Methylation Profile Difference in Human High-metastatic Large Cell Lung Cancer Cell Line L9981 and Low-metastatic Large Cell Lung Cancer Line NL9980
Background and objective Invasion and metastasis is one of malignant phenotype of lung cancer and the important cause of the death of lung cancer patients. The aim of this study is to explore the methylation profile difference in different metastatic potential cell lines. Methods To compare the DNA...
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Chinese Anti-Cancer Association; Chinese Antituberculosis Association
2010-08-01
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Online Access: | http://www.lungca.org/index.php?journal=01&page=article&op=viewFile&path[]=10.3779%2Fj.issn.1009-3419.2010.08.02&path[]=1640 |
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doaj-f99b455dfb2144b5be613d505d6abde32020-11-25T00:50:34ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34191999-61872010-08-01138763768Methylation Profile Difference in Human High-metastatic Large Cell Lung Cancer Cell Line L9981 and Low-metastatic Large Cell Lung Cancer Line NL9980Hui LVHuiqin YANMin WANGYongwen LIHaisu WANHongyu LIUHeng WUQinghua ZHOUBackground and objective Invasion and metastasis is one of malignant phenotype of lung cancer and the important cause of the death of lung cancer patients. The aim of this study is to explore the methylation profile difference in different metastatic potential cell lines. Methods To compare the DNA methylation profile of two large cell lung cancer cell lines with different metastatic potential by MeIP chip hybridization, hypermethylated and hypomethylated genes of L9981 cell lines were analyzed online in NIH-DAVID, KEGG and MILANO webside. Results Compared with NL9980 cell line, 735 genes are hypermethylated in L9981, including 656 known genes and 79 unknown genes; 809 gene are hypermethylated in L9981, including 698 known genes and 111 unknown genes; the different genes are involved in cell process, signal transduction, cell communication, cell adhesion, cell motility, and angiogenesis. Conclusion Hypermethylation of suppressor genes and negative regulator of signal transduction and hopomethylation of oncogene and cell adhesion molecules (CAMs) in L9981 may promote metastasis of tumor cells.http://www.lungca.org/index.php?journal=01&page=article&op=viewFile&path[]=10.3779%2Fj.issn.1009-3419.2010.08.02&path[]=1640MeIP chipDNA methylationMetastasisLung neoplasms |
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language |
zho |
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DOAJ |
author |
Hui LV Huiqin YAN Min WANG Yongwen LI Haisu WAN Hongyu LIU Heng WU Qinghua ZHOU |
spellingShingle |
Hui LV Huiqin YAN Min WANG Yongwen LI Haisu WAN Hongyu LIU Heng WU Qinghua ZHOU Methylation Profile Difference in Human High-metastatic Large Cell Lung Cancer Cell Line L9981 and Low-metastatic Large Cell Lung Cancer Line NL9980 Chinese Journal of Lung Cancer MeIP chip DNA methylation Metastasis Lung neoplasms |
author_facet |
Hui LV Huiqin YAN Min WANG Yongwen LI Haisu WAN Hongyu LIU Heng WU Qinghua ZHOU |
author_sort |
Hui LV |
title |
Methylation Profile Difference in Human High-metastatic Large Cell Lung Cancer Cell Line L9981 and Low-metastatic Large Cell Lung Cancer Line NL9980 |
title_short |
Methylation Profile Difference in Human High-metastatic Large Cell Lung Cancer Cell Line L9981 and Low-metastatic Large Cell Lung Cancer Line NL9980 |
title_full |
Methylation Profile Difference in Human High-metastatic Large Cell Lung Cancer Cell Line L9981 and Low-metastatic Large Cell Lung Cancer Line NL9980 |
title_fullStr |
Methylation Profile Difference in Human High-metastatic Large Cell Lung Cancer Cell Line L9981 and Low-metastatic Large Cell Lung Cancer Line NL9980 |
title_full_unstemmed |
Methylation Profile Difference in Human High-metastatic Large Cell Lung Cancer Cell Line L9981 and Low-metastatic Large Cell Lung Cancer Line NL9980 |
title_sort |
methylation profile difference in human high-metastatic large cell lung cancer cell line l9981 and low-metastatic large cell lung cancer line nl9980 |
publisher |
Chinese Anti-Cancer Association; Chinese Antituberculosis Association |
series |
Chinese Journal of Lung Cancer |
issn |
1009-3419 1999-6187 |
publishDate |
2010-08-01 |
description |
Background and objective Invasion and metastasis is one of malignant phenotype of lung cancer and the important cause of the death of lung cancer patients. The aim of this study is to explore the methylation profile difference in different metastatic potential cell lines. Methods To compare the DNA methylation profile of two large cell lung cancer cell lines with different metastatic potential by MeIP chip hybridization, hypermethylated and hypomethylated genes of L9981 cell lines were analyzed online in NIH-DAVID, KEGG and MILANO webside. Results Compared with NL9980 cell line, 735 genes are hypermethylated in L9981, including 656 known genes and 79 unknown genes; 809 gene are hypermethylated in L9981, including 698 known genes and 111 unknown genes; the different genes are involved in cell process, signal transduction, cell communication, cell adhesion, cell motility, and angiogenesis. Conclusion Hypermethylation of suppressor genes and negative regulator of signal transduction and hopomethylation of oncogene and cell adhesion molecules (CAMs) in L9981 may promote metastasis of tumor cells. |
topic |
MeIP chip DNA methylation Metastasis Lung neoplasms |
url |
http://www.lungca.org/index.php?journal=01&page=article&op=viewFile&path[]=10.3779%2Fj.issn.1009-3419.2010.08.02&path[]=1640 |
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