Is day 7 culture necessary for in vitro fertilization of cryopreserved/warmed human oocytes?
Abstract Background Human embryos are usually cultured to blastocyst stage by Day 5 or 6 after insemination. However, some embryos grow slowly and reach blastocyst stage at Day 7. Acceptable live birth rates have been reported after transfer of Day 7 blastocysts resulted from fresh oocyte in vitro f...
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doaj-f9605053e7b249ca8edb4aa40d5b310f2021-01-17T12:10:27ZengBMCReproductive Biology and Endocrinology1477-78272020-01-011811410.1186/s12958-020-0565-9Is day 7 culture necessary for in vitro fertilization of cryopreserved/warmed human oocytes?Xiangli Niu0Cassie T. Wang1Richard Li2Ghassan Haddad3Weihua Wang4Research Center for Reproductive Medicine, Reproductive Hospital of Guangxi Zhuang Autonomous RegionPrelude-Houston Fertility Laboratory, Houston Fertility InstitutePrelude-Houston Fertility Laboratory, Houston Fertility InstituteHouston Fertility InstitutePrelude-Houston Fertility Laboratory, Houston Fertility InstituteAbstract Background Human embryos are usually cultured to blastocyst stage by Day 5 or 6 after insemination. However, some embryos grow slowly and reach blastocyst stage at Day 7. Acceptable live birth rates have been reported after transfer of Day 7 blastocysts resulted from fresh oocyte in vitro fertilization (IVF). It is unknown whether an extended embryo culture to Day 7 is necessary for cryopreserved oocyte IVF to obtain more transferrable blastocysts. Methods In this study, 455 oocytes from 57 cycles were warmed, inseminated, and the resulting embryos were cultured by Day 7 to examine blastocyst development after extended culture. Fifty one blastocysts from 16 cycles were biopsied to examine embryo aneuploidies. Results It was found that 35.1% of the cycles had Day 7 blastocysts, and 3.5% of the cycles had only Day 7 blastocysts. Day 7 blastocysts accounted for 15.6% of total blastocysts. The proportion of top quality of blastocysts was lower at Day 7 than at Day 5 or 6. However, no differences were observed on aneuploid blastocyst rates among Days 5, 6 and 7. Similar clinical pregnancy, ongoing pregnancy and embryo implantation rates were obtained after Day 7 blastocyst transfer as compared with Day 5 or 6 blastocyst transfer. Conclusion These results indicate that embryos from oocyte warming cycles should be cultured to Day 7 if they do not reach to blastocyst stage by Day 6 so that number of usable blastocysts can be increased.https://doi.org/10.1186/s12958-020-0565-9OocytesWarmingBlastocystsDay 7 cultureAneuploidy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiangli Niu Cassie T. Wang Richard Li Ghassan Haddad Weihua Wang |
spellingShingle |
Xiangli Niu Cassie T. Wang Richard Li Ghassan Haddad Weihua Wang Is day 7 culture necessary for in vitro fertilization of cryopreserved/warmed human oocytes? Reproductive Biology and Endocrinology Oocytes Warming Blastocysts Day 7 culture Aneuploidy |
author_facet |
Xiangli Niu Cassie T. Wang Richard Li Ghassan Haddad Weihua Wang |
author_sort |
Xiangli Niu |
title |
Is day 7 culture necessary for in vitro fertilization of cryopreserved/warmed human oocytes? |
title_short |
Is day 7 culture necessary for in vitro fertilization of cryopreserved/warmed human oocytes? |
title_full |
Is day 7 culture necessary for in vitro fertilization of cryopreserved/warmed human oocytes? |
title_fullStr |
Is day 7 culture necessary for in vitro fertilization of cryopreserved/warmed human oocytes? |
title_full_unstemmed |
Is day 7 culture necessary for in vitro fertilization of cryopreserved/warmed human oocytes? |
title_sort |
is day 7 culture necessary for in vitro fertilization of cryopreserved/warmed human oocytes? |
publisher |
BMC |
series |
Reproductive Biology and Endocrinology |
issn |
1477-7827 |
publishDate |
2020-01-01 |
description |
Abstract Background Human embryos are usually cultured to blastocyst stage by Day 5 or 6 after insemination. However, some embryos grow slowly and reach blastocyst stage at Day 7. Acceptable live birth rates have been reported after transfer of Day 7 blastocysts resulted from fresh oocyte in vitro fertilization (IVF). It is unknown whether an extended embryo culture to Day 7 is necessary for cryopreserved oocyte IVF to obtain more transferrable blastocysts. Methods In this study, 455 oocytes from 57 cycles were warmed, inseminated, and the resulting embryos were cultured by Day 7 to examine blastocyst development after extended culture. Fifty one blastocysts from 16 cycles were biopsied to examine embryo aneuploidies. Results It was found that 35.1% of the cycles had Day 7 blastocysts, and 3.5% of the cycles had only Day 7 blastocysts. Day 7 blastocysts accounted for 15.6% of total blastocysts. The proportion of top quality of blastocysts was lower at Day 7 than at Day 5 or 6. However, no differences were observed on aneuploid blastocyst rates among Days 5, 6 and 7. Similar clinical pregnancy, ongoing pregnancy and embryo implantation rates were obtained after Day 7 blastocyst transfer as compared with Day 5 or 6 blastocyst transfer. Conclusion These results indicate that embryos from oocyte warming cycles should be cultured to Day 7 if they do not reach to blastocyst stage by Day 6 so that number of usable blastocysts can be increased. |
topic |
Oocytes Warming Blastocysts Day 7 culture Aneuploidy |
url |
https://doi.org/10.1186/s12958-020-0565-9 |
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