Cryo-Electron Microscopy Structure of the Macrobrachium rosenbergii Nodavirus Capsid at 7 Angstroms Resolution
Abstract White tail disease in the giant freshwater prawn Macrobrachium rosenbergii causes significant economic losses in shrimp farms and hatcheries and poses a threat to food-security in many developing countries. Outbreaks of Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of whit...
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doaj-f953ba71eea14ac0baeed379a37177122020-12-08T03:02:21ZengNature Publishing GroupScientific Reports2045-23222017-05-01711810.1038/s41598-017-02292-0Cryo-Electron Microscopy Structure of the Macrobrachium rosenbergii Nodavirus Capsid at 7 Angstroms ResolutionKok Lian Ho0Chare Li Kueh1Poay Ling Beh2Wen Siang Tan3David Bhella4Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra MalaysiaDepartment of Microbiology, Faculty of Biotechnology and Biomolecular SciencesDepartment of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra MalaysiaDepartment of Microbiology, Faculty of Biotechnology and Biomolecular SciencesMRC-University of Glasgow Centre for Virus Research, Sir Michael Stoker Building, Garscube CampusAbstract White tail disease in the giant freshwater prawn Macrobrachium rosenbergii causes significant economic losses in shrimp farms and hatcheries and poses a threat to food-security in many developing countries. Outbreaks of Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white tail disease (WTD) are associated with up to 100% mortality rates. There are no interventions available to treat or prevent MrNV disease however. Here we show the structure of MrNV virus-like particles (VLPs) produced by recombinant expression of the capsid protein, using cryogenic electron microscopy. Our data show that MrNV VLPs package nucleic acids in a manner reminiscent of other known nodavirus structures. The structure of the capsid however shows striking differences from insect and fish infecting nodaviruses, which have been shown to assemble trimer-clustered T = 3 icosahedral virus particles. MrNV particles have pronounced dimeric blade-shaped spikes extending up to 6 nm from the outer surface of the capsid shell. Our structural analysis supports the assertion that MrNV may belong to a new genus of the Nodaviridae. Moreover, our study provides the first structural view of an important pathogen affecting aquaculture industries across the world.https://doi.org/10.1038/s41598-017-02292-0 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kok Lian Ho Chare Li Kueh Poay Ling Beh Wen Siang Tan David Bhella |
spellingShingle |
Kok Lian Ho Chare Li Kueh Poay Ling Beh Wen Siang Tan David Bhella Cryo-Electron Microscopy Structure of the Macrobrachium rosenbergii Nodavirus Capsid at 7 Angstroms Resolution Scientific Reports |
author_facet |
Kok Lian Ho Chare Li Kueh Poay Ling Beh Wen Siang Tan David Bhella |
author_sort |
Kok Lian Ho |
title |
Cryo-Electron Microscopy Structure of the Macrobrachium rosenbergii Nodavirus Capsid at 7 Angstroms Resolution |
title_short |
Cryo-Electron Microscopy Structure of the Macrobrachium rosenbergii Nodavirus Capsid at 7 Angstroms Resolution |
title_full |
Cryo-Electron Microscopy Structure of the Macrobrachium rosenbergii Nodavirus Capsid at 7 Angstroms Resolution |
title_fullStr |
Cryo-Electron Microscopy Structure of the Macrobrachium rosenbergii Nodavirus Capsid at 7 Angstroms Resolution |
title_full_unstemmed |
Cryo-Electron Microscopy Structure of the Macrobrachium rosenbergii Nodavirus Capsid at 7 Angstroms Resolution |
title_sort |
cryo-electron microscopy structure of the macrobrachium rosenbergii nodavirus capsid at 7 angstroms resolution |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-05-01 |
description |
Abstract White tail disease in the giant freshwater prawn Macrobrachium rosenbergii causes significant economic losses in shrimp farms and hatcheries and poses a threat to food-security in many developing countries. Outbreaks of Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white tail disease (WTD) are associated with up to 100% mortality rates. There are no interventions available to treat or prevent MrNV disease however. Here we show the structure of MrNV virus-like particles (VLPs) produced by recombinant expression of the capsid protein, using cryogenic electron microscopy. Our data show that MrNV VLPs package nucleic acids in a manner reminiscent of other known nodavirus structures. The structure of the capsid however shows striking differences from insect and fish infecting nodaviruses, which have been shown to assemble trimer-clustered T = 3 icosahedral virus particles. MrNV particles have pronounced dimeric blade-shaped spikes extending up to 6 nm from the outer surface of the capsid shell. Our structural analysis supports the assertion that MrNV may belong to a new genus of the Nodaviridae. Moreover, our study provides the first structural view of an important pathogen affecting aquaculture industries across the world. |
url |
https://doi.org/10.1038/s41598-017-02292-0 |
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