Rapid Prototyping of Polymeric Nanopillars by 3D Direct Laser Writing for Controlling Cell Behavior
Abstract Mammalian cells have been widely shown to respond to nano- and microtopography that mimics the extracellular matrix. Synthetic nano- and micron-sized structures are therefore of great interest in the field of tissue engineering, where polymers are particularly attractive due to excellent bi...
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doaj-f93f07564d9d4e21b3902e5c871083172020-12-08T03:14:49ZengNature Publishing GroupScientific Reports2045-23222017-08-01711910.1038/s41598-017-09208-yRapid Prototyping of Polymeric Nanopillars by 3D Direct Laser Writing for Controlling Cell BehaviorNina Buch-Månson0Arnaud Spangenberg1Laura Piedad Chia Gomez2Jean-Pierre Malval3Olivier Soppera4Karen L. Martinez5Bionanotechnology and Nanomedicine Laboratory, Department of Chemistry and Nano-science Center, University of Copenhagen, Universitetsparken 5Institut de Science des Matériaux de Mulhouse (IS2M), CNRS - UMR 7361, Université de Haute Alsace, 15 rue Jean StarckyInstitut de Science des Matériaux de Mulhouse (IS2M), CNRS - UMR 7361, Université de Haute Alsace, 15 rue Jean StarckyInstitut de Science des Matériaux de Mulhouse (IS2M), CNRS - UMR 7361, Université de Haute Alsace, 15 rue Jean StarckyInstitut de Science des Matériaux de Mulhouse (IS2M), CNRS - UMR 7361, Université de Haute Alsace, 15 rue Jean StarckyBionanotechnology and Nanomedicine Laboratory, Department of Chemistry and Nano-science Center, University of Copenhagen, Universitetsparken 5Abstract Mammalian cells have been widely shown to respond to nano- and microtopography that mimics the extracellular matrix. Synthetic nano- and micron-sized structures are therefore of great interest in the field of tissue engineering, where polymers are particularly attractive due to excellent biocompatibility and versatile fabrication methods. Ordered arrays of polymeric pillars provide a controlled topographical environment to study and manipulate cells, but processing methods are typically either optimized for the nano- or microscale. Here, we demonstrate polymeric nanopillar (NP) fabrication using 3D direct laser writing (3D DLW), which offers a rapid prototyping across both size regimes. The NPs are interfaced with NIH3T3 cells and the effect of tuning geometrical parameters of the NP array is investigated. Cells are found to adhere on a wide range of geometries, but the interface depends on NP density and length. The Cell Interface with Nanostructure Arrays (CINA) model is successfully extended to predict the type of interface formed on different NP geometries, which is found to correlate with the efficiency of cell alignment along the NPs. The combination of the CINA model with the highly versatile 3D DLW fabrication thus holds the promise of improved design of polymeric NP arrays for controlling cell growth.https://doi.org/10.1038/s41598-017-09208-y |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nina Buch-Månson Arnaud Spangenberg Laura Piedad Chia Gomez Jean-Pierre Malval Olivier Soppera Karen L. Martinez |
spellingShingle |
Nina Buch-Månson Arnaud Spangenberg Laura Piedad Chia Gomez Jean-Pierre Malval Olivier Soppera Karen L. Martinez Rapid Prototyping of Polymeric Nanopillars by 3D Direct Laser Writing for Controlling Cell Behavior Scientific Reports |
author_facet |
Nina Buch-Månson Arnaud Spangenberg Laura Piedad Chia Gomez Jean-Pierre Malval Olivier Soppera Karen L. Martinez |
author_sort |
Nina Buch-Månson |
title |
Rapid Prototyping of Polymeric Nanopillars by 3D Direct Laser Writing for Controlling Cell Behavior |
title_short |
Rapid Prototyping of Polymeric Nanopillars by 3D Direct Laser Writing for Controlling Cell Behavior |
title_full |
Rapid Prototyping of Polymeric Nanopillars by 3D Direct Laser Writing for Controlling Cell Behavior |
title_fullStr |
Rapid Prototyping of Polymeric Nanopillars by 3D Direct Laser Writing for Controlling Cell Behavior |
title_full_unstemmed |
Rapid Prototyping of Polymeric Nanopillars by 3D Direct Laser Writing for Controlling Cell Behavior |
title_sort |
rapid prototyping of polymeric nanopillars by 3d direct laser writing for controlling cell behavior |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-08-01 |
description |
Abstract Mammalian cells have been widely shown to respond to nano- and microtopography that mimics the extracellular matrix. Synthetic nano- and micron-sized structures are therefore of great interest in the field of tissue engineering, where polymers are particularly attractive due to excellent biocompatibility and versatile fabrication methods. Ordered arrays of polymeric pillars provide a controlled topographical environment to study and manipulate cells, but processing methods are typically either optimized for the nano- or microscale. Here, we demonstrate polymeric nanopillar (NP) fabrication using 3D direct laser writing (3D DLW), which offers a rapid prototyping across both size regimes. The NPs are interfaced with NIH3T3 cells and the effect of tuning geometrical parameters of the NP array is investigated. Cells are found to adhere on a wide range of geometries, but the interface depends on NP density and length. The Cell Interface with Nanostructure Arrays (CINA) model is successfully extended to predict the type of interface formed on different NP geometries, which is found to correlate with the efficiency of cell alignment along the NPs. The combination of the CINA model with the highly versatile 3D DLW fabrication thus holds the promise of improved design of polymeric NP arrays for controlling cell growth. |
url |
https://doi.org/10.1038/s41598-017-09208-y |
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