Novel role of NOX in supporting aerobic glycolysis in cancer cells with mitochondrial dysfunction and as a potential target for cancer therapy.
Elevated aerobic glycolysis in cancer cells (the Warburg effect) may be attributed to respiration injury or mitochondrial dysfunction, but the underlying mechanisms and therapeutic significance remain elusive. Here we report that induction of mitochondrial respiratory defect by tetracycline-controll...
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2012-01-01
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doaj-f958bb68bef44488b198923a9af471a92021-07-02T13:43:54ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852012-01-01105e100132610.1371/journal.pbio.1001326Novel role of NOX in supporting aerobic glycolysis in cancer cells with mitochondrial dysfunction and as a potential target for cancer therapy.Weiqin LuYumin HuGang ChenZhao ChenHui ZhangFeng WangLi FengHelene PelicanoHua WangMichael J KeatingJinsong LiuWallace McKeehanHuamin WangYongde LuoPeng HuangElevated aerobic glycolysis in cancer cells (the Warburg effect) may be attributed to respiration injury or mitochondrial dysfunction, but the underlying mechanisms and therapeutic significance remain elusive. Here we report that induction of mitochondrial respiratory defect by tetracycline-controlled expression of a dominant negative form of DNA polymerase γ causes a metabolic shift from oxidative phosphorylation to glycolysis and increases ROS generation. We show that upregulation of NOX is critical to support the elevated glycolysis by providing additional NAD+. The upregulation of NOX is also consistently observed in cancer cells with compromised mitochondria due to the activation of oncogenic Ras or loss of p53, and in primary pancreatic cancer tissues. Suppression of NOX by chemical inhibition or genetic knockdown of gene expression selectively impacts cancer cells with mitochondrial dysfunction, leading to a decrease in cellular glycolysis, a loss of cell viability, and inhibition of cancer growth in vivo. Our study reveals a previously unrecognized function of NOX in cancer metabolism and suggests that NOX is a potential novel target for cancer treatment.http://europepmc.org/articles/PMC3348157?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Weiqin Lu Yumin Hu Gang Chen Zhao Chen Hui Zhang Feng Wang Li Feng Helene Pelicano Hua Wang Michael J Keating Jinsong Liu Wallace McKeehan Huamin Wang Yongde Luo Peng Huang |
spellingShingle |
Weiqin Lu Yumin Hu Gang Chen Zhao Chen Hui Zhang Feng Wang Li Feng Helene Pelicano Hua Wang Michael J Keating Jinsong Liu Wallace McKeehan Huamin Wang Yongde Luo Peng Huang Novel role of NOX in supporting aerobic glycolysis in cancer cells with mitochondrial dysfunction and as a potential target for cancer therapy. PLoS Biology |
author_facet |
Weiqin Lu Yumin Hu Gang Chen Zhao Chen Hui Zhang Feng Wang Li Feng Helene Pelicano Hua Wang Michael J Keating Jinsong Liu Wallace McKeehan Huamin Wang Yongde Luo Peng Huang |
author_sort |
Weiqin Lu |
title |
Novel role of NOX in supporting aerobic glycolysis in cancer cells with mitochondrial dysfunction and as a potential target for cancer therapy. |
title_short |
Novel role of NOX in supporting aerobic glycolysis in cancer cells with mitochondrial dysfunction and as a potential target for cancer therapy. |
title_full |
Novel role of NOX in supporting aerobic glycolysis in cancer cells with mitochondrial dysfunction and as a potential target for cancer therapy. |
title_fullStr |
Novel role of NOX in supporting aerobic glycolysis in cancer cells with mitochondrial dysfunction and as a potential target for cancer therapy. |
title_full_unstemmed |
Novel role of NOX in supporting aerobic glycolysis in cancer cells with mitochondrial dysfunction and as a potential target for cancer therapy. |
title_sort |
novel role of nox in supporting aerobic glycolysis in cancer cells with mitochondrial dysfunction and as a potential target for cancer therapy. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Biology |
issn |
1544-9173 1545-7885 |
publishDate |
2012-01-01 |
description |
Elevated aerobic glycolysis in cancer cells (the Warburg effect) may be attributed to respiration injury or mitochondrial dysfunction, but the underlying mechanisms and therapeutic significance remain elusive. Here we report that induction of mitochondrial respiratory defect by tetracycline-controlled expression of a dominant negative form of DNA polymerase γ causes a metabolic shift from oxidative phosphorylation to glycolysis and increases ROS generation. We show that upregulation of NOX is critical to support the elevated glycolysis by providing additional NAD+. The upregulation of NOX is also consistently observed in cancer cells with compromised mitochondria due to the activation of oncogenic Ras or loss of p53, and in primary pancreatic cancer tissues. Suppression of NOX by chemical inhibition or genetic knockdown of gene expression selectively impacts cancer cells with mitochondrial dysfunction, leading to a decrease in cellular glycolysis, a loss of cell viability, and inhibition of cancer growth in vivo. Our study reveals a previously unrecognized function of NOX in cancer metabolism and suggests that NOX is a potential novel target for cancer treatment. |
url |
http://europepmc.org/articles/PMC3348157?pdf=render |
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