Characterization of Burn Eschar Pericytes
Pericytes are cells that reside adjacent to microvasculature and regulate vascular function. Pericytes gained great interest in the field of wound healing and regenerative medicine due to their multipotential fate and ability to enhance angiogenesis. In burn wounds, scarring and scar contractures ar...
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doaj-f954ecf1abed4c87beab09de6808a0222020-11-25T02:09:20ZengMDPI AGJournal of Clinical Medicine2077-03832020-02-019260610.3390/jcm9020606jcm9020606Characterization of Burn Eschar PericytesAlexander Evdokiou0Onur Kanisicak1Stephanie Gierek2Amanda Barry3Malina J. Ivey4Xiang Zhang5Richard J. Bodnar6Latha Satish7Shriners Hospitals for Children, Research Department, Cincinnati, OH 45229, USADepartment of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45267-0529, USAShriners Hospitals for Children, Research Department, Cincinnati, OH 45229, USAShriners Hospitals for Children, Research Department, Cincinnati, OH 45229, USADepartment of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, OH 45267-0529, USAGenomics, Epigenomics and Sequencing Core, University of Cincinnati, Cincinnati, OH 45267, USAVeterans Affairs Medical Center, University Dr. C, Pittsburgh, PA 15240, USAShriners Hospitals for Children, Research Department, Cincinnati, OH 45229, USAPericytes are cells that reside adjacent to microvasculature and regulate vascular function. Pericytes gained great interest in the field of wound healing and regenerative medicine due to their multipotential fate and ability to enhance angiogenesis. In burn wounds, scarring and scar contractures are the major pathologic feature and cause loss of mobility. The present study investigated the influence of burn wound environment on pericytes during wound healing. Pericytes isolated from normal skin and tangentially excised burn eschar tissues were analyzed for differences in gene and protein expression using RNA-seq., immunocytochemistry, and ELISA analyses. RNA-seq identified 443 differentially expressed genes between normal- and burn eschar-derived pericytes. Whereas, comparing normal skin pericytes to normal skin fibroblasts identified 1021 distinct genes and comparing burn eschar pericytes to normal skin fibroblasts identified 2449 differential genes. Altogether, forkhead box E1 (FOXE1), a transcription factor, was identified as a unique marker for skin pericytes. Interestingly, FOXE1 levels were significantly elevated in burn eschar pericytes compared to normal. Additionally, burn wound pericytes showed increased expression of profibrotic genes periostin, fibronectin, and endosialin and a gain in contractile function, suggesting a contribution to scarring and fibrosis. Our findings suggest that the burn wound environment promotes pericytes to differentiate into a myofibroblast-like phenotype promoting scar formation and fibrosis.https://www.mdpi.com/2077-0383/9/2/606pericyteperiostinfoxe1il-6il-8myd88tgf-βendosialinburns and stem cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alexander Evdokiou Onur Kanisicak Stephanie Gierek Amanda Barry Malina J. Ivey Xiang Zhang Richard J. Bodnar Latha Satish |
spellingShingle |
Alexander Evdokiou Onur Kanisicak Stephanie Gierek Amanda Barry Malina J. Ivey Xiang Zhang Richard J. Bodnar Latha Satish Characterization of Burn Eschar Pericytes Journal of Clinical Medicine pericyte periostin foxe1 il-6 il-8 myd88 tgf-β endosialin burns and stem cells |
author_facet |
Alexander Evdokiou Onur Kanisicak Stephanie Gierek Amanda Barry Malina J. Ivey Xiang Zhang Richard J. Bodnar Latha Satish |
author_sort |
Alexander Evdokiou |
title |
Characterization of Burn Eschar Pericytes |
title_short |
Characterization of Burn Eschar Pericytes |
title_full |
Characterization of Burn Eschar Pericytes |
title_fullStr |
Characterization of Burn Eschar Pericytes |
title_full_unstemmed |
Characterization of Burn Eschar Pericytes |
title_sort |
characterization of burn eschar pericytes |
publisher |
MDPI AG |
series |
Journal of Clinical Medicine |
issn |
2077-0383 |
publishDate |
2020-02-01 |
description |
Pericytes are cells that reside adjacent to microvasculature and regulate vascular function. Pericytes gained great interest in the field of wound healing and regenerative medicine due to their multipotential fate and ability to enhance angiogenesis. In burn wounds, scarring and scar contractures are the major pathologic feature and cause loss of mobility. The present study investigated the influence of burn wound environment on pericytes during wound healing. Pericytes isolated from normal skin and tangentially excised burn eschar tissues were analyzed for differences in gene and protein expression using RNA-seq., immunocytochemistry, and ELISA analyses. RNA-seq identified 443 differentially expressed genes between normal- and burn eschar-derived pericytes. Whereas, comparing normal skin pericytes to normal skin fibroblasts identified 1021 distinct genes and comparing burn eschar pericytes to normal skin fibroblasts identified 2449 differential genes. Altogether, forkhead box E1 (FOXE1), a transcription factor, was identified as a unique marker for skin pericytes. Interestingly, FOXE1 levels were significantly elevated in burn eschar pericytes compared to normal. Additionally, burn wound pericytes showed increased expression of profibrotic genes periostin, fibronectin, and endosialin and a gain in contractile function, suggesting a contribution to scarring and fibrosis. Our findings suggest that the burn wound environment promotes pericytes to differentiate into a myofibroblast-like phenotype promoting scar formation and fibrosis. |
topic |
pericyte periostin foxe1 il-6 il-8 myd88 tgf-β endosialin burns and stem cells |
url |
https://www.mdpi.com/2077-0383/9/2/606 |
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