Plasma Lysyl-tRNA Synthetase 1 (KARS1) as a Novel Diagnostic and Monitoring Biomarker for Colorectal Cancer
Colorectal cancer (CRC) is one of the leading causes of world cancer deaths. To improve the survival rate of CRC, diagnosis and post-operative monitoring is necessary. Currently, biomarkers are used for CRC diagnosis and prognosis. However, these biomarkers have limitations of specificity and sensit...
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doaj-f966a3ce6d9c47a3b53ae77a581999032020-11-25T02:03:23ZengMDPI AGJournal of Clinical Medicine2077-03832020-02-019253310.3390/jcm9020533jcm9020533Plasma Lysyl-tRNA Synthetase 1 (KARS1) as a Novel Diagnostic and Monitoring Biomarker for Colorectal CancerJi Hun Suh0Min Chul Park1Peter C. Goughnour2Byung Soh Min3Sang Bum Kim4Woo Yong Lee5Yong Beom Cho6Jae Hee Cheon7Kang Young Lee8Do-Hyun Nam9Sunghoon Kim10Medicinal Bioconvergence Research Center, College of Pharmacy, Seoul National University, Seoul 08826, KoreaMedicinal Bioconvergence Research Center, College of Pharmacy, Seoul National University, Seoul 08826, KoreaMedicinal Bioconvergence Research Center, College of Pharmacy, Seoul National University, Seoul 08826, KoreaSeoul Republic of Korea Department of Surgery, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, KoreaMedicinal Bioconvergence Research Center, College of Pharmacy, Seoul National University, Seoul 08826, KoreaDepartment of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, KoreaDepartment of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, KoreaDepartment of Internal Medicine and Institute of Gastroenterology, Yonsei University College of Medicine, Seoul 03722, KoreaSeoul Republic of Korea Department of Surgery, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment of Health Sciences and Technology, Samsung Advanced Institute for Health Sciences and Technology, Sungkyunkwan University, Seoul 06351, KoreaMedicinal Bioconvergence Research Center, College of Pharmacy, Seoul National University, Seoul 08826, KoreaColorectal cancer (CRC) is one of the leading causes of world cancer deaths. To improve the survival rate of CRC, diagnosis and post-operative monitoring is necessary. Currently, biomarkers are used for CRC diagnosis and prognosis. However, these biomarkers have limitations of specificity and sensitivity. Levels of plasma lysyl-tRNA synthetase (KARS1), which was reported to be secreted from colon cancer cells by stimuli, along with other secreted aminoacyl-tRNA synthetases (ARSs), were analyzed in CRC and compared with the currently used biomarkers. The KARS1 levels of CRC patients (<i>n</i> = 164) plasma were shown to be higher than those of healthy volunteers (<i>n</i> = 32). The diagnostic values of plasma KARS1 were also evaluated by receiving operating characteristic (ROC) curve. Compared with other biomarkers and ARSs, KARS1 showed the best diagnostic value for CRC. The cancer specificity and burden correlation of plasma KARS1 level were validated using azoxymethane (AOM)/dextran sodium sulfate (DSS) model, and paired pre- and post-surgery CRC patient plasma. In the AOM/DSS model, the plasma level of KARS1 showed high correlation with number of polyps, but not for inflammation. Using paired pre- and post-surgery CRC plasma samples (<i>n</i> = 60), the plasma level of KARS1 was significantly decreased in post-surgery samples. Based on these evidence, KARS1, a surrogate biomarker reflecting CRC burden, can be used as a novel diagnostic and post-operative monitoring biomarker for CRC.https://www.mdpi.com/2077-0383/9/2/533lysyl-trna synthetasecolorectal cancerserologic biomarkerdiagnosismonitoringnoninvasive method |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ji Hun Suh Min Chul Park Peter C. Goughnour Byung Soh Min Sang Bum Kim Woo Yong Lee Yong Beom Cho Jae Hee Cheon Kang Young Lee Do-Hyun Nam Sunghoon Kim |
spellingShingle |
Ji Hun Suh Min Chul Park Peter C. Goughnour Byung Soh Min Sang Bum Kim Woo Yong Lee Yong Beom Cho Jae Hee Cheon Kang Young Lee Do-Hyun Nam Sunghoon Kim Plasma Lysyl-tRNA Synthetase 1 (KARS1) as a Novel Diagnostic and Monitoring Biomarker for Colorectal Cancer Journal of Clinical Medicine lysyl-trna synthetase colorectal cancer serologic biomarker diagnosis monitoring noninvasive method |
author_facet |
Ji Hun Suh Min Chul Park Peter C. Goughnour Byung Soh Min Sang Bum Kim Woo Yong Lee Yong Beom Cho Jae Hee Cheon Kang Young Lee Do-Hyun Nam Sunghoon Kim |
author_sort |
Ji Hun Suh |
title |
Plasma Lysyl-tRNA Synthetase 1 (KARS1) as a Novel Diagnostic and Monitoring Biomarker for Colorectal Cancer |
title_short |
Plasma Lysyl-tRNA Synthetase 1 (KARS1) as a Novel Diagnostic and Monitoring Biomarker for Colorectal Cancer |
title_full |
Plasma Lysyl-tRNA Synthetase 1 (KARS1) as a Novel Diagnostic and Monitoring Biomarker for Colorectal Cancer |
title_fullStr |
Plasma Lysyl-tRNA Synthetase 1 (KARS1) as a Novel Diagnostic and Monitoring Biomarker for Colorectal Cancer |
title_full_unstemmed |
Plasma Lysyl-tRNA Synthetase 1 (KARS1) as a Novel Diagnostic and Monitoring Biomarker for Colorectal Cancer |
title_sort |
plasma lysyl-trna synthetase 1 (kars1) as a novel diagnostic and monitoring biomarker for colorectal cancer |
publisher |
MDPI AG |
series |
Journal of Clinical Medicine |
issn |
2077-0383 |
publishDate |
2020-02-01 |
description |
Colorectal cancer (CRC) is one of the leading causes of world cancer deaths. To improve the survival rate of CRC, diagnosis and post-operative monitoring is necessary. Currently, biomarkers are used for CRC diagnosis and prognosis. However, these biomarkers have limitations of specificity and sensitivity. Levels of plasma lysyl-tRNA synthetase (KARS1), which was reported to be secreted from colon cancer cells by stimuli, along with other secreted aminoacyl-tRNA synthetases (ARSs), were analyzed in CRC and compared with the currently used biomarkers. The KARS1 levels of CRC patients (<i>n</i> = 164) plasma were shown to be higher than those of healthy volunteers (<i>n</i> = 32). The diagnostic values of plasma KARS1 were also evaluated by receiving operating characteristic (ROC) curve. Compared with other biomarkers and ARSs, KARS1 showed the best diagnostic value for CRC. The cancer specificity and burden correlation of plasma KARS1 level were validated using azoxymethane (AOM)/dextran sodium sulfate (DSS) model, and paired pre- and post-surgery CRC patient plasma. In the AOM/DSS model, the plasma level of KARS1 showed high correlation with number of polyps, but not for inflammation. Using paired pre- and post-surgery CRC plasma samples (<i>n</i> = 60), the plasma level of KARS1 was significantly decreased in post-surgery samples. Based on these evidence, KARS1, a surrogate biomarker reflecting CRC burden, can be used as a novel diagnostic and post-operative monitoring biomarker for CRC. |
topic |
lysyl-trna synthetase colorectal cancer serologic biomarker diagnosis monitoring noninvasive method |
url |
https://www.mdpi.com/2077-0383/9/2/533 |
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