Sanglard3Giovanni Dietler4Ronnie Willaert5Sandor Kasas6Laboratoire de Physique de la Matière Vivante, EPFL, 1015 Lausanne, SwitzerlandLaboratoire de Physique de la Matière Vivante, EPFL, 1015 Lausanne, SwitzerlandInstitute of Microbiology, Lausanne University Hospital, CH-1011 Lausanne, SwitzerlandInstitute of Microbiology, Lausanne University Hospital, CH-1011 Lausanne, SwitzerlandLaboratoire de Physique de la Matière Vivante, EPFL, 1015 Lausanne, SwitzerlandInternational Joint Research Group VUB-EPFL NanoBiotechnology & NanoMedicine (NANO), Vrije Universiteit Brussel,1050 Brussels, BelgiumLaboratoire de Physique de la Matière Vivante, EPFL, 1015 Lausanne, SwitzerlandYeast resistance to antifungal drugs is a major public health issue. Fungal adhesion onto the host mucosal surface is still a partially unknown phenomenon that is modulated by several actors among which fibronectin plays an important role. Targeting the yeast adhesion onto the mucosal surface could lead to potentially highly efficient treatments. In this work, we explored the effect of fibronectin on the nanomotion pattern of different <i>Candida albicans</i> strains by atomic force microscopy (AFM)-based nanomotion detection and correlated the cellular oscillations to the yeast adhesion onto epithelial cells. Preliminary results demonstrate that strongly adhering strains reduce their nanomotion activity upon fibronectin exposure whereas low adhering Candida remain unaffected. These results open novel avenues to explore cellular reactions upon exposure to stimulating agents and possibly to monitor in a rapid and simple manner adhesive properties of <i>C. albicans.</i>https://www.mdpi.com/2311-5637/6/1/28<i>candida</i> <i>albicans</i>adhesionfibronectinnanomotionatomic force microscope (afm) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anne-Céline Kohler Leonardo Venturelli Abhilash Kannan Dominique Sanglard Giovanni Dietler Ronnie Willaert Sandor Kasas |
spellingShingle |
Anne-Céline Kohler Leonardo Venturelli Abhilash Kannan Dominique Sanglard Giovanni Dietler Ronnie Willaert Sandor Kasas Yeast Nanometric Scale Oscillations Highlights Fibronectin Induced Changes in <i>C. albicans</i> Fermentation <i>candida</i> <i>albicans</i> adhesion fibronectin nanomotion atomic force microscope (afm) |
author_facet |
Anne-Céline Kohler Leonardo Venturelli Abhilash Kannan Dominique Sanglard Giovanni Dietler Ronnie Willaert Sandor Kasas |
author_sort |
Anne-Céline Kohler |
title |
Yeast Nanometric Scale Oscillations Highlights Fibronectin Induced Changes in <i>C. albicans</i> |
title_short |
Yeast Nanometric Scale Oscillations Highlights Fibronectin Induced Changes in <i>C. albicans</i> |
title_full |
Yeast Nanometric Scale Oscillations Highlights Fibronectin Induced Changes in <i>C. albicans</i> |
title_fullStr |
Yeast Nanometric Scale Oscillations Highlights Fibronectin Induced Changes in <i>C. albicans</i> |
title_full_unstemmed |
Yeast Nanometric Scale Oscillations Highlights Fibronectin Induced Changes in <i>C. albicans</i> |
title_sort |
yeast nanometric scale oscillations highlights fibronectin induced changes in <i>c. albicans</i> |
publisher |
MDPI AG |
series |
Fermentation |
issn |
2311-5637 |
publishDate |
2020-02-01 |
description |
Yeast resistance to antifungal drugs is a major public health issue. Fungal adhesion onto the host mucosal surface is still a partially unknown phenomenon that is modulated by several actors among which fibronectin plays an important role. Targeting the yeast adhesion onto the mucosal surface could lead to potentially highly efficient treatments. In this work, we explored the effect of fibronectin on the nanomotion pattern of different <i>Candida albicans</i> strains by atomic force microscopy (AFM)-based nanomotion detection and correlated the cellular oscillations to the yeast adhesion onto epithelial cells. Preliminary results demonstrate that strongly adhering strains reduce their nanomotion activity upon fibronectin exposure whereas low adhering Candida remain unaffected. These results open novel avenues to explore cellular reactions upon exposure to stimulating agents and possibly to monitor in a rapid and simple manner adhesive properties of <i>C. albicans.</i> |
topic |
<i>candida</i> <i>albicans</i> adhesion fibronectin nanomotion atomic force microscope (afm) |
url |
https://www.mdpi.com/2311-5637/6/1/28 |
work_keys_str_mv |
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1725040412861661184 |