Blood biomarkers of common toad Rhinella arenarum following chlorpyrifos dermal exposure
Chlorpyrifos (CPF) is a broad spectrum pesticide commonly used for insect control, has great affinity for lipids and is thus a potential for bioaccumulation in aquatic organisms. The aim of this study was to evaluate the toxicity of CPF using the common toad Rhinella arenarum via dermal uptake in pl...
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doaj-f988e6f9766f471baa529b7d154359ee2021-09-05T21:02:06ZengSciendoInterdisciplinary Toxicology1337-95692018-08-0111214815410.2478/intox-2018-0011intox-2018-0011Blood biomarkers of common toad Rhinella arenarum following chlorpyrifos dermal exposureLajmanovich Rafael C.0Peltzer Paola M.1Attademo Andrés M.2Colussi Carlina L.3Martinuzzi Candela S.4Laboratorio de Ecotoxicología, Facultad de Bioquímica y Ciencias Biológicas, FBCB-UNL, Santa Fe, ArgentinaLaboratorio de Ecotoxicología, Facultad de Bioquímica y Ciencias Biológicas, FBCB-UNL, Santa Fe, ArgentinaLaboratorio de Ecotoxicología, Facultad de Bioquímica y Ciencias Biológicas, FBCB-UNL, Santa Fe, ArgentinaLaboratorio de Ecotoxicología, Facultad de Bioquímica y Ciencias Biológicas, FBCB-UNL, Santa Fe, ArgentinaLaboratorio de Ecotoxicología, Facultad de Bioquímica y Ciencias Biológicas, FBCB-UNL, Santa Fe, ArgentinaChlorpyrifos (CPF) is a broad spectrum pesticide commonly used for insect control, has great affinity for lipids and is thus a potential for bioaccumulation in aquatic organisms. The aim of this study was to evaluate the toxicity of CPF using the common toad Rhinella arenarum via dermal uptake in plastic bucket to simulate their natural exposition in ponds. R. arenarum toads were exposed individually to solutions containing a nominal concentration of a commercial formulation of CPF insecticide (5 and 10 mg/L). Different enzyme biomarkers (BChE: butyrylcholinesterase, CbE: carboxylesterase, and CAT: catalase) were measured in blood tissue after exposition. The capacity of pyridine-2-aldoxime methochloride (2-PAM) to reverse OP-inhibited plasma BChE and the ratio of heterophils and lymphocytes (H/L) as hematological indicators of stress were also determined. The normal values of plasma B-sterases (BChE and CbE) were highly inhibited (until ≈ 70%) in toads 48 h after exposure to CPF. The results indicate that 2-PAM produced BChE reactivation as well. The activity of CAT was also inducted for dermal exposure at more than double of that in the control toads (CPF; 5 mg/L). H/L ratios did not reveal a significantly increased stress. The study suggests that CPF via dermal uptake induced neurotoxicity and oxidative stress in the common toad R. areanum. Thus, some blood biomarkers employed in our study (i.e. BChE, CbE, 2-PAM, and CAT) might be used as predictors in health and ecological risk assessment of amphibian populations exposed to CPF.https://doi.org/10.2478/intox-2018-0011rhinella arenarumbloodchlorpyrifos dermal exposureb-esterasescatalase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lajmanovich Rafael C. Peltzer Paola M. Attademo Andrés M. Colussi Carlina L. Martinuzzi Candela S. |
spellingShingle |
Lajmanovich Rafael C. Peltzer Paola M. Attademo Andrés M. Colussi Carlina L. Martinuzzi Candela S. Blood biomarkers of common toad Rhinella arenarum following chlorpyrifos dermal exposure Interdisciplinary Toxicology rhinella arenarum blood chlorpyrifos dermal exposure b-esterases catalase |
author_facet |
Lajmanovich Rafael C. Peltzer Paola M. Attademo Andrés M. Colussi Carlina L. Martinuzzi Candela S. |
author_sort |
Lajmanovich Rafael C. |
title |
Blood biomarkers of common toad Rhinella arenarum following chlorpyrifos dermal exposure |
title_short |
Blood biomarkers of common toad Rhinella arenarum following chlorpyrifos dermal exposure |
title_full |
Blood biomarkers of common toad Rhinella arenarum following chlorpyrifos dermal exposure |
title_fullStr |
Blood biomarkers of common toad Rhinella arenarum following chlorpyrifos dermal exposure |
title_full_unstemmed |
Blood biomarkers of common toad Rhinella arenarum following chlorpyrifos dermal exposure |
title_sort |
blood biomarkers of common toad rhinella arenarum following chlorpyrifos dermal exposure |
publisher |
Sciendo |
series |
Interdisciplinary Toxicology |
issn |
1337-9569 |
publishDate |
2018-08-01 |
description |
Chlorpyrifos (CPF) is a broad spectrum pesticide commonly used for insect control, has great affinity for lipids and is thus a potential for bioaccumulation in aquatic organisms. The aim of this study was to evaluate the toxicity of CPF using the common toad Rhinella arenarum via dermal uptake in plastic bucket to simulate their natural exposition in ponds. R. arenarum toads were exposed individually to solutions containing a nominal concentration of a commercial formulation of CPF insecticide (5 and 10 mg/L). Different enzyme biomarkers (BChE: butyrylcholinesterase, CbE: carboxylesterase, and CAT: catalase) were measured in blood tissue after exposition. The capacity of pyridine-2-aldoxime methochloride (2-PAM) to reverse OP-inhibited plasma BChE and the ratio of heterophils and lymphocytes (H/L) as hematological indicators of stress were also determined. The normal values of plasma B-sterases (BChE and CbE) were highly inhibited (until ≈ 70%) in toads 48 h after exposure to CPF. The results indicate that 2-PAM produced BChE reactivation as well. The activity of CAT was also inducted for dermal exposure at more than double of that in the control toads (CPF; 5 mg/L). H/L ratios did not reveal a significantly increased stress. The study suggests that CPF via dermal uptake induced neurotoxicity and oxidative stress in the common toad R. areanum. Thus, some blood biomarkers employed in our study (i.e. BChE, CbE, 2-PAM, and CAT) might be used as predictors in health and ecological risk assessment of amphibian populations exposed to CPF. |
topic |
rhinella arenarum blood chlorpyrifos dermal exposure b-esterases catalase |
url |
https://doi.org/10.2478/intox-2018-0011 |
work_keys_str_mv |
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1717781357062520832 |