A Structurally Variable Hinged Tetrahedron Framework from DNA Origami
Nanometer-sized polyhedral wire-frame objects hold a wide range of potential applications both as structural scaffolds as well as a basis for synthetic nanocontainers. The utilization of DNA as basic building blocks for such structures allows the exploitation of bottom-up self-assembly in order to a...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2011-01-01
|
Series: | Journal of Nucleic Acids |
Online Access: | http://dx.doi.org/10.4061/2011/360954 |
id |
doaj-f98af5ed9780451ebdd6248707898a61 |
---|---|
record_format |
Article |
spelling |
doaj-f98af5ed9780451ebdd6248707898a612020-11-24T22:00:44ZengHindawi LimitedJournal of Nucleic Acids2090-021X2011-01-01201110.4061/2011/360954360954A Structurally Variable Hinged Tetrahedron Framework from DNA OrigamiDavid M. Smith0Verena Schüller1Carsten Forthmann2Robert Schreiber3Philip Tinnefeld4Tim Liedl5Physik Weicher Materie und Biophysik, Ludwig Maximilian University, 80539 Munich, GermanyPhysik Weicher Materie und Biophysik, Ludwig Maximilian University, 80539 Munich, GermanyAngewandte Physik-Biophysik, Ludwig Maximilian University, 80539 Munich, GermanyPhysik Weicher Materie und Biophysik, Ludwig Maximilian University, 80539 Munich, GermanyCenter for NanoScience, Ludwig Maximilian University, 80799 Munich, GermanyPhysik Weicher Materie und Biophysik, Ludwig Maximilian University, 80539 Munich, GermanyNanometer-sized polyhedral wire-frame objects hold a wide range of potential applications both as structural scaffolds as well as a basis for synthetic nanocontainers. The utilization of DNA as basic building blocks for such structures allows the exploitation of bottom-up self-assembly in order to achieve molecular programmability through the pairing of complementary bases. In this work, we report on a hollow but rigid tetrahedron framework of 75 nm strut length constructed with the DNA origami method. Flexible hinges at each of their four joints provide a means for structural variability of the object. Through the opening of gaps along the struts, four variants can be created as confirmed by both gel electrophoresis and direct imaging techniques. The intrinsic site addressability provided by this technique allows the unique targeted attachment of dye and/or linker molecules at any point on the structure's surface, which we prove through the superresolution fluorescence microscopy technique DNA PAINT.http://dx.doi.org/10.4061/2011/360954 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
David M. Smith Verena Schüller Carsten Forthmann Robert Schreiber Philip Tinnefeld Tim Liedl |
spellingShingle |
David M. Smith Verena Schüller Carsten Forthmann Robert Schreiber Philip Tinnefeld Tim Liedl A Structurally Variable Hinged Tetrahedron Framework from DNA Origami Journal of Nucleic Acids |
author_facet |
David M. Smith Verena Schüller Carsten Forthmann Robert Schreiber Philip Tinnefeld Tim Liedl |
author_sort |
David M. Smith |
title |
A Structurally Variable Hinged Tetrahedron Framework from DNA Origami |
title_short |
A Structurally Variable Hinged Tetrahedron Framework from DNA Origami |
title_full |
A Structurally Variable Hinged Tetrahedron Framework from DNA Origami |
title_fullStr |
A Structurally Variable Hinged Tetrahedron Framework from DNA Origami |
title_full_unstemmed |
A Structurally Variable Hinged Tetrahedron Framework from DNA Origami |
title_sort |
structurally variable hinged tetrahedron framework from dna origami |
publisher |
Hindawi Limited |
series |
Journal of Nucleic Acids |
issn |
2090-021X |
publishDate |
2011-01-01 |
description |
Nanometer-sized polyhedral wire-frame objects hold a wide range of potential applications both as structural scaffolds as well as a basis for synthetic nanocontainers. The utilization of DNA as basic building blocks for such structures allows the exploitation of bottom-up self-assembly in order to achieve molecular programmability through the pairing of complementary bases. In this work, we report on a hollow but rigid tetrahedron framework of 75 nm strut length constructed with the DNA origami method. Flexible hinges at each of their four joints provide a means for structural variability of the object. Through the opening of gaps along the struts, four variants can be created as confirmed by both gel electrophoresis and direct imaging techniques. The intrinsic site addressability provided by this technique allows the unique targeted attachment of dye and/or linker molecules at any point on the structure's surface, which we prove through the superresolution fluorescence microscopy technique DNA PAINT. |
url |
http://dx.doi.org/10.4061/2011/360954 |
work_keys_str_mv |
AT davidmsmith astructurallyvariablehingedtetrahedronframeworkfromdnaorigami AT verenaschuller astructurallyvariablehingedtetrahedronframeworkfromdnaorigami AT carstenforthmann astructurallyvariablehingedtetrahedronframeworkfromdnaorigami AT robertschreiber astructurallyvariablehingedtetrahedronframeworkfromdnaorigami AT philiptinnefeld astructurallyvariablehingedtetrahedronframeworkfromdnaorigami AT timliedl astructurallyvariablehingedtetrahedronframeworkfromdnaorigami AT davidmsmith structurallyvariablehingedtetrahedronframeworkfromdnaorigami AT verenaschuller structurallyvariablehingedtetrahedronframeworkfromdnaorigami AT carstenforthmann structurallyvariablehingedtetrahedronframeworkfromdnaorigami AT robertschreiber structurallyvariablehingedtetrahedronframeworkfromdnaorigami AT philiptinnefeld structurallyvariablehingedtetrahedronframeworkfromdnaorigami AT timliedl structurallyvariablehingedtetrahedronframeworkfromdnaorigami |
_version_ |
1725842930936053760 |